| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 540,121 | C→T | 14.3% | R832R (CGC→CGT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 540,121 | 0 | C | T | 14.3% | 62.4 / 4.0 | 28 | R832R (CGC→CGT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base C (13/11); new base T (2/2); total (15/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01 | |||||||||||
ACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAGGAAATCACTGCGGACATCCCGAACGTGGGTGAAGCCGCACTGAACAAGCTGGACGAAGCCGGTATCGTTTACGTAG > NC_002947/539994‑540260 | aCGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATcc < 2:356650/141‑1 (MQ=255)aCGGTCCGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATcc > 1:356650/1‑141 (MQ=255)aCGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAgg < 1:28468/150‑1 (MQ=255) tccGTCCACCGACCTCGGTGAACTGGCGCTTGGTCAGAACATGCGCGTAGCGTTCATGCCGTGGAATGGTTACAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACt > 1:94495/2‑150 (MQ=255) cccGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACt > 1:59276/1‑150 (MQ=255) gACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCtgtgtg < 1:264069/150‑1 (MQ=255) gggTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGcgcg < 1:337235/150‑1 (MQ=255) gTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGcc > 2:284/1‑150 (MQ=255) tCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGccc > 1:280410/1‑150 (MQ=255) cAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCa < 2:280410/150‑1 (MQ=255) gTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACTCCAAGCTCGGccc < 2:262834/132‑1 (MQ=255) gTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGccc > 1:262834/1‑132 (MQ=255) tGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAgg > 2:95183/1‑132 (MQ=255) tGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAgg < 1:95183/132‑1 (MQ=255) gTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAGGaa < 1:255341/130‑1 (MQ=255) gTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAGGaa > 2:255341/1‑130 (MQ=255) acAACTTCGAAGACTCCATCCTCGTATCCGAGCGTGTTGTTCAGGAAGACCGTTTCACCACCATCCACATTCAGGAACTGGCGTGTGTGTCCCGTGACACCAAGCTGGGTCCGGAAGAGATCACCGCTGACATCCCGAACGTGGGTGAAg < 2:94495/149‑1 (MQ=255) cAACTTCGAAGACTCCATCTGCCTGTCCGATCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAGGAAATCACTGCGGACATCCCGAACGTGGGTGAAGc < 1:284/150‑1 (MQ=255) cTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAGGAAATCACTGCGGACATCCCGAACGTGGGTGAAGCCGc > 2:237982/1‑150 (MQ=255) tCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCGCGGCACAGAGGAAATCACTGCGGACATCCCGAACGTGGGTGAAGCCGCACTGAACAAg > 1:140366/1‑150 (MQ=255) aTCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAGGAAATCACTGCg > 1:334668/1‑111 (MQ=255) aTCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAGGAAATCACTGCg < 2:334668/111‑1 (MQ=255) ccTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGAACTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAGGAAATCACTGCGGACATACCGAACGTGGGTGAAGCCGCACTGAACAAGCTGGACGa > 2:190577/1‑150 (MQ=255) ttCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCaga < 2:266606/76‑1 (MQ=255) ttCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCaga > 1:266606/1‑76 (MQ=255) aGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAGGAAATCACTGCGGACATCCCGAACGTGGGTGAAGCCGCACTGAACAAGCTGGACGAAGCCGGTAATGACTACGTAg > 2:95545/1‑150 (MQ=255) gCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAGGAAATCACTGCGGACATCCCGAACGTGGGTGAAGCCGCACTGAACAAGCTg < 1:426513/116‑1 (MQ=255) gCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAGGAAATCACTGCGGACATCCCGAACGTGGGTGAAGCCGCACTGAACAAGCTg > 2:426513/1‑116 (MQ=255) | ACGGCCCGTCCACCGACATGGGTGAGCTGGCTCTGGGTCAGAACATGCGCATCGCGTTCATGGCGTGGAACGGCTTCAACTTCGAAGACTCCATCTGCCTGTCCGAGCGTGTTGTTCAGGAAGATCGCTTCACCACCATCCACATTCAGGAACTGACCTGTGTGGCGCGTGACACCAAGCTCGGCCCAGAGGAAATCACTGCGGACATCCCGAACGTGGGTGAAGCCGCACTGAACAAGCTGGACGAAGCCGGTATCGTTTACGTAG > NC_002947/539994‑540260 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |