| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,852,053 | G→T | 37.5% | W366L (TGG→TTG) | relA → | ATP:GTP 3'‑pyrophosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,852,053 | 0 | G | T | 37.5% | 10.4 / 10.4 | 16 | W366L (TGG→TTG) | relA | ATP:GTP 3'‑pyrophosphotransferase |
| Reads supporting (aligned to +/- strand): ref base G (5/5); new base T (3/3); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01 | |||||||||||
GACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCT > NC_002947/1851914‑1852195 | gacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAg < 2:44486/150‑1 (MQ=255) aCATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTCGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCa < 2:61153/150‑1 (MQ=255) gCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGAc > 2:238176/1‑150 (MQ=255) ccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGc > 1:241576/1‑150 (MQ=255) tACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCAc > 1:315145/1‑150 (MQ=255) gTGATGGAGGTGCAGATCCGGACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTgg > 1:333698/1‑150 (MQ=255) gAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCa > 1:437249/1‑146 (MQ=255) gAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCa < 2:437249/146‑1 (MQ=255) gCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCt < 2:242850/150‑1 (MQ=255) gATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTggg < 2:299250/150‑1 (MQ=255) ccACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCgg > 2:298964/1‑150 (MQ=255) cACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGt > 2:302190/1‑150 (MQ=255) cACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtggcctggcc < 2:147998/150‑1 (MQ=255) ggCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGAc < 1:302190/150‑1 (MQ=255) cgcCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGcc > 1:180786/1‑150 (MQ=255) gcCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCt < 2:333698/150‑1 (MQ=255) | GACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCT > NC_002947/1851914‑1852195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |