| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 141,137 | T→A | 19.0% | Q237L (CAG→CTG) | algB ← | alginate biosynthesis transcriptional regulator AlgB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 141,137 | 0 | T | A | 19.0% | 44.6 / 6.3 | 21 | Q237L (CAG→CTG) | algB | alginate biosynthesis transcriptional regulator AlgB |
| Reads supporting (aligned to +/- strand): ref base T (7/10); new base A (2/2); total (9/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
CAGGATACGTACATCGGCACGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCG‑GCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTGCGTGCACGCTTGCTCCAACCATG > NC_002947/140995‑141282 | cAGGATACGTACATCGGCACGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGCTTTCACCCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGAc < 1:140608/150‑1 (MQ=255) ggATACGTACATCGGCACGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCAGGCTGAcgg < 1:395287/150‑3 (MQ=255) ggTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGgc > 1:119566/1‑150 (MQ=255) aCCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCg < 1:217440/150‑1 (MQ=255) tCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGCTGAAg < 1:131812/150‑1 (MQ=255) cgTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCgg < 1:393917/150‑1 (MQ=255) ttCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGt > 1:320875/1‑150 (MQ=255) ttCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGt < 2:280395/150‑1 (MQ=255) tCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTa < 2:119566/150‑1 (MQ=255) gCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCG‑GCGTTAAGCGACGGGCAGt > 1:190602/1‑150 (MQ=255) gAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCG‑GCGTTAAGCGACGGGCAGTTGAt < 1:209583/150‑1 (MQ=255) cgtcgAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGTTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCG‑GCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCtt > 2:357471/1‑150 (MQ=255) gatctcGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCG‑GCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTGCGTGCa < 1:427151/145‑1 (MQ=255) gTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCG‑GCTTTAAGCGACGGGCAGTTGATGGTCACGCATGCTTTTCGTGCAcagatc > 2:427151/1‑145 (MQ=255) cccGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCgg < 1:349983/61‑1 (MQ=255) cccGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCgg > 2:349983/1‑61 (MQ=255) gggTCAGCCAGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCttt < 1:175972/80‑1 (MQ=255) gggTCAGCCAGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCttt > 2:175972/3‑82 (MQ=255) gggTCAGCCAGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCG‑GCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTGCGTGCACGCTTGCTCCaa > 2:350125/3‑150 (MQ=255) ccGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGGGTTCGTTCCATCAGATCGAACGTTAGGAGA‑TAGCTGGTGATGGTCACGCAGGCTTTGCGTGCACGCTTGCTCCaa > 1:433587/1‑150 (MQ=255) aGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCG‑GCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTGCGTGCACGCTTGCTCCAACCatg < 2:190602/150‑1 (MQ=255) | CAGGATACGTACATCGGCACGCCGGGTAACCGGGTCGCCGACGCGTTCGTATTCCTTGTCCTGGATGAAACGCAACAGTTTGGGCTGCAAGGTCAGCGGGAAATCGCCGATCTCGTCGAGGAATAGCGTGCCCCCGTCAGCCTGGCTGACCCGCCCGAGGGTGCTTTCGCTGGCGCCGGTGAAGGCGCCGCGGGTATGACCGAATAGCTCGCTTTCCATCAGCTCG‑GCGTTAAGCGACGGGCAGTTGATGGTCACGCAGGCTTTGCGTGCACGCTTGCTCCAACCATG > NC_002947/140995‑141282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |