| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,112,781 | G→C | 19.1% | H516Q (CAC→CAG) ‡ | PP_1882 ← | membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,112,781 | 0 | G | C | 19.1% | 40.7 / 7.2 | 21 | H516Q (CAC→CAG) ‡ | PP_1882 | membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (11/6); new base C (2/2); total (13/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-01 | |||||||||||
TGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGG > NC_002947/2112648‑2112919 | tGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAggg > 1:63316/1‑150 (MQ=255) tggtggtTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAAGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGa > 1:198983/1‑150 (MQ=255) ccAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCg < 2:168421/150‑1 (MQ=255) cctcctGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTtgc > 2:449336/1‑150 (MQ=255) gCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCg > 2:175759/1‑108 (MQ=255) gCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCg < 1:175759/108‑1 (MQ=255) gCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGt > 2:362387/1‑150 (MQ=255) gCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCg > 2:129091/1‑150 (MQ=255) gCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCg > 2:363445/1‑150 (MQ=255) gCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCg > 1:223792/1‑150 (MQ=255) cgcATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtgagt < 1:362387/150‑1 (MQ=255) aTCGCGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGTTTCAGGGTTTCACTGTGCTCGACAACCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCtgagtgag > 2:438157/1‑150 (MQ=255) ggTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAg < 1:363445/150‑1 (MQ=255) gTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAgg < 2:63316/150‑1 (MQ=255) gTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTg < 2:198983/150‑1 (MQ=255) cctgacgcATGCGCACGATCCGGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGgc > 1:386946‑M1/9‑93 (MQ=255) cctgacgcATGCGCACGATCCGGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGgc < 2:386946‑M1/85‑1 (MQ=255) ctgacgcATGCGCACGATCCGGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGtt < 2:205950‑M1/72‑1 (MQ=255) ctgacgcATGCGCACGATCCGGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGtt > 1:205950‑M1/8‑79 (MQ=255) aGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGGGAACAGGGGCTTGCCCCCGGCCGCTGCTc > 2:101957/1‑150 (MQ=255) TGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCggg > 1:387345‑M1/1‑150 (MQ=255) | TGAACTGGTACTGGGTGGTGGTTTCCAGCTTGTTGCGTTTCACCACCTCCTGATAGCGACGGCCGTGCCGCAGGCTGGACTTGGGCGCATCGAGGTAATAGTAAATTTTCTCTTCCAGGGGATGCGCCGGATCGTGCGCATGCGTCAGGGTTTCACTGTGCTCGACAATCATCGGGTTGAGGGTGATGGCGTTGCCTGCCAGTGGCGCCAGCGCCTTGTAGCGGTAGTGCTGAGTGAGAACAGGGGCTTGCCCCCGGCCGCTGCTCGCCGGG > NC_002947/2112648‑2112919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |