Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,269,306 C→A 10.0% G361C (GGC→TGC)  radA ← DNA repair enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,269,3060CA10.0% 117.6 / 2.8 40G361C (GGC→TGC) radADNA repair enzyme
Reads supporting (aligned to +/- strand):  ref base C (11/25);  new base A (2/2);  total (13/27)
Fisher's exact test for biased strand distribution p-value = 5.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

TGGCCACTGGGTACCGGGCGTACCTCGCCCGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTC  >  NC_002947/5269158‑5269441
                                                                                                                                                    |                                                                                                                                       
tGGCCACTGGGTACCGGGCGTACCTCGCCCGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCccgccg                                                                                                                                        >  2:105455/1‑150 (MQ=255)
    cACTGGGTACCGGGCGTACCTCGCCCGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCa                                                                                                                                    >  2:299320/1‑150 (MQ=255)
          gTACCGGGCGTACCTCGCCCGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTga                                                                                                                              <  1:462390/150‑1 (MQ=255)
           tACCGGGCGTACCTCGCCCGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTgag                                                                                                                             >  2:273056/1‑150 (MQ=255)
               gggCGTACCTCGCCCGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAac                                                                                                                         <  1:195617/150‑1 (MQ=255)
               gggCGTACCTCGCCCGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCAGACCACGTTGAGaaa                                                                                                                          <  2:135419/149‑1 (MQ=255)
               gggCGTACCTCGCCCGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCAGACCACGTTGAGaaa                                                                                                                          >  1:135419/1‑149 (MQ=255)
               gggCGTACCTCGCCCGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCAGACCACGTTGAGaaa                                                                                                                          >  1:135441/1‑149 (MQ=255)
               gggCGTACCTCGCCCGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCAGACCACGTTGAGaaa                                                                                                                          <  2:135441/149‑1 (MQ=255)
               gggCGTACCTCGCCAGACCATCCGATCTCGTCAAACACCAGCAAACCATGTGTGAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGTCAGGTCCGAAACCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAac                                                                                                                         <  2:225857/150‑1 (MQ=255)
                ggCGTACCTCGCCCGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTCCACCCCGCCGACCACGTTGAGAAaca                                                                                                                        >  2:84562/1‑150 (MQ=255)
                      ccTCGCCCGACAATCCGATCTCGCCAAGCACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCt                                                                                                                  <  2:305061/150‑1 (MQ=255)
                       cTCGCCCGACAATCCGATCTCGCCAAACACCAGAAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTg                                                                                                                 >  1:39165/1‑150 (MQ=255)
                       cTCGCCCGACAATCCGATATCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTg                                                                                                                 >  2:475067/1‑150 (MQ=255)
                             cGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATg                                                                                                           <  1:411103/150‑1 (MQ=255)
                                  aTCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCg                                                                                                      >  1:157256/1‑150 (MQ=255)
                                     cGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAAAAACACATCCTGGTCATGGGTCGGGa                                                                                                   <  1:190624/150‑1 (MQ=255)
                                         tccGCCAAACACCATCAAGCCATTGGCAAGTGGCCGGTTGCGTAAGCTCGTCATCACCGCCCCCAGCAGGGCCAGGTTCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATTCTGGTCATGGGTCGGGATAcc                                                                                               <  2:78559/148‑1 (MQ=255)
                                              cAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGTAACACATCCTGGTCATGGGTCGGGATACCGCCAt                                                                                          <  1:95752/150‑1 (MQ=255)
                                                    ccAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGt                                                                                    <  1:330592/150‑1 (MQ=255)
                                                    ccAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGt                                                                                    <  1:299320/150‑1 (MQ=255)
                                                     cAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGACGCCGCCCTCATCACCTTCACCCCGTCTTCCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTg                                                                                   <  1:305914/150‑1 (MQ=255)
                                                     cAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTg                                                                                   <  2:402660/150‑1 (MQ=255)
                                                       gCAAACCATGGGCCAGTGGCCGGTTGCGCATGCTCGACAGCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGtatc                                                                                 <  2:39165/150‑4 (MQ=255)
                                                             cATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTCCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCg                                                                           <  1:84562/150‑1 (MQ=255)
                                                              aTGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCCCACCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTAGGGATCACGGCATCGGTGTTGAGCACCGc                                                                          >  1:476222/1‑150 (MQ=255)
                                                                      gTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAacaaca                                                                  <  2:157256/150‑1 (MQ=255)
                                                                       tGGCCGGTTCCGCAACCTCTGCATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACAt                                                                 <  1:394102/150‑1 (MQ=255)
                                                                         gCCGGATGCGCAAGCAGGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATgg                                                               <  2:153004/150‑1 (MQ=255)
                                                                             gTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTg                                                                                   <  2:212278/126‑1 (MQ=255)
                                                                             gTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTg                                                                                   >  1:212278/1‑126 (MQ=255)
                                                                                         cGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTc                                               <  2:420973/150‑1 (MQ=255)
                                                                                             aTCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAgg                                           >  1:128052/1‑150 (MQ=255)
                                                                                                   gccgccAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAg                                     <  1:377129/150‑1 (MQ=255)
                                                                                                              ggCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACccgccg                          >  1:300159/1‑150 (MQ=255)
                                                                                                                      ccGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTgg                  <  1:475067/150‑1 (MQ=255)
                                                                                                                           gCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGa             <  2:128052/150‑1 (MQ=255)
                                                                                                                           gCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGa             <  2:297480/150‑1 (MQ=255)
                                                                                                                              gTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCcaa                                                                       >  2:280226/1‑89 (MQ=255)
                                                                                                                              gTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCcaa                                                                       <  1:280226/89‑1 (MQ=255)
                                                                                                                                      cACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTAtcgtc  <  1:105455/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                       
TGGCCACTGGGTACCGGGCGTACCTCGCCCGACAATCCGATCTCGCCAAACACCAGCAAACCATGGGCCAGTGGCCGGTTGCGCAAGCTCGACATCACCGCCGCCAGCAGGGCCAGGTCCGAAGCCGTTTCCAGCACCTTCACCCCGCCGACCACGTTGAGAAACACATCCTGGTCATGGGTCGGGATACCGCCATGGCGGTGGAGCACCGCCAACAACATGGCCAGACGGTTCTGGTCCAGGCCCAAGGTTACCCGCCGCGGGTTGGCCAGATGGCTATCGTC  >  NC_002947/5269158‑5269441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑