Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	321,309	G→A	18.2%	W234* (TGG→TAG)	aguA →	agmatine deiminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	321,309	0	G	A	18.2%	61.6 / 8.8	33	W234* (TGG→TAG)	aguA	agmatine deiminase
Reads supporting (aligned to +/- strand):  ref base G (13/14);  new base A (3/3);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.66e-01

GCAGATTGAAGCGGTGCTGCGCGATCACTTGGCGGTCGACACCGTGGTATGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGA  >  NC_002947/321169‑321450                                                                                                                                             |                                                                                                                                              gCAGATTGAAGCGGTGCTGCGCGATCACTTGGCGGTCGACACCGTGGTATGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCgatga                                                                                                                                      <  1:1637/150‑1 (MQ=255)     aTTGAAGCGGTGCTGCGCGATCACTTGGCGGTCGACACCGTGGTATGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTcc                                                                                                                                  >  2:211335/1‑150 (MQ=255)     aTTGAAGCGGTGCTGCGCGATCACTTGGCGGTCGACACCGTGGTATGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTcc                                                                                                                                  >  1:368489/1‑150 (MQ=255)                  tgcGCGATCACTTGGCGGTCGACACCGTGGTATGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACt                                                                                                                     >  2:152937/1‑150 (MQ=255)                    cgcgATCACTTGGCGGTCGACACCGTGGTATGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTAt                                                                                                                   <  2:195367/150‑1 (MQ=255)                       gATCACTTGGCGGTCGACACCGTGGTATGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATgcg                                                                                                                >  1:415865/1‑150 (MQ=255)                                  ggTCGACACCGTGGTATGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCAcgc                                                                                                     <  1:211335/150‑1 (MQ=255)                                    tCGACACCGTGGTATGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCgg                                                                                                   <  2:274836/150‑1 (MQ=255)                                     cgccacCGAGGGATGGCTGCCCGCAGGGACATTCAACGACTGAACGCCCGTTGACGACGACAACTCCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGc                                                                                                  <  2:30259/147‑1 (MQ=255)                                         acCGTGGTATGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATg                                                                                              <  2:75910/150‑1 (MQ=255)                                          ccGTGGTATGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACATGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATgg                                                                                             >  2:21461/1‑150 (MQ=255)                                            ggggTATGGCTGCCCGCGTGCCTGTACAACGACGAAACTGCCGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCACCTATGCGCGCTCCCACGCGGCCATGGAt                                                                                           <  2:234804/148‑1 (MQ=255)                                                  tGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTg                                                                                     >  1:475406/1‑150 (MQ=255)                                                         ccGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGAACGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATTTGCGAAAGAaca                                                                              >  2:229834/1‑150 (MQ=255)                                                                       aaCGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCAGGGATGTGCTGAAGAACACCCGCGACGCCCAg                                                                >  2:196270/1‑150 (MQ=255)                                                                              aaaCTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCcgcg                                                         >  2:283751/1‑150 (MQ=255)                                                                               aaCTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGa                                                        <  2:171698/150‑1 (MQ=255)                                                                                  tGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTAGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGtt                                                     >  2:277851/1‑150 (MQ=255)                                                                                  tGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTAGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGtt                                                     >  2:277801/1‑150 (MQ=255)                                                                                  tGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTAGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGtt                                                     >  2:31877/1‑150 (MQ=255)                                                                                          aCGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGc                                             >  2:370627/1‑150 (MQ=255)                                                                                              cGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACaa                                         <  1:152937/150‑1 (MQ=255)                                                                                               gACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAg                                        >  2:112527/1‑150 (MQ=255)                                                                                                aCAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGa                                       <  1:283751/150‑1 (MQ=255)                                                                                                   aCTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGc                                    <  1:298161/150‑1 (MQ=255)                                                                                                   aCTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGc                                    <  1:112527/150‑1 (MQ=255)                                                                                                        tgctgTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATc                               >  1:308208/1‑150 (MQ=255)                                                                                                           tgTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCAGACATGGATGTGCTGAAGAACGCTTGCGTCGCCAGGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCg                            <  1:9295/150‑1 (MQ=255)                                                                                                             ttACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGAGAAGCCAAGGGCAGGGAAAGAATCTTTCAGAAGATGCCGATCCCggg                          >  2:129667/1‑150 (MQ=255)                                                                                                                                   ttACTGGCCTAGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGaa    <  1:31877/150‑1 (MQ=255)                                                                                                                                   ttACTGGCCTAGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGaa    <  1:277801/150‑1 (MQ=255)                                                                                                                                   ttACTGGCCTAGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGCTTGCCACTGCCGaa    <  1:277851/150‑1 (MQ=255)                                                                                                                                     aCTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAaga  <  1:370627/150‑1 (MQ=255)                                                                                                                                             |                                                                                                                                              GCAGATTGAAGCGGTGCTGCGCGATCACTTGGCGGTCGACACCGTGGTATGGCTGCCCGATGGCCTGTACAACGACGAAACTGACGGTCACGTCGACAACTTCTGCTGTTACGTGCGCCCGGGTGAAGTGTTACTGGCCTGGACCGATGATTCCAACGACCCCAACTATGCGCGCTGCCACGCGGCCATGGATGTGCTGAAGAACACCCGCGACGCCAAGGGCCGCGAGTTCATCGTGCACAAGATGCCGATCCCGGGCCCGCTGTTTGCCACTGCCGAAGA  >  NC_002947/321169‑321450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑