| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,356,411 | G→C | 11.7% | G94A (GGC→GCC) | PP_1181 → | two‑component system response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,356,411 | 0 | G | C | 11.7% | 88.2 / 3.6 | 32 | G94A (GGC→GCC) | PP_1181 | two‑component system response regulator |
| Reads supporting (aligned to +/- strand): ref base G (13/15); new base C (2/2); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
ATCGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATC > NC_002947/1356266‑1356550 | atcGACAGCCAGCCCGACCTGGTCATCCTCGACCTGATGCGGCCCCGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCATCCAGGACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTTCAGGCCCTg < 2:124485/150‑1 (MQ=255) gACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCGTCATGCTGACCACGTGAAGTGACGAACCGGACCAGCGCCAGGGCCTGGAc > 2:178063/1‑150 (MQ=255) agccagccCGACCTGGTGATCCTCGACCTTATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTg > 1:91032/1‑150 (MQ=255) ccagccCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTggg > 1:457018/1‑150 (MQ=255) cagccCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGgc < 1:439175/150‑1 (MQ=255) aCCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAAc < 1:307699/150‑1 (MQ=255) tGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTgc < 1:178063/150‑1 (MQ=255) gCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTgcg > 1:193097/1‑150 (MQ=255) gCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGACGGGACTCCCGAACGTTTTTCATGCTGATCCTCATCCGCTCCGCGCGCAGTGATGAACTGGACCAGGTCCATGGCCTGGACCTGGGTGCCGACGACTACGTCTGCAAACCCGTgcg > 1:134280/1‑150 (MQ=255) cccGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCa < 2:91032/150‑1 (MQ=255) gCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCt < 2:296449/150‑1 (MQ=255) ggACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTtgct > 2:51035/1‑150 (MQ=255) ggCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGctgctg > 1:250930/1‑150 (MQ=255) ccTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTtgct < 2:67273/144‑1 (MQ=255) ccTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTtgct > 1:67273/1‑144 (MQ=255) tGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCAc < 1:68219/150‑1 (MQ=255) gcAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGcgcc > 2:257612/1‑150 (MQ=255) gcAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGcgcc > 1:16901/1‑150 (MQ=255) gCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCa < 2:134280/150‑1 (MQ=255) cAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGc < 1:361109/150‑1 (MQ=255) tACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGATCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGaa > 2:69604/1‑150 (MQ=255) aCCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGaaa < 2:457018/150‑1 (MQ=255) cAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCACCCACGCTTGCTGCTGGCACGTATCTATGCCCTGCTGCGCCGCAGCGAAAcc < 1:257612/150‑1 (MQ=255) gATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAg > 2:316994/1‑150 (MQ=255) gTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCg < 1:416532/150‑1 (MQ=255) tgatgaACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCGCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCgg > 2:9380/1‑150 (MQ=255) tGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCGCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGc < 1:9380/150‑1 (MQ=255) cAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATc < 1:69604/150‑1 (MQ=255) cAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATc < 1:224221/150‑1 (MQ=255) cAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGctgctg > 1:47783/1‑69 (MQ=255) cAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGctgctg < 2:47783/69‑1 (MQ=255) cAGGTCCAGGCCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATc > 2:150406/1‑150 (MQ=255) | ATCGACAGCCAGCCCGACCTGGTGATCCTCGACCTGATGCTCCCCGGCGAGGACGGCCTGAGCATCTGCCGCCGGGTGCGCAGCCAGTACCAGGGGCCGATCCTCATGCTCACCGCGCGCAGTGATGAACTGGACCAGGTCCAGGGCCTGGACCTGGGCGCCGACGACTACGTCTGCAAACCCGTGCGCCCACGCTTGCTGCTGGCACGTATCCAGGCCCTGCTGCGCCGCAGCGAAACCGTGGACAGCAAGCGCCAGGACCTGGCCTTCGGTGCGCTGCGTATC > NC_002947/1356266‑1356550 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |