| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,851,762 | C→G | 25.1% | S269W (TCG→TGG) | relA → | ATP:GTP 3'‑pyrophosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,851,762 | 0 | C | G | 25.1% | 23.8 / 6.7 | 16 | S269W (TCG→TGG) | relA | ATP:GTP 3'‑pyrophosphotransferase |
| Reads supporting (aligned to +/- strand): ref base C (5/7); new base G (2/2); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
TCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGC > NC_002947/1851630‑1851893 | tCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGc > 1:301793/1‑150 (MQ=255) cTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCaaa < 1:242777/150‑1 (MQ=255) tGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAg > 1:441121/1‑150 (MQ=255) tGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa < 1:274077/150‑1 (MQ=255) aGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGt < 2:114415/150‑1 (MQ=255) tGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCt > 1:30351/1‑136 (MQ=255) tGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCt < 2:30351/136‑1 (MQ=255) aGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtccggtg > 1:427372/1‑150 (MQ=255) aaCGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGTGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgtgcgtgcggtgcgcgtgctggtgccggaaat > 1:81113‑M1/1‑113 (MQ=255) cGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgtgcgtgcggtgcgcgtgctggtgccggaaatcc < 1:60958‑M1/150‑40 (MQ=255) aCTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGTGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgtgcgtgcggtgcgcgtgctggtgccggaaatccgcgactgctacac < 2:81113‑M1/150‑53 (MQ=255) gggCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgtgcgtgcggtgcgcgtgctggtgccggaaatccgcgactgctacaccgcgctgggcatcgtgcacaccttgtgg < 2:441121‑M1/150‑81 (MQ=255) atatCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgtgcgtgcggtgcgcgtgctggtgccggaaatccgcgactgctacaccgcgctgg > 1:110986‑M1/1‑61 (MQ=255) atatCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgtgcgtgcggtgcgcgtgctggtgccggaaatccgcgactgctacaccgcgctgg < 2:110986‑M1/121‑61 (MQ=255) aTTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgtgcgtgcggtgcgcgtgctggtgccggaaatccgcgactgctacaccgcgctgg < 1:94717‑M1/115‑61 (MQ=255) aTTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgcgtgcgtgcggtgcgcgtgctggtgccggaaatccgcgactgctacaccgcgctgg > 2:94717‑M1/1‑55 (MQ=255) | TCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGC > NC_002947/1851630‑1851893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |