| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 358,726 | T→C | 21.0% | W64R (TGG→CGG) ‡ | gbdR → | AraC family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 358,726 | 0 | T | C | 21.0% | 28.7 / 4.9 | 20 | W64R (TGG→CGG) ‡ | gbdR | AraC family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (9/6); new base C (2/2); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.38e-01 | |||||||||||
AACACCCCAGTCCATCGGGTTTCTTCTATTGGACAATTTCACCCTGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAATCACACCGGATGCCGCAATCAGCAATGCTCCGCCCATCGACACCGTGATTGTCTGTGGTGGTGTTGGCATCCAGCGTTCCGTCACCCGTGAACACGTCACCTGGTTGCAAGCCCA > NC_002947/358578‑358862 | aacaCCCCAGTCCATCGGGTTTCTTCTATTGGACAATTTCACCCTGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGtgtg < 2:140167/150‑1 (MQ=255) cAGTCCATCGGGTTTCTTCTATTGGACAATTTCACCCTGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGc < 1:367007/150‑1 (MQ=255) aTCGGGTTTCTTCTATTGGACAATTTCACCCTGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGCGATgac < 1:70407/150‑3 (MQ=255) tcttctATTGGACAATTTCACCCTGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAAt > 2:217750/1‑150 (MQ=255) ggACAATTTCACCCTGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCAAGAGCGTGGTGTCCGGCGATGGCCTGCAAATCACACCGGa > 2:35801/1‑150 (MQ=255) gACAATTTCACCCTGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGCAGGCCATCGCTGGcccaca < 2:37341/137‑5 (MQ=255) gACAATTTCACCCTGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGCAGGCCATCGCTGGcccaca > 1:37341/1‑133 (MQ=255) ccTGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAATCACACCGGATGCCGCAATCAg > 1:330616/1‑150 (MQ=255) cTGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGCAGGCCATCGCTGGcccacac < 2:303564/126‑6 (MQ=255) cTGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCAGTCGGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGCAGGCCATCGCTGGcccacac > 1:303564/1‑121 (MQ=255) tGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTCAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGCGCTGGCCTGCACATCACACCGGATGCCGCAATCAGCa > 2:77899/1‑150 (MQ=255) gAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAATCACACCAGATACTGCAATCAGCAATGCTCCGCCCATCGACACCGTg < 1:35801/150‑1 (MQ=255) cTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAATCACACCGGATGCCGCAATCAGCAATGCTCCGCCCATCGACACCGTGATTGTc > 2:443402/1‑150 (MQ=255) tGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAATCACACCGGATGCCGCAATCAGCAATGCTCCGCCCATCGACACCGTGATTGTCt > 2:357990/1‑150 (MQ=255) gCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAATCACACCGGATGCCGCAATCAGCAATGCTCCGCCCATCGACACCGTGATTGTCTGTGGTGGTGTTGGCATCCAGCGtt < 1:443402/150‑1 (MQ=255) tGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAATCACACCGGATGCCGCAATCAGCAATGCTCCGCCCATCGACACCGTGATTGTCTGTGGTGGTGTTGGCATCCAGCGTTCCGTCAccc > 2:372275/1‑150 (MQ=255) acacGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAATCACACCGGATGCCGCAATCAGCAATGCTCCGCCCATCGACACCGTGATTGTCTGTGGTGGTGTTGGCATCCAGCGTTCCGTCACCCGTGAACACGTCa > 1:263653/1‑150 (MQ=255) tGACGATCGATGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAATCACACCGGATGCCGCAATCAGCAATGCTCCGCCCATCGACACCGTGATTGTCTGTGGTGGTGTTGGCATCCAGCGTTCCGTCACCCGTGAACACGTCACCTGGt > 1:38233/1‑150 (MQ=255) gatcgatGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAATCACACCGGATGCCGCAATCAGCAATGCTCCGCCCATCGACACCGTGATTGTCTGTGGTGGTGTTGGCATCCAGCGTTCCGTCACCCGTGAACACGTCACCTGGTTGCa > 1:18542/1‑150 (MQ=255) atcgatGGCGGCCAGGTGTTGGCCAGCGATGGCCTGCAAATCACACCGGATGCCGCAATCAGCAATGCTCCGCCCATCGACACCGTGATTGTCTGTGGTGGTGTTGGCATCCAGCGTTCCGTCACCCGTGAACACGTCACCTGGTTGCaa < 2:263653/150‑1 (MQ=255) tGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAATCACACCGGATGCCGCAATCAGCAATGCTCCGCCCATCGACACCGTGATTGTCTGTGGTGGTGTTGGCATCCAGCGTTCCGTCACCCGTGAACACGTCACCTGGTTGCAAGCCCa < 2:368958/150‑1 (MQ=255) | AACACCCCAGTCCATCGGGTTTCTTCTATTGGACAATTTCACCCTGATTTCCCTGGCATCGGCGGTCGAGCCGCTGCGCATGGCCAACCAGCTGTCTGGCCGTGAGCTGTATCGCTGGCACACGCTGACGATCGATGGCGGCCAGGTGTGGGCCAGCGATGGCCTGCAAATCACACCGGATGCCGCAATCAGCAATGCTCCGCCCATCGACACCGTGATTGTCTGTGGTGGTGTTGGCATCCAGCGTTCCGTCACCCGTGAACACGTCACCTGGTTGCAAGCCCA > NC_002947/358578‑358862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |