| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,478,892 | T→G | 23.5% | intergenic (+102/+343) | PP_2172 → / ← PP_2173 | hypothetical protein/AraC family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,478,892 | 0 | T | G | 23.5% | 26.6 / 6.4 | 17 | intergenic (+102/+343) | PP_2172/PP_2173 | hypothetical protein/AraC family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (7/6); new base G (2/2); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01 | |||||||||||
GCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCAAGAAGGT > NC_002947/2478747‑2479023 | gCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCg < 2:426909/150‑1 (MQ=255) cttcttCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGgaga < 2:335856/150‑1 (MQ=255) aTGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCAcga < 2:252768/150‑1 (MQ=255) gatgatTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGt > 1:94501/1‑150 (MQ=255) tttGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAAAGCTCAGCGCTCGCAGGgtgtggg < 1:388661/117‑4 (MQ=37) tttGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAAAGCTCAGCGCTCGCAGGgtgtggg > 2:388661/1‑114 (MQ=37) aTGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGgatcg > 1:152434/1‑150 (MQ=255) ggCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTaa < 1:389645/150‑1 (MQ=255) gCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGAtctc > 2:61735/1‑144 (MQ=255) gCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGAtctc < 1:61735/144‑1 (MQ=255) gagCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGc > 2:45215/1‑150 (MQ=255) tCCGCGTAGGCCGTGCTAAGTGAACGGGTCCCCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATgg > 1:65039/1‑150 (MQ=255) gCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAAAGCTCAGCGCTCGCAGGgtgtg < 1:469021/62‑2 (MQ=14) gCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAAAGCTCAGCGCTCGCAGGgtgtg > 2:469021/1‑61 (MQ=14) aCGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCgg < 1:441061/150‑1 (MQ=255) ccccACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCaa > 1:424548/1‑150 (MQ=255) acCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCAAGAAGGt > 1:170936/1‑150 (MQ=255) | GCCTCAGCAACGCTGTCGGGTCTTCTTCAAAAAATGATCGATGATTTGACCGCATCCATGCAGTACCGGCTGTAGCGTGCACGAGAGCTCCGCGTAGGCCGTGCTAATTGAACGGGTTACCCCCCACACCCTGCGAGCGCTGAGCTTTCGCAGGGTGTTTTGTTTTGGAGACCATCATCACGAGCGAGTCAGCACGTGGCGGGATCGATCTCGGTAACCACAGTTACCTCCGGCATGGCCAAGGTACGTTGGTCCAAGCGGTGTTTCGCAAGAAGGT > NC_002947/2478747‑2479023 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |