Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,851,762 C→G S269W (TCG→TGG)  relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,851,7620CG99.2% 813.5 / ‑0.8 240coding (806/2241 nt)relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (135/102);  minor base . (0/2);  total (136/104)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

AGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAG  >  NC_002947/1851618‑1851907
                                                                                                                                                |                                                                                                                                                 
aGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCt                                                                                                                                              <  1:3486375/150‑1 (MQ=255)
aGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCt                                                                                                                                              <  2:2025932/150‑1 (MQ=255)
 gTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTg                                                                                                                                             <  2:1486681/150‑1 (MQ=255)
  tACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTgg                                                                                                                                            >  2:1062488/1‑150 (MQ=255)
   aCAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGgc                                                                                                                                           <  2:6328821/150‑1 (MQ=255)
   aCAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGgc                                                                                                                                           <  1:6173498/150‑1 (MQ=255)
     aaGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGgcgc                                                                                                                                         >  2:551092/1‑150 (MQ=255)
     aaGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGgcgc                                                                                                                                         >  1:4115616/1‑150 (MQ=255)
      aGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCa                                                                                                                                        >  1:2022360/1‑150 (MQ=255)
        cAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAg                                                                                                                                      <  1:2368910/150‑1 (MQ=255)
          gATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGCAACATATCTATTGGATCTGGCGCAAGAt                                                                                                                                    >  1:917237/1‑150 (MQ=255)
          gATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGAt                                                                                                                                    >  1:7178543/1‑150 (MQ=255)
           aTCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATg                                                                                                                                   >  1:4984769/1‑150 (MQ=255)
                    cTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCaaa                                                                                                                          <  2:5391717/150‑1 (MQ=255)
                          cATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTg                                                                                                                    <  1:5859245/150‑1 (MQ=255)
                            tGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACTTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCATCTGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                                  <  2:6530439/150‑1 (MQ=255)
                            tGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                                  <  2:2242356/150‑1 (MQ=255)
                            tGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                                  <  1:1199651/150‑1 (MQ=255)
                            tGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                                  <  1:1474712/150‑1 (MQ=255)
                            tGAGCGGCGGCCGGACCGCGAGCGCGTCATCAGCGACGTGAAGAACCAGCTGCCGACCGAGGTGCTCGCCACAGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGCTCAGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                                  <  1:2206742/150‑1 (MQ=255)
                              aGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGt                                                                                                                >  2:5390731/1‑150 (MQ=255)
                                 ggcggcTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCa                                                                                                             <  2:1994022/150‑1 (MQ=255)
                                       tGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGa                                                                                                       >  1:2824014/1‑150 (MQ=255)
                                        ggACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGAt                                                                                                      <  1:4446361/150‑1 (MQ=255)
                                            cgcgAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTac                                                                                                  >  1:3181532/1‑150 (MQ=255)
                                                 gcgcTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgt                                                                                             >  2:3793077/1‑150 (MQ=255)
                                                  cgcTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtg                                                                                            >  2:2389523/1‑150 (MQ=255)
                                                   gcTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgc                                                                                           >  1:4535220/1‑150 (MQ=255)
                                                     ttCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGa                                                                                                                  >  2:467807/1‑125 (MQ=255)
                                                          caGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggt                                                                                    <  2:4442552/150‑1 (MQ=255)
                                                           aGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCgtgcggtg                                                                                   >  2:4154928/1‑150 (MQ=255)
                                                              gACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTgcgc                                                                                <  2:1682156/150‑1 (MQ=255)
                                                              gACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTgcgc                                                                                <  1:5020077/150‑1 (MQ=255)
                                                                 gTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTg                                                                             >  1:163120/1‑150 (MQ=255)
                                                                   gatgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCt                                                                           >  1:4388569/1‑150 (MQ=255)
                                                                   gatgaACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCt                                                                           >  1:6529229/1‑150 (MQ=255)
                                                                       aaCCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTg                                                                       >  1:3630859/1‑150 (MQ=255)
                                                                       aaCCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTg                                                                       <  1:1728653/150‑1 (MQ=255)
                                                                       aaCCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTg                                                                       >  1:2923049/1‑150 (MQ=255)
                                                                         ccAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGcc                                                                     <  2:6481639/150‑1 (MQ=255)
                                                                           aGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCgg                                                                   <  2:3363678/150‑1 (MQ=255)
                                                                            gCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGa                                                                  >  1:6693861/1‑150 (MQ=255)
                                                                            gCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGa                                                                  >  1:6693890/1‑150 (MQ=255)
                                                                                 aGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATcc                                                             <  2:5235174/150‑1 (MQ=255)
                                                                                  gAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCcg                                                            >  1:3141594/1‑150 (MQ=255)
                                                                                  gAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCcg                                                            >  2:3633942/1‑150 (MQ=255)
                                                                                  gAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCcg                                                            >  2:3824399/1‑150 (MQ=255)
                                                                                   aaCGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGCTCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCcgc                                                           <  1:3773754/150‑1 (MQ=255)
                                                                                   aaCGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCcgc                                                           <  1:1256757/150‑1 (MQ=255)
                                                                                   aaCGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGCCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCcgc                                                           >  1:971822/1‑150 (MQ=255)
                                                                                    aCGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATT‑GATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGa                                                         <  2:2965304/150‑1 (MQ=255)
                                                                                     cGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGa                                                         <  1:2705685/150‑1 (MQ=255)
                                                                                        gTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTg                                                      >  1:191880/1‑150 (MQ=255)
                                                                                        gTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTg                                                      >  2:4311501/1‑150 (MQ=255)
                                                                                         ttGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGc                                                     <  2:3479728/150‑1 (MQ=255)
                                                                                           gCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTa                                                   <  2:1572034/150‑1 (MQ=255)
                                                                                            cTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTac                                                  >  2:4179846/1‑150 (MQ=255)
                                                                                            cTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTac                                                  >  2:6205888/1‑150 (MQ=255)
                                                                                            cTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTac                                                  >  2:7206944/1‑150 (MQ=255)
                                                                                             tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGAcgtgc                                                                                           >  1:6973591/1‑108 (MQ=255)
                                                                                             tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTaca                                                 >  2:4382639/1‑150 (MQ=255)
                                                                                             tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTaca                                                 >  2:3221231/1‑150 (MQ=255)
                                                                                             tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTaca                                                 >  2:2903640/1‑150 (MQ=255)
                                                                                             tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTaca                                                 >  1:5510669/1‑150 (MQ=255)
                                                                                             tCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTaca                                                 >  1:5774665/1‑150 (MQ=255)
                                                                                              cGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTacac                                                >  2:867652/1‑150 (MQ=255)
                                                                                               gCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCttgtac                                               <  2:1206964/150‑6 (MQ=255)
                                                                                                   cTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCgcgc                                           <  2:3420358/150‑1 (MQ=255)
                                                                                                      gCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTgg                                        <  2:535744/150‑1 (MQ=255)
                                                                                                      gCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTgg                                        <  1:2054692/150‑1 (MQ=255)
                                                                                                        gTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGc                                      <  2:5774665/150‑1 (MQ=255)
                                                                                                         tGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCa                                     >  1:5034850/1‑150 (MQ=255)
                                                                                                             ggCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGt                                 <  2:3598420/150‑1 (MQ=255)
                                                                                                              gCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTg                                >  1:2602845/1‑150 (MQ=255)
                                                                                                              gCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTg                                >  2:5351244/1‑150 (MQ=255)
                                                                                                              gCCGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCCGGGCATCGTg                                <  2:6586430/150‑1 (MQ=255)
                                                                                                               ccGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGc                               <  1:4704815/150‑1 (MQ=255)
                                                                                                               ccGACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGc                               <  2:7023023/150‑1 (MQ=255)
                                                                                                                 gACATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGcac                             >  1:2974172/1‑150 (MQ=255)
                                                                                                                   cATCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCacac                           >  2:1898593/1‑150 (MQ=255)
                                                                                                                     tCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCt                         <  1:765422/150‑1 (MQ=255)
                                                                                                                     tCAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCt                         >  1:2213941/1‑150 (MQ=255)
                                                                                                                      cAGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCtt                        <  2:766615/150‑1 (MQ=255)
                                                                                                                       aGCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTtg                       >  2:4118302/1‑150 (MQ=255)
                                                                                                                        gCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTtgt                      <  2:7178543/150‑1 (MQ=255)
                                                                                                                        gCGGCCGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTtgt                      >  2:2768181/1‑150 (MQ=255)
                                                                                                                             cGGGCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTggcgg                 >  2:878542/1‑150 (MQ=255)
                                                                                                                              gggCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATcc                                                             <  2:3273284/105‑1 (MQ=255)
                                                                                                                              gggCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTggcggc                <  1:1062488/150‑1 (MQ=255)
                                                                                                                               ggCGAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCa               >  1:7227160/1‑150 (MQ=255)
                                                                                                                                  gAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCatat            >  1:1480390/1‑150 (MQ=255)
                                                                                                                                  gAAACATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCatat            >  2:4941910/1‑150 (MQ=255)
                                                                                                                                    aaCATATCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTc          <  1:3727258/150‑1 (MQ=255)
                                                                                                                                        tatCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCaa      <  1:3440462/150‑1 (MQ=255)
                                                                                                                                        tatCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCaa      <  1:5655598/150‑1 (MQ=255)
                                                                                                                                        tatCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCaa      <  1:6126682/150‑1 (MQ=255)
                                                                                                                                         atCTATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAg     <  1:4941910/150‑1 (MQ=255)
                                                                                                                                            tATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAg  >  2:5880914/1‑150 (MQ=255)
                                                                                                                                            tATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAg  <  2:673049/150‑1 (MQ=255)
                                                                                                                                            tATTGGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAg  >  1:673049/1‑150 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
AGTACAAGCAGATCGCCAAGCTGTTGCATGAGCGGCGGCTGGACCGCGAGCGCTTCATCAGCGACGTGATGAACCAGCTGCAGAACGAGTTGCTCGCCACTGGCGTGAAGGCCGACATCAGCGGCCGGGCGAAACATATCTATTCGATCTGGCGCAAGATGCAGCGCAAAGGCCTGGAGTTCAGCCAGATCTACGACGTGCGTGCGGTGCGCGTGCTGGTGCCGGAAATCCGCGACTGCTACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAG  >  NC_002947/1851618‑1851907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑