Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 441,931 C→T 9.1% P270L (CCG→CTG)  bioF → 8‑amino‑7‑oxononanoate synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947441,9310CT 9.1% 117.0 / 3.2 44P270L (CCG→CTG) bioF8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base C (21/19);  new base T (2/2);  total (23/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

GCCTGGGCGTGGATGATGTACCCGTGCTGATCGGCACGCTGGGCAAGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATGGACAGCCCGACGCCGATC  >  NC_002947/441784‑442076
                                                                                                                                                   |                                                                                                                                                 
gCCTGGGCGTGGATGATGTACCCGTGCTGATCGGCACGCTGGGCAAGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGGACCGACTCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTCCACCACCAGCCAGCCACCgg                                                                                                                                                 >  1:140764/1‑150 (MQ=255)
         tGGATGATGTACCCGTGCTGATCGGCACGCTGGGCAAGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACATTTCGCCCGCCCCGATATCTACACCACCAGCCAGCCACCGGCGCTGGCCt                                                                                                                                        <  2:197425/150‑1 (MQ=255)
             tgatgTACCCGTGCTGATCGGCACGCTGGGCAAGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTgcgc                                                                                                                                    <  2:337913/150‑1 (MQ=255)
                   aCCCGTGCTGATCGGCACGCTGGGCAAGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACt                                                                                                                              >  1:97518/1‑150 (MQ=255)
                        tGCTGATCGGCACGCTGGGCAAGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCAAGAAACCGGCGCTGGCCTGCGCCACACTCAaga                                                                                                                         >  1:335246/1‑150 (MQ=255)
                           tGATCGGCACGCTGGGCAAGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGcc                                                                                                                      >  2:175603/1‑150 (MQ=255)
                                ggCACGCTGGGCAAGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAaga                                                                                                                         <  1:193291/142‑1 (MQ=255)
                                ggCACGCTGGGCAAGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAaga                                                                                                                         >  2:193291/1‑142 (MQ=255)
                                    cGCTGGGCAAGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGt                                                                                                             <  2:140764/150‑1 (MQ=255)
                                     gCTGGGCAAGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGtt                                                                                                            >  1:115174/1‑150 (MQ=255)
                                            aaGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGcgccgc                                                                                                     >  1:253911/1‑150 (MQ=255)
                                                    cGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTg                                                                                             <  1:297375/150‑1 (MQ=255)
                                                                   cTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCt                                                                              >  1:273654/1‑150 (MQ=255)
                                                                    tttGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTg                                                                             <  2:97518/150‑1 (MQ=255)
                                                                      tgttgCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGc                                                                           <  2:253911/150‑1 (MQ=255)
                                                                        ttgCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTg                                                                                                                                            >  2:219264/1‑83 (MQ=255)
                                                                        ttgCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTg                                                                                                                                            <  1:219264/83‑1 (MQ=255)
                                                                            cGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCtt                                                                     >  2:365403/1‑150 (MQ=255)
                                                                              gCAGGGAGACGCTAATAGAGGCGCTGGTACAGGTAGCCAGCCCGTATATCTACACAACCAGCCATCCACCGGCGCTAGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCAGCGCAACCTGGCGTCGCGAGCACCTGGCAGCCTTGa                                                                   >  2:131364/1‑150 (MQ=255)
                                                                              gCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGa                                                                   <  1:53403/150‑1 (MQ=255)
                                                                               cAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGAt                                                                  <  1:147444/150‑1 (MQ=255)
                                                                                          tGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGAAGCAGTTGCGCAGCACAACCTGCGCTCGGGTGCTCCGGCGTGCCTTGATTCGCCAGTTca                                                       >  2:407133/1‑149 (MQ=255)
                                                                                            aTCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACCCAACCGGCCCGCCAACGGCGCTGATCTGCACCCCTTTTCGGATTATGTTGCGGTTTTGCCGCGTAACCTGGCGTCGGGAGCACTTGGCAGCCTTGATTCGCCAGTTCCga                                                     <  2:273654/150‑1 (MQ=255)
                                                                                                          aCAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCgcagca                                       >  2:56844/1‑150 (MQ=255)
                                                                                                                             ctCTACACCACCAGCCAGCCACTGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATg                    <  2:121510/149‑1 (MQ=255)
                                                                                                                             ctCTACACCACCAGCCAGCCACTGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATg                    <  2:121556/149‑1 (MQ=255)
                                                                                                                                tACACCACCAGCCAGCCACTGGCGCTGGCGTGCGACCCATTCGAGGACGTAGAGCGGATGCGCCGCGGAATCGGGTTTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATGGag                 >  1:121510/1‑149 (MQ=255)
                                                                                                                                tACACCACCAGCCAGCCACTGGCGCTGGCCTGCGCCACACTCCAGCGAAAGCAGCTGCGGCGCCGCCAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATGGag                 >  1:121556/1‑149 (MQ=255)
                                                                                                                                tACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATGGAc                 >  2:335619/1‑150 (MQ=255)
                                                                                                                                 acacCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTggg                                 >  1:405563/1‑133 (MQ=255)
                                                                                                                                 acacCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTggg                                 <  2:405563/133‑1 (MQ=255)
                                                                                                                                    ccaccaGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATGGACAGcc             >  1:385769/1‑150 (MQ=255)
                                                                                                                                     caccaGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGaaa                                                                                                 <  2:254287/65‑1 (MQ=255)
                                                                                                                                     caccaGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGaaa                                                                                                 >  1:254287/1‑65 (MQ=255)
                                                                                                                                     caccaGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATGGACAGccc            >  1:333191/1‑150 (MQ=255)
                                                                                                                                     caccaGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAACATCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATGGACAGccc            <  1:407133/150‑1 (MQ=255)
                                                                                                                                      accaGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATGGAc                 >  2:280114/1‑144 (MQ=255)
                                                                                                                                      accaGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATGGAc                 <  1:280114/144‑1 (MQ=255)
                                                                                                                                         agccagccACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGcgtcgt                                                                                      >  2:426164/1‑72 (MQ=255)
                                                                                                                                         agccagccACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGcgtcgt                                                                                      <  1:426164/72‑1 (MQ=255)
                                                                                                                                          gccagccaCCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTGGATGAACAGCCCGACGc       <  2:52271/150‑1 (MQ=255)
                                                                                                                                           ccagccaCCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGcgccgc                                                                                                     <  2:47949/55‑1 (MQ=255)
                                                                                                                                           ccagccaCCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGcgccgc                                                                                                     >  1:47949/1‑55 (MQ=255)
                                                                                                                                               ccaCCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATGGACAGCCCGACGCCGATc  <  1:175603/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GCCTGGGCGTGGATGATGTACCCGTGCTGATCGGCACGCTGGGCAAGGCTTGCGGCACTGCGGGCGCCTTTGTTGCCGGCAGCGAGGCGCTGATCGAGGCGCTGGTACAGTTCGCCCGCCCCTATATCTACACCACCAGCCAGCCACCGGCGCTGGCCTGCGCCACACTCAAGAGCCTGGAGCTGTTGCGCCGCGAAACCTGGCGTCGTGAGCACCTGGCAGCCTTGATTCGCCAGTTCCGAGAAGGCGCGCAGCAGTTGGGGCTGCAGTTGATGGACAGCCCGACGCCGATC  >  NC_002947/441784‑442076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑