Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,129,211 A→C 12.9% intergenic (+17/+50) PP_5376 → / ← PP_5377 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,129,2110AC12.9% 74.9 / 2.3 29intergenic (+17/+50)PP_5376/PP_5377hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (8/17);  new base C (2/2);  total (10/19)
Fisher's exact test for biased strand distribution p-value = 5.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACC  >  NC_002947/6129063‑6129356
                                                                                                                                                    |                                                                                                                                                 
gTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCaa                                                                                                                                                  >  2:210826/1‑150 (MQ=255)
        tgtgACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAAc                                                                                                                                          >  1:366020/1‑150 (MQ=255)
           gACCTCGACCCGGGGAAGCTGTCACTAAATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAACCGgc                                                                                                                                       <  2:52869/150‑1 (MQ=255)
              cTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCAGACCATATTGATCTCTGAATGCTGTCCAAACGAAAACGGCGcc                                                                                                                                    >  1:339139/1‑150 (MQ=255)
                      ggggAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAgc                                                                                                                            >  2:294421/1‑150 (MQ=255)
                                tgACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCg                                                                                                                  <  1:210826/150‑1 (MQ=255)
                                  aCGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGtt                                                                                                                <  2:366020/150‑1 (MQ=255)
                                  aCGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGtt                                                                                                                >  2:177740/1‑150 (MQ=255)
                                    gTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTgg                                                                                                              <  2:424007/150‑1 (MQ=255)
                                                         tATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGcc                                                                                         >  1:89034/1‑150 (MQ=255)
                                                          aTGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCt                                                                                        <  2:280492/150‑1 (MQ=255)
                                                                  tCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATActc                                                                                <  1:88830/150‑1 (MQ=255)
                                                                   cGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATActct                                                                               <  1:22991/150‑1 (MQ=255)
                                                                   cGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATActct                                                                               <  2:344089/150‑1 (MQ=255)
                                                                    gACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCCAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGtttg                                                                                                              >  1:114206/1‑116 (MQ=37)
                                                                    gACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCCAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGtttg                                                                                                              <  2:114206/118‑3 (MQ=37)
                                                                           aaGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCAc                                                                       >  1:346594/1‑150 (MQ=255)
                                                                                 aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATc                                                                 >  1:337420/1‑150 (MQ=255)
                                                                                 aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATc                                                                 <  1:294421/150‑1 (MQ=255)
                                                                                 aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATc                                                                 <  1:100907/150‑1 (MQ=255)
                                                                                        tcGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGCTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCg                                                          >  2:5628/1‑150 (MQ=255)
                                                                                                              cAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCt                                    <  1:189803/150‑1 (MQ=255)
                                                                                                                 ttGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCCAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGTTTGGa                                                                                                            >  2:277904/1‑75 (MQ=2)
                                                                                                                 ttGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCCAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGTTTGGa                                                                                                            <  1:277904/75‑1 (MQ=11)
                                                                                                                           cAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgaga                       <  2:288649/150‑1 (MQ=255)
                                                                                                                              aaTTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGac                    <  2:337420/150‑1 (MQ=255)
                                                                                                                                   aGCTCTGAAGGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCAt               <  2:392113/150‑1 (MQ=255)
                                                                                                                                      tctGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCa            <  2:346594/150‑1 (MQ=255)
                                                                                                                                       ctGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCAc           <  2:89034/150‑1 (MQ=255)
                                                                                                                                                gTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATAcc  <  1:5628/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GTGATTGCTGTGACCTCGACCCGGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACC  >  NC_002947/6129063‑6129356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑