| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,559,994 | T→A | 11.1% | Q185L (CAG→CTG) | hisZ ← | ATP phosphoribosyltransferase regulatory subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,559,994 | 0 | T | A | 11.1% | 94.3 / 4.1 | 36 | Q185L (CAG→CTG) | hisZ | ATP phosphoribosyltransferase regulatory subunit |
| Reads supporting (aligned to +/- strand): ref base T (15/17); new base A (2/2); total (17/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
ACCGAGGCGCACGCGGGCTTCAGCCAGTACTTCCCGACCACCACACAGCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCGGCCAGTTGCAGCGTGGCGAGCATCAGGCTGATGACTTCGACATCGCTGGTAGGGC > NC_002947/5559846‑5560141 | aCCGAGGCGCACGCGGGCTTCAGCCAGTACTTCCCGACCACCACACAGCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAgctg < 2:171492/150‑1 (MQ=255) gcgcACGCGGGCTTCAGCCAGTACTTCCCGACCACCACACAGCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTc < 1:257838/150‑1 (MQ=255) gcgcACGCGGGCTTCAGCCAGTACTTCCCGACCACCACACAGCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTc < 1:295659/150‑1 (MQ=255) ttCAGCCAGTACTTCCCGACCACCACACAGCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCAGCTGTTCGAc < 2:464448/141‑1 (MQ=255) ttCAGCCAGTACTTCCCGACCACCACACAGCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCAGCTGTTCGAc > 1:464448/1‑141 (MQ=255) ccAGTACTTCCCGACCACCACACAGCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAgca < 2:194768/150‑3 (MQ=255) ccAGTACTTCCCGACCACCACACAGCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCAGCTGTTCg > 2:281712/1‑134 (MQ=255) ccAGTACTTCCCGACCACCACACAGCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCAGCTGTTCg < 1:281712/134‑1 (MQ=255) ttCCCGACCACCACACAGCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCggcggc > 1:233949/1‑150 (MQ=255) ccacACAGCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAgg > 2:355810/1‑150 (MQ=255) aGCTCGACCAGAGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCgg < 1:147817/150‑1 (MQ=255) gCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGt < 1:77573/150‑1 (MQ=255) tCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAg > 1:417641/1‑150 (MQ=255) tCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAg > 1:474993/1‑150 (MQ=255) gACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGa > 1:210712/1‑117 (MQ=255) gACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGa < 2:210712/117‑1 (MQ=255) cAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGcc > 2:361346/1‑150 (MQ=255) cAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGcc < 2:474993/150‑1 (MQ=255) cgcAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATgg < 2:233949/150‑1 (MQ=255) gcCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCAt < 1:224519/150‑1 (MQ=255) gcCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCAt < 1:435581/150‑1 (MQ=255) ccAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGCCCGCCTTGCGTTGCATTGCGTCGAACAGCTGATGGTCGCACGCGGCGCACGGTGCTGATGGTCTGGCCACGCCGCGGTAGATGCCGACATGGCCCAGGTCCAtgt < 1:8967/150‑1 (MQ=255) ggTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGACCTGGGCCAtgtcgg < 1:361346/150‑4 (MQ=255) tttCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATc > 2:93849/1‑150 (MQ=255) gcgcCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCGGCCAGTTGCa > 2:209083/1‑150 (MQ=255) ccTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGaa < 1:72080/123‑1 (MQ=255) ccTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGaa > 2:72080/1‑123 (MQ=255) aTCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCGGCCAGTTGCAGCGTGGCGAGCAt > 1:459361/1‑150 (MQ=255) aCAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCGGCCAGTTGCAGCGTGGCGAGCATCAgg < 1:283075/150‑1 (MQ=255) ccTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCGGCCAGTTGCAGCGTGGCGAGCATCAGGCtga > 1:295971/1‑150 (MQ=255) ttGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCGGCCAGTTGCa < 2:118115/127‑1 (MQ=255) ttGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCGGCCAGTTGCa > 1:118115/1‑127 (MQ=255) tgacGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCGGCCAGTTGCAGCGTGGCGAGCATCAGGCtgatg > 2:194825/4‑150 (MQ=255) gTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCGGCCAGTTGCAGCGTGGCGAGCATCAGGCTGATGACtt > 2:3634/1‑150 (MQ=255) ttGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCGGCCAGTTGCAGCGTGGCGAGCATCAGGCTGATGACTTc < 1:3634/150‑1 (MQ=255) ttGCATGGCGTCGAACAGCTGCTGTTCGACCGAGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAAGATCCGGCAGAGCGCCCAGTTGCAGCGTGGCGAGCATCAGGCTGATGACTTc < 1:209083/150‑1 (MQ=255) tCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCCGCCAGTTGCAGCGTGGCGAGCATCAGGCTGATGACTTCGACATCGCTg > 2:55478/1‑150 (MQ=255) gctgctGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCGGCCAGTTGCAGCGTGGCGAGCATCAGGCTGATGACTTCGACATCGCTGGTAGGGc > 1:208360/1‑150 (MQ=255) | ACCGAGGCGCACGCGGGCTTCAGCCAGTACTTCCCGACCACCACACAGCTCGACCAGGGCGCGCAGCATGCTGCCCAGGTCTTTCGGCAGGTCGGCCGTCAGCGCCTGCACTTCATCGACAGCCTTGCGTTGCATGGCGTCGAACAGCTGCTGTTCGACCGCGCCGGACAGACCGGCGGCACGGGCCAGGCCGCGGTAGATGCCGACATGGCCCAGGTCCATGTGAACATCCGGCACATCGGCCAGTTGCAGCGTGGCGAGCATCAGGCTGATGACTTCGACATCGCTGGTAGGGC > NC_002947/5559846‑5560141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |