| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,204,422 | G→T | 18.2% | intergenic (+114/+46) | gspN → / ← PP_1056 | type II secretion pathway protein GspN/iron‑chelator utilization protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,204,422 | 0 | G | T | 18.2% | 48.9 / 3.6 | 22 | intergenic (+114/+46) | gspN/PP_1056 | type II secretion pathway protein GspN/iron‑chelator utilization protein |
| Reads supporting (aligned to +/- strand): ref base G (9/9); new base T (2/2); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01 | |||||||||||
CTGGCGGGGCAGCGAGACCTTCGTTGGTAAGCAACAACGTGGCGCGGCCCCCTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAATCACGATCACTCGTCGTCCGCAGTCCCGTTGGCCCGCCAGTAGGCTGCAGCCTTTAGCGCATCTTCCGGCACCCCTTTCTCCAGCAACAACGCCTTGGCCTGGCGAGTC > NC_002947/1204279‑1204570 | cTGGCGGGGCAGCGAGACCTTCGTTGGTAAGCAACAACGTGGCGCGGCCCCCTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCttt < 1:63420/150‑1 (MQ=255) gagaCCTTCGTTGGTAAGCAACAACGTGGCGCGGCCCCCTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGc < 1:202926/150‑1 (MQ=255) aCCTTCGTTGGTAAGCAACAACGTGGCGCGGCCCCCTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTgcgc < 2:377133/131‑1 (MQ=255) aCCTTCGTTGGTAAGCAACAACGTGGCGCGGCCCCCTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTgcgc > 1:377133/1‑131 (MQ=255) caacaaCGTGGCGCGGCCCCCTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGc > 1:89942/1‑150 (MQ=255) acaacGTGGCGCGGCCCCCTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGaa > 1:196940/1‑133 (MQ=255) acaacGTGGCGCGGCCCCCTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGaa < 2:196940/133‑1 (MQ=255) caacGTGGCGCGGCCCCCTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTGGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAAt > 2:284392/1‑150 (MQ=255) tGGCGCGGCCCCCTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAATCACGa < 1:27225/150‑1 (MQ=255) ccccTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAATCACGATCACtcgtc < 2:240413/150‑1 (MQ=255) ccTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGCAGAAACCGCGAATCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAATCACGATCACTcgtcgt > 1:428516/1‑150 (MQ=255) gATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTTCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGc < 1:221849/125‑1 (MQ=255) gATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTTCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGc < 1:221839/125‑1 (MQ=255) gATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTTCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGc > 2:221839/1‑125 (MQ=255) gATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTTCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGc > 2:221849/1‑125 (MQ=255) gCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTgg < 2:144215/95‑1 (MQ=255) gCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTgg > 1:144215/1‑95 (MQ=255) tGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAATCACGATCACTCGTCGTCCGCAGTCCCGTTGGCCCGCCAGTAGGCTGCAg > 2:374107/1‑150 (MQ=255) aGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAATCACGATCACTCGTCGTCCGCAGTCCCGTTGGCCCGCCAGTAGGCTGCAGcc > 1:333013/1‑150 (MQ=255) ttGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAATCACGATCACTCGTCGTCCGCAGTCCCGTTGGCCCGCCAGTAGGCTGCAGCCTTTAgc < 2:333013/150‑1 (MQ=255) tCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAATCACGATCACTCGTCGTCCGCAGTCCCGTTGGCCCGCCAGTAGGCTGCAGCCTTTAGCGCATCTTCCGGCACCCCtt > 2:126217/1‑150 (MQ=255) ttATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAATCACGATCACTCGTCGTCCGCAGTCCCGTTGGCCCGCCAGTAGGCTGCAGCCTTTAGCGCATCTTCCGGCACCCCTTtc > 1:407637/1‑150 (MQ=255) cgcgAAGCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAATCACGATCACTCGTCGTCCGCAGTCCCGTTGGCCCGCCAGTAGGCTGCAGCCTTTAGCGCATCTTCCGGCACCCCTTTCTCCAGCAACAACGCCTTg < 2:407637/150‑1 (MQ=255) tGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAATCACGATCACTCGTCGTCCGCAGTCCCGTTGGCCCGCCAGTAGGCTGCAGCCTTTAGCGCATCTTCCGGCACCCCTTTCTCCAGCAACAACGCCTTGGCCTGGCGAGTc < 1:284392/150‑1 (MQ=255) | CTGGCGGGGCAGCGAGACCTTCGTTGGTAAGCAACAACGTGGCGCGGCCCCCTGTAGATAGGCTGTTGGCGGTCGAGCTTTTCTGAGGTGCTTGGGGCTGAACCGCTTGGTCTTATCAAATGGTTTAAACCGCGAAGCAGGCTGCGCTTTGCGTGGCACCGGCGAAGACGGCAGATCAGGCAATCACGATCACTCGTCGTCCGCAGTCCCGTTGGCCCGCCAGTAGGCTGCAGCCTTTAGCGCATCTTCCGGCACCCCTTTCTCCAGCAACAACGCCTTGGCCTGGCGAGTC > NC_002947/1204279‑1204570 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |