Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,656,409 C→T R446H (CGC→CAC)  tktA ← transketolase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,656,4090CT99.7% 1059.7 / ‑5.6 302R446H (CGC→CAC) tktAtransketolase A
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (172/129);  total (173/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 303 total aligned reads displayed.

TCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTGGCGTCATT  >  NC_002947/5656262‑5656554
                                                                                                                                                   |                                                                                                                                                 
  ggCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCAc                                                                                                                                               >  1:5739232/1‑150 (MQ=255)
   gCCGGGCGCCAGGTGTCCAGGTTCGGGGCGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACg                                                                                                                                              >  2:2369182/1‑150 (MQ=255)
     cGGGCGCCAGGTGTCCAGGTTCGGGGTGTTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGc                                                                                                                                            >  2:6180873/1‑150 (MQ=255)
     cGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGc                                                                                                                                            >  2:6601484/1‑150 (MQ=255)
     cGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGc                                                                                                                                            >  1:5470255/1‑150 (MQ=255)
          gcCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGc                                                                                                                                       <  1:3967/150‑1 (MQ=255)
          gcCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGc                                                                                                                                       >  2:246589/1‑150 (MQ=255)
           ccAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCACCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCg                                                                                                                                      <  2:5312455/150‑1 (MQ=255)
              ggTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGc                                                                                                                                   >  1:6936094/1‑150 (MQ=255)
               gtgtCCAGGTTCGGGGTGCTGCGCAGGCTAGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCg                                                                                                                                  >  1:1985662/1‑150 (MQ=255)
                  tCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTAt                                                                                                                               >  2:4706995/1‑150 (MQ=255)
                  tCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTAt                                                                                                                               >  2:3405917/1‑150 (MQ=255)
                    cAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTc                                                                                                                             >  1:5326775/1‑150 (MQ=255)
                    cAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTc                                                                                                                             <  2:5194670/150‑1 (MQ=255)
                      ggTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCa                                                                                                                           <  2:5176235/150‑1 (MQ=255)
                       gTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCAt                                                                                                                          >  2:4306633/1‑150 (MQ=255)
                              gTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATc                                                                                                                   <  1:628027/150‑1 (MQ=255)
                               tgctgcGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCa                                                                                                                  <  2:6817939/150‑1 (MQ=255)
                                  tgcGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGa                                                                                                               >  2:7256773/1‑150 (MQ=255)
                                  tgcGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGa                                                                                                               >  2:4972298/1‑150 (MQ=255)
                                   gcgcAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGaa                                                                                                              <  1:32019/150‑1 (MQ=255)
                                   gcgcAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGaa                                                                                                              <  2:3787784/150‑1 (MQ=255)
                                   gcgcAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGaa                                                                                                              <  1:1823143/150‑1 (MQ=255)
                                       aGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGaa                                                                                                              <  2:3506492/146‑1 (MQ=255)
                                       aGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTc                                                                                                          >  2:2177526/1‑150 (MQ=255)
                                       aGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTc                                                                                                          <  2:4242524/150‑1 (MQ=255)
                                           tGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCgcgc                                                                                                      <  1:4582650/150‑1 (MQ=255)
                                           tGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCgcgc                                                                                                      >  2:16828/1‑150 (MQ=255)
                                                aGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTaa                                                                                                 <  2:1236112/150‑1 (MQ=255)
                                                aGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTaa                                                                                                 <  1:3163015/150‑1 (MQ=255)
                                                aGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTaa                                                                                                 <  2:5532352/150‑1 (MQ=255)
                                                aGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTaa                                                                                                 <  1:6159698/150‑1 (MQ=255)
                                                aGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTaa                                                                                                 <  2:6030161/150‑1 (MQ=255)
                                                     ctCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCAc                                                                                            >  2:1066701/1‑150 (MQ=255)
                                                        gATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAg                                                                                         >  2:6915992/1‑150 (MQ=255)
                                                         aTCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAgg                                                                                        >  2:2308239/1‑150 (MQ=255)
                                                          tCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAAAATCAGGAAGGTCGCGCCGTAAGGCACCAGGc                                                                                       >  1:871032/1‑150 (MQ=255)
                                                                ggTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGt                                                                                 >  2:1253101/1‑150 (MQ=255)
                                                                   gCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCa                                                                              <  2:3212064/150‑1 (MQ=255)
                                                                    cGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAg                                                                             <  1:554483/150‑1 (MQ=255)
                                                                    cGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAg                                                                             <  1:2960739/150‑1 (MQ=255)
                                                                       cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGc                                                                          <  1:6037054/150‑1 (MQ=255)
                                                                       cGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGc                                                                          <  1:3889470/150‑1 (MQ=255)
                                                                          gCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCTCCAGGCCACCGTGCAGGGCAAc                                                                       <  2:11833/150‑1 (MQ=255)
                                                                          gCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAAc                                                                       <  2:1310079/150‑1 (MQ=255)
                                                                           ccGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACg                                                                      >  1:4783797/1‑150 (MQ=255)
                                                                            cGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGc                                                                     <  2:4865051/150‑1 (MQ=255)
                                                                              tCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCg                                                                   >  2:140721/1‑150 (MQ=255)
                                                                               cTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGt                                                                  <  1:7147538/150‑1 (MQ=255)
                                                                                ttCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGtt                                                                 >  2:4495196/1‑150 (MQ=255)
                                                                                 tCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTc                                                                >  1:7217551/1‑150 (MQ=255)
                                                                                 tCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTc                                                                <  1:6380769/150‑1 (MQ=255)
                                                                                    cccAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCatg                                                             >  2:1949925/1‑150 (MQ=255)
                                                                                     ccAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCatga                                                            >  1:2107627/1‑150 (MQ=255)
                                                                                           cGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCgg                                                      >  1:5137890/1‑150 (MQ=255)
                                                                                           cGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCgg                                                      >  1:2637657/1‑150 (MQ=255)
                                                                                           cGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCgg                                                      >  1:1780899/1‑150 (MQ=255)
                                                                                            gATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGt                                                     >  1:1042185/1‑150 (MQ=255)
                                                                                             aTGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACAACAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTc                                                    >  1:3409430/1‑150 (MQ=255)
                                                                                                gAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATg                                                 >  1:1168375/1‑150 (MQ=255)
                                                                                                 aGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGc                                                <  1:3703356/150‑1 (MQ=255)
                                                                                                     gTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGaa                                            >  1:2744871/1‑150 (MQ=255)
                                                                                                     gTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGaa                                            <  1:2331163/150‑1 (MQ=255)
                                                                                                       gggTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACt                                          >  2:5670043/1‑150 (MQ=255)
                                                                                                       gggTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACt                                          >  1:6659734/1‑150 (MQ=255)
                                                                                                        ggTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTc                                         <  1:2542227/150‑1 (MQ=255)
                                                                                                         gtgtACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTcg                                        <  1:5185454/150‑1 (MQ=255)
                                                                                                          tgtACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTcgc                                       >  1:5887208/1‑150 (MQ=255)
                                                                                                              cccGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCCACGCCGGACAGGATGGCGGTCATGCCGAACACGCGCAc                                   >  1:1683327/3‑150 (MQ=255)
                                                                                                               acGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACg                                  >  2:4827279/1‑150 (MQ=255)
                                                                                                               acGTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACg                                  >  2:1385965/1‑150 (MQ=255)
                                                                                                               acGTGGATCACGCGCTGCTTCATCAGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACg                                  >  2:2129469/1‑150 (MQ=255)
                                                                                                                 gTGGATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGcc                                >  2:775937/1‑150 (MQ=255)
                                                                                                                   ggATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGt                              >  2:6964767/1‑150 (MQ=255)
                                                                                                                    gATCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTa                             <  1:5674400/150‑1 (MQ=255)
                                                                                                                     aTCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAg                            <  2:5401564/150‑1 (MQ=255)
                                                                                                                      tCACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGa                           >  2:644746/1‑150 (MQ=255)
                                                                                                                       cACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGaa                          <  2:3982290/150‑1 (MQ=255)
                                                                                                                       cACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGaa                          >  2:3339998/1‑150 (MQ=255)
                                                                                                                       cACGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGaa                          <  1:4334828/150‑1 (MQ=255)
                                                                                                                        aCGCGCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAac                         <  2:5753282/150‑1 (MQ=255)
                                                                                                                        aCGCGCTGCGTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAac                         <  1:4992257/150‑1 (MQ=255)
                                                                                                                         cgcgCTGCTTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAaca                        <  2:6545810/150‑1 (MQ=255)
                                                                                                                            gctgctTCATCTGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATTCCGAACTCGCGCACGCCGTAGAACTCGt                     <  2:1873399/150‑1 (MQ=255)
                                                                                                                            gctgctTCATCAGGGCCGACATGTGAACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGt                     >  2:3941465/1‑150 (MQ=255)
                                                                                                                             ctgctTCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTa                    <  2:2745583/150‑1 (MQ=255)
                                                                                                                                 ttCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTg                >  1:6821308/1‑150 (MQ=255)
                                                                                                                                 ttCATCAGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTg                >  2:7270140/1‑150 (MQ=255)
                                                                                                                                    atcaGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCg             <  1:2411537/150‑1 (MQ=255)
                                                                                                                                    atcaGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCg             >  2:7049651/1‑150 (MQ=255)
                                                                                                                                     tcaGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGc            >  2:268473/1‑150 (MQ=255)
                                                                                                                                     tcaGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCAAGCCGAAATCGCGCACGCCGTATAACACGTAGTTGCCGc            >  1:311234/1‑150 (MQ=255)
                                                                                                                                       aGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTg          <  2:4084606/150‑1 (MQ=255)
                                                                                                                                       aGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTg          >  2:1486948/1‑150 (MQ=255)
                                                                                                                                       aGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTg          <  1:1550830/150‑1 (MQ=255)
                                                                                                                                       aGGGCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTg          <  1:1268341/150‑1 (MQ=255)
                                                                                                                                        gggCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTgg         >  1:4221683/1‑150 (MQ=255)
                                                                                                                                         ggCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTGGc        >  2:2990404/1‑150 (MQ=255)
                                                                                                                                          gCCGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTGGCg       >  1:5101534/1‑150 (MQ=255)
                                                                                                                                            cGACATGTGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTGGCGTc     >  2:5131272/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCCGCTGGCGTCATT  >  NC_002947/5656262‑5656554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑