Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,129,242 G→T 17.9% intergenic (+48/+19) PP_5376 → / ← PP_5377 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,129,2420GT17.9% 68.7 / 5.7 28intergenic (+48/+19)PP_5376/PP_5377hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (7/16);  new base T (2/3);  total (9/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01

TGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTGGGACGGCG  >  NC_002947/6129095‑6129387
                                                                                                                                                   |                                                                                                                                                 
tgACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCg                                                                                                                                                 <  1:210826/150‑1 (MQ=255)
  aCGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGtt                                                                                                                                               <  2:366020/150‑1 (MQ=255)
  aCGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGtt                                                                                                                                               >  2:177740/1‑150 (MQ=255)
    gTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTgg                                                                                                                                             <  2:424007/150‑1 (MQ=255)
                         tATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGcc                                                                                                                        >  1:89034/1‑150 (MQ=255)
                          aTGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCt                                                                                                                       <  2:280492/150‑1 (MQ=255)
                                  tCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATActc                                                                                                               <  1:88830/150‑1 (MQ=255)
                                   cGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATActct                                                                                                              <  2:344089/150‑1 (MQ=255)
                                   cGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATActct                                                                                                              <  1:22991/150‑1 (MQ=255)
                                    gACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCCAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGtttg                                                                                                                                             >  1:114206/1‑116 (MQ=37)
                                    gACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCCAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGtttg                                                                                                                                             <  2:114206/118‑3 (MQ=37)
                                           aaGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCAc                                                                                                      >  1:346594/1‑150 (MQ=255)
                                                 aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATc                                                                                                >  1:337420/1‑150 (MQ=255)
                                                 aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATc                                                                                                <  1:294421/150‑1 (MQ=255)
                                                 aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATc                                                                                                <  1:100907/150‑1 (MQ=255)
                                                        tcGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGCTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCg                                                                                         >  2:5628/1‑150 (MQ=255)
                                                                              cAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCt                                                                   <  1:189803/150‑1 (MQ=255)
                                                                                 ttGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCCAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGTTTGGa                                                                                                                                           <  1:277904/75‑1 (MQ=11)
                                                                                 ttGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCCAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGTTTGGa                                                                                                                                           >  2:277904/1‑75 (MQ=2)
                                                                                           cAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgaga                                                      <  2:288649/150‑1 (MQ=255)
                                                                                              aaTTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGac                                                   <  2:337420/150‑1 (MQ=255)
                                                                                                   aGCTCTGAAGGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCAt                                              <  2:392113/150‑1 (MQ=255)
                                                                                                      tctGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCa                                           <  2:346594/150‑1 (MQ=255)
                                                                                                       ctGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCAc                                          <  2:89034/150‑1 (MQ=255)
                                                                                                                gTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATAcc                                 <  1:5628/150‑1 (MQ=255)
                                                                                                                          aaaCGGCGCTTTTAAGGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCt                       >  1:331584/1‑150 (MQ=255)
                                                                                                                                  ccTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCAt               >  1:151385/1‑150 (MQ=255)
                                                                                                                                               gTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTGGGACGGCg  <  1:177740/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTGGGACGGCG  >  NC_002947/6129095‑6129387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑