Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,094,305	A→G	22.2%	T420A (ACC→GCC)	PP_3602 →	2,5‑dioxovalerate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,094,305	0	A	G	22.2%	30.5 / 4.6	18	T420A (ACC→GCC)	PP_3602	2,5‑dioxovalerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (5/9);  new base G (2/2);  total (7/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

GCCGATGCACGCCTGCTGGTCGAGTCCGACCCGCTGTTGCAGGAAGAAGTGTTTGGGCCCACCACCGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCATGGTGCACGGCGGCCCGTA  >  NC_002947/4094173‑4094450                                                                                                                                     |                                                                                                                                                  gCCGATGCACGCCTGCTGGTCGAGTCCGACCCGCTGTTGCAGGAAGAAGTGTTTGGGCCCACCACCGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGc                                                                                                                                  >  1:165139/1‑150 (MQ=255)                        gTCCGACCCGCTGTTGCAGGAAGAAGTGTTTGGGCCCACCACCGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGTCAGCTGGCcgcgca                                                                                                                                            >  1:140660/1‑115 (MQ=255)                        gTCCGACCCGCTGTTGCAGGAAGAAGTGTTTGGGCCCACCACCGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGTCAGCTGGCcgcgca                                                                                                                                            <  2:140660/117‑3 (MQ=255)                           cGACCCGCTGTTGCAGGAAGAAGTGTTTGGGCCCACCACCGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGc                                                                                                        <  2:391564/150‑1 (MQ=255)                                                 gtgtTTGGGCCCACCACCGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTggag                                                                                  <  2:401778/150‑1 (MQ=255)                                                            caccacCGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCgg                                                                       >  2:401881/1‑150 (MQ=255)                                                                               gTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGc                                                    <  1:401881/150‑1 (MQ=255)                                                                                       cAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGAc                                            <  2:425653/150‑1 (MQ=255)                                                                                             ccAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCctggctg                                                                                                 <  1:212436/91‑1 (MQ=255)                                                                                             ccAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCctggctg                                                                                                 >  2:212436/1‑91 (MQ=255)                                                                                             ccAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCgg                                                                       <  1:329150/117‑1 (MQ=255)                                                                                             ccAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGgtgt                                      <  2:99848/150‑1 (MQ=255)                                                                                             ccAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGgtgt                                      <  2:165139/150‑1 (MQ=255)                                                                                             ccAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCGGGAGGAGAAAGTCgg                                                                       >  2:329150/1‑117 (MQ=255)                                                                                                       gACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTggagga                                                                                >  2:200527/1‑98 (MQ=255)                                                                                                       gACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTggagga                                                                                <  1:200527/98‑1 (MQ=255)                                                                                                                       gtcgcgGTCAGCTGGCCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCATGGTGCAcg            >  1:77412/3‑150 (MQ=255)                                                                                                                                 gCTGGCCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCATGGTGCACGGCGGCCCGTa  <  2:77412/150‑1 (MQ=255)                                                                                                                                     |                                                                                                                                                  GCCGATGCACGCCTGCTGGTCGAGTCCGACCCGCTGTTGCAGGAAGAAGTGTTTGGGCCCACCACCGTGGCCGTAGAGGTGCAGGACAACGACCAACTGCGCGACGCGCTGCTCGGCCTGCGCGGCCAGCTGACCGCGACGCTGATCGGCGAAGCGGAGGACTTCGATGCCTTCGCCTGGCTGGTGCCGCTGCTGGAGGAGAAAGTCGGGCGAATCCTGGTCAATGGCTACCCGACCGGTGTCGAAGTCTGTGATGCCATGGTGCACGGCGGCCCGTA  >  NC_002947/4094173‑4094450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑