| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,101,909 | T→C | 21.0% | Y335Y (TAT→TAC) ‡ | uvrD → | DNA‑dependent ATPase I/helicase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,101,909 | 0 | T | C | 21.0% | 32.4 / 4.5 | 19 | Y335Y (TAT→TAC) ‡ | uvrD | DNA‑dependent ATPase I/helicase II |
| Reads supporting (aligned to +/- strand): ref base T (8/7); new base C (2/2); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01 | |||||||||||
TCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATCCCCTACCGCATCTATGGC > NC_002947/6101766‑6102047 | tCCACCGGCGGCATACTCAAGGCGGCCAACGCGCGGAGCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTg < 2:248876/150‑1 (MQ=255) tCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGcc > 1:72676/1‑150 (MQ=255) aaGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTg < 2:83099/150‑1 (MQ=255) gcTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCaa > 1:304307/1‑150 (MQ=255) gcTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCaa > 2:423794/1‑150 (MQ=255) ggCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCa < 2:110815/150‑1 (MQ=255) gggCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAATCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTa < 1:142862/150‑1 (MQ=255) gggCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACATAGCGCGCTTCGTCGTgct > 1:6039/1‑91 (MQ=38) gggCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACATAGCGCGCTTCGTCGTgct < 2:6039/93‑3 (MQ=38) gAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACATAGCGCGCTTCGTCGTgctc < 1:413450/87‑4 (MQ=37) gAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACATAGCGCGCTTCGTCGTgctc > 2:413450/1‑84 (MQ=37) cTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGTGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAAc < 2:40315/150‑1 (MQ=255) aCCGACATGGGCGAAGGCGAACTACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAg > 1:413512/1‑150 (MQ=255) aCATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTcgc < 2:304307/150‑1 (MQ=255) gaaggcgaaCCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTg > 1:387142/1‑63 (MQ=255) gaaggcgaaCCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTg < 2:387142/63‑1 (MQ=255) cACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCGTCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGccc > 2:214846/1‑150 (MQ=255) gacgaAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTAGCCGACGAAGGGCGAGGCGGTGTTGCGGTAAGAGGCCCTGCTGCGCGAGCGCATCCCCTACCGCATCTAt > 1:386674/1‑150 (MQ=255) gaAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATCCCCTACCGCATCTATggc > 2:104866/1‑150 (MQ=255) | TCCACCGGCGGCATCCTCAAGGCGGCCAACGCGCTGATCGCCAACAACAGCGGGCGCCTGGGCAAGGAACTGTGGACCGACATGGGCGAAGGCGAACCACTGACCCTGTACGCGGCCTACAACGAGCACGACGAAGCGCGCTATGTCGTGGAAACCATCGAGAGCCTGGTCAAGCAGGGCAATGCGCGCAACGAGATCGCCATCCTGTACCGTTCCAACGCCCAGTCGCGCGTGCTGGAAGAGGCCCTGCTGCGCGAGCGCATCCCCTACCGCATCTATGGC > NC_002947/6101766‑6102047 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |