Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,212,535	A→G	20.0%	intergenic (+51/+43)	PP_4593 → / ← PP_4594	hypothetical protein/cystathionine gamma‑synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,212,535	0	A	G	20.0%	35.8 / 3.8	20	intergenic (+51/+43)	PP_4593/PP_4594	hypothetical protein/cystathionine gamma‑synthase
Reads supporting (aligned to +/- strand):  ref base A (9/7);  new base G (2/2);  total (11/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

GACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTC  >  NC_002947/5212401‑5212668                                                                                                                                        |                                                                                                                                      gacgaAGACGTCAACCGCCTGCGCAATGAAAGGTTGCTGATCAAGGACAGGATCGAACGGATCATTCATCCGGCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTACGACGGT‑GTAGGAGCgg                                                                                                                        <  2:205907/150‑1 (MQ=255)                cGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAg                                                                                                         <  1:52010/150‑1 (MQ=255)                                       gATCAAGGACAAGATCGAACGGATCATTCATCAGCCCAAATACTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGCCCCTCAGACAggcc                                                                                  >  2:107321/1‑147 (MQ=38)                                                cAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGAc                                                                                 <  1:196353/142‑1 (MQ=255)                                                cAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGAc                                                                                 >  2:196353/1‑142 (MQ=255)                                                   gATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCa                                                                      >  2:178749/1‑150 (MQ=255)                                                        aaCGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGcc                                                                 <  1:178749/150‑1 (MQ=255)                                                                           cAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGAGCTTTCAACGCCCCATCCAGGTCCTCGACCa                                              >  1:370300/1‑150 (MQ=255)                                                                               tCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTc                                          >  2:37905/1‑150 (MQ=255)                                                                                           tCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTc                              <  1:37905/150‑1 (MQ=255)                                                                                                   aTCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCCATCCCtc                      >  2:122368/1‑149 (MQ=255)                                                                                                    tCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAc                     >  1:78667/1‑150 (MQ=255)                                                                                                    tCCACCGACCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAANGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTAc                     <  1:107321/150‑1 (MQ=255)                                                                                                         cGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACa                >  2:471577/1‑150 (MQ=255)                                                                                                                  cgcgGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCgcagca       <  1:471577/150‑1 (MQ=255)                                                                                                                       gCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTtc  >  1:107145/1‑150 (MQ=255)                                                                                                                            cccGCTCCTAC‑GACGGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCgca          <  1:62636/137‑1 (MQ=39)                                                                                                                            cccGCTCCTAC‑GACGGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCgca          >  2:62636/1‑137 (MQ=255)                                                                                                                             ccGCTCCTAC‑GACGGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGt                                           >  2:110076/1‑103 (MQ=38)                                                                                                                             ccGCTCCTAC‑GACGGTGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGt                                           <  1:110076/103‑1 (MQ=38)                                                                                                                                        |                                                                                                                                      GACGAAGCCGTCAACCGCCTGCGCAAGGAAAGGTTGCTGATCAAGGACAAGATCGAACGGATCATTCATCCGCCCAAATCCTGACAGGCATCTATTGAATCCACCGGCCTCTTCGCGGGCAAGCCCGCTCCTAC‑ACCGTCGTAGGAGCGGGTTTACCCGCGAAAGGGCCTGCGGCCCTCACACAGCGACCAGCGCTTTCAACGCCCCATCCAGGTCCTCGACCAGGTCACGGTAATCCTCGATCCCTACCGACAACCGCAGCAGGTTC  >  NC_002947/5212401‑5212668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑