| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 197,551 | C→G | 16.7% | T1019T (ACC→ACG) | PP_0168 → | putative surface adhesion protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 197,551 | 0 | C | G | 16.7% | 80.9 / 11.7 | 36 | T1019T (ACC→ACG) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base C (16/14); new base G (3/3); total (19/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
ACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACC > NC_002947/197405‑197683 | aCCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCAtt > 2:416698/1‑150 (MQ=38)aCCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCAtt > 1:360375/1‑150 (MQ=37) cACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTAc < 1:168445/150‑1 (MQ=38) gATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGt > 1:476454/1‑150 (MQ=38) aaCGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGGCCCTGGCCATCGTCCAAACCAGTACCGTTgta > 2:351638/1‑148 (MQ=11) tCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGaa < 1:51517/140‑1 (MQ=40) tCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGaa > 2:51517/1‑140 (MQ=39) ccaccaCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAg > 2:340996/1‑149 (MQ=39) ccaccaCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAg < 1:340996/149‑1 (MQ=39) accaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGc > 2:51716/1‑150 (MQ=38) gACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCTTGGGCAATCTTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAATCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCGGCGTCGa > 1:88701/1‑150 (MQ=11) ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGAttt > 1:418499/1‑150 (MQ=39) cTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCCGACGGCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTc < 2:463099/150‑1 (MQ=255) gAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGAcccc < 1:477300/150‑1 (MQ=38) gTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGaa < 1:351638/150‑1 (MQ=39) cggcgTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTAcaacaa < 2:476454/150‑1 (MQ=39) ggcgTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTg > 2:465773/1‑88 (MQ=40) ggcgTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTg < 1:465773/88‑1 (MQ=40) cgTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACgg > 2:62754/1‑150 (MQ=38) tGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCa < 2:286858/150‑1 (MQ=18) ccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCAt < 1:51716/150‑1 (MQ=17) ccTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTT‑‑‑GCCGTAACAGTCGTGCACTTCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGCTCGACTGTCAGCGTCACCAt > 2:142114/1‑150 (MQ=11) tGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCg > 1:128307/1‑150 (MQ=38) tGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCg > 1:423440/1‑150 (MQ=17) gAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCga < 1:62754/150‑1 (MQ=39) cAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGc > 2:459625/1‑150 (MQ=39) aGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCg < 2:373933/150‑1 (MQ=11) aGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCg < 1:416698/150‑1 (MQ=17) gACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCgg < 2:418499/150‑1 (MQ=39) gaCCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGCAGCCGGCG‑AAAC‑‑GCAGGTCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACACCGGTTCGACTGTCAGCGTAACAATCGAGAACGCCACAGGCGGTATTTTCGa < 2:399172/149‑1 (MQ=11) ccTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAc > 2:282579/1‑150 (MQ=12) cTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAcc > 1:48356/1‑150 (MQ=12) tGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAc < 2:405224/148‑1 (MQ=32) tGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGAc > 1:405224/1‑148 (MQ=32) gCCAAACGATTACCGTTGAAGCCGGCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa > 1:265389/1‑130 (MQ=255) gCCAAACGATTACCGTTGAAGCCGGCA‑AAAC‑‑CCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGa < 2:265389/130‑1 (MQ=255) | ACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGATCCTGAGCAATCCTGCCCAGACGCCGGTAACCGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCA‑AGAC‑‑CCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACC > NC_002947/197405‑197683 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |