BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,656,850	C→A	G299V (GGT→GTT)	tktA ←	transketolase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,656,850	0	C	A	99.0%	319.9 / ‑4.2	104	G299V (GGT→GTT)	tktA	transketolase A
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (56/47); total (56/48)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.64e-01

Only 100 of 104 total aligned reads displayed.

CACTTCGTTGATGTAGGCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCACCGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCTTCCTTGCCCTGCTTGTTCG  >  NC_002947/5656705‑5656994

cACTTCGTTGATGTAGGCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAAcggc                                                                                                                                              <  1:1915869/150‑1 (MQ=255)

 aCTTCGTTGATGTAGGCCTGTGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCACcggcg                                                                                                                                             <  2:1285303/150‑1 (MQ=255)

 aCTTCGTTGATGTAGGCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAAcggcg                                                                                                                                             >  1:1399587/1‑150 (MQ=255)

  cTTCGTTGATGTAGGCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAAcggcgg                                                                                                                                            >  1:373581/1‑150 (MQ=255)

    tCGTTGATGTAGGCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAAcggc                                                                                                                                              <  2:933519/146‑1 (MQ=255)

     cGTTGATGTAGGCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCt                                                                                                                                         >  1:125135/1‑150 (MQ=255)

      gTTGCTGAAGGCCTGGGCCTTTTCGTTGAAGTCGGCCGGCAGCGCGCCGCTGGCACGGCGCTTGAACTGGGCAGCCAGCTCCGGGTAGGCGTTGGGCTGGGGAACGAAGCCCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGTCtt                                                                                                                                        <  2:312788/150‑1 (MQ=255)

      gTTGATGTAGGCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCtt                                                                                                                                        >  1:1615145/1‑150 (MQ=255)

      gTTGATGTAGGCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAAACTTGGCTACGGCGGcccg                                                                                                                                        >  2:1807444/1‑148 (MQ=255)

        tGATGTAGGCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTgg                                                                                                                                      <  2:1463955/150‑1 (MQ=255)

             tAGGCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATcc                                                                                                                                 <  1:504806/150‑1 (MQ=255)

             tAGGCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATcc                                                                                                                                 <  2:2118070/150‑1 (MQ=255)

               ggCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCa                                                                                                                               >  1:1680714/1‑150 (MQ=255)

                  cTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTc                                                                                                                            >  1:1419186/1‑150 (MQ=255)

                  cTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTc                                                                                                                            >  1:203645/1‑150 (MQ=255)

                  cTGAGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTc                                                                                                                            >  2:494389/1‑150 (MQ=255)

                    gggCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCCGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCgg                                                                                                                          >  2:396498/1‑150 (MQ=255)

                     ggCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGc                                                                                                                         <  2:1691502/150‑1 (MQ=255)

                     ggCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACCCGGCTTCAACGTTAGCAACGGCGTCCTTGGCATCCCACTCGGc                                                                                                                         >  2:810330/1‑150 (MQ=255)

                      gCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTAGTTCCACTCGGCTGCAACCTGGGCAACGGCGGCCTTGGCATCCCACTAggag                                                                                                                        >  2:471672/1‑148 (MQ=255)

                        cTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTa                                                                                                                      <  2:1417673/150‑1 (MQ=255)

                           ttCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGAt                                                                                                                   >  1:411850/1‑150 (MQ=255)

                              gCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTc                                                                                                                <  1:1867215/150‑1 (MQ=255)

                                tGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAAcg                                                                                                                                                <  1:2205809/116‑1 (MQ=255)

                                   aGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCg                                                                                                           <  2:662156/150‑1 (MQ=255)

                                         ccggcAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGAttt                                                                                                     <  2:1419186/150‑1 (MQ=255)

                                          cggcAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTc                                                                                                    <  2:373581/150‑1 (MQ=255)

                                                 tCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCGACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGACTTCGAACggg                                                                                             >  2:1539311/1‑150 (MQ=255)

                                                 tCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCg                                                                                                           <  1:221966/136‑1 (MQ=255)

                                                 tCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCg                                                                                                           >  2:221966/1‑136 (MQ=255)

                                                 tCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACggg                                                                                             >  1:1488769/1‑150 (MQ=255)

                                                  cgccgcTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGc                                                                                            <  2:1233652/150‑1 (MQ=255)

                                                     cgcTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGt                                                                                         <  2:1399587/150‑1 (MQ=255)

                                                      gcTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTg                                                                                        >  1:1432490/1‑150 (MQ=255)

                                                         ggCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGtt                                                                                     <  1:1147517/150‑1 (MQ=255)

                                                         ggCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTAGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGtt                                                                                     <  2:1615145/150‑1 (MQ=255)

                                                          gCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTc                                                                                    <  2:125135/150‑1 (MQ=255)

                                                          gCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTc                                                                                    <  1:1604126/150‑1 (MQ=255)

                                                            aCGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAGCCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGaa                                                                                                 >  1:1811136/1‑135 (MQ=255)

                                                            aCGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAGCCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGaa                                                                                                 <  2:1811136/135‑1 (MQ=255)

                                                              ggCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGt                                                                                >  1:348579/1‑150 (MQ=255)

                                                              ggCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGt                                                                                >  2:132821/1‑150 (MQ=255)

                                                              ggCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGt                                                                                >  2:52924/1‑150 (MQ=255)

                                                              ggCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGt                                                                                >  1:1622209/1‑150 (MQ=255)

                                                              ggCGCTTGAACTCGGCAGCCAGCTCCGGATAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCCTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCTTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGt                                                                                >  2:384112/1‑150 (MQ=255)

                                                                    tGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAggg                                                                          <  1:813761/150‑1 (MQ=255)

                                                                         tcagCAGCCAGGTCGGTGTTGGCGTTGTCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTgg                                                                     >  2:1609124/4‑150 (MQ=255)

                                                                         tCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTgg                                                                     >  2:621955/1‑150 (MQ=255)

                                                                            gCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCCCTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCgg                                                                  <  1:471672/150‑1 (MQ=255)

                                                                            gCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCgg                                                                  >  2:2033721/1‑150 (MQ=255)

                                                                                 cagcTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTc                                                                                    <  1:240990/127‑1 (MQ=255)

                                                                                 cagcTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTc                                                                                    >  2:240990/1‑127 (MQ=255)

                                                                                 cagcTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAg                                                             >  2:849016/1‑150 (MQ=255)

                                                                                 cagcTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAg                                                             >  2:1967444/1‑150 (MQ=255)

                                                                                    cTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGc                                                          <  2:2220391/150‑1 (MQ=255)

                                                                                         ggTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGAttt                                                     <  1:1287538/150‑1 (MQ=255)

                                                                                          gTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTtc                                                    <  1:132821/150‑1 (MQ=255)

                                                                                          gTAGGCCTTGGCGTAGGCGTCGAAGCGCTGCTTCCACCCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTtc                                                    <  1:1807444/150‑1 (MQ=255)

                                                                                             ggCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTtcgtc                                                 <  1:849016/150‑1 (MQ=255)

                                                                                             ggCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTtcgtc                                                 <  1:1967444/150‑1 (MQ=255)

                                                                                               ccTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTcgtcgt                                               >  1:2183597/1‑150 (MQ=255)

                                                                                                cTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTgg                                                                                                                                      <  1:1066834/62‑1 (MQ=255)

                                                                                                cTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTgg                                                                                                                                      >  2:1066834/1‑62 (MQ=255)

                                                                                                cTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGATTCCAGTTCAGGGACTGGCGGGTCAGGGCGATTTCGTCGtt                                              >  1:1156002/1‑150 (MQ=255)

                                                                                                          gCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAg                                                             <  2:132579/125‑1 (MQ=255)

                                                                                                          gCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAg                                                             >  1:132579/1‑125 (MQ=255)

                                                                                                          gCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCggg                                    <  1:1807335/150‑1 (MQ=255)

                                                                                                               gAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCAcc                               >  1:1296267/1‑150 (MQ=255)

                                                                                                                aaGCGCTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAAAGGGCCCAGGTTCCATTTTAGGCCATGGCGGGGCAGGGCGATTTCGTCATTGCCCAGCGCGACACCg                              >  2:533977/1‑150 (MQ=255)

                                                                                                                   cgcTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTgg                           <  1:1521419/150‑1 (MQ=255)

                                                                                                                   cgcTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTgg                           <  1:396498/150‑1 (MQ=255)

                                                                                                                   cgcTGGTTCCACTAGGCTTCAACCGTGGCGATGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTgg                           <  1:494389/150‑1 (MQ=255)

                                                                                                                    gcTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATTTCGGCCGGGATTTCGAACGGGCCGTGCTTCCAGTTCAGGGCCTGGCGTGTCAGGGCGATTTCGTCGTTGCCCACCGGGGCACCGCggg                          >  2:1031243/1‑149 (MQ=255)

                                                                                                                    gcTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGc                          >  2:572316/1‑150 (MQ=255)

                                                                                                                     cTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCggg                                    >  2:731356/1‑139 (MQ=255)

                                                                                                                     cTGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCggg                                    <  1:731356/139‑1 (MQ=255)

                                                                                                                     cTGGTACAACACGGCGTGAAGCTTGGCAACGGCGTCCTTGGCAGCCCTCTCGGCGGAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCCGTTAAGGGCGTGGCGGGTGTTGGCGATTTCGTCGTTGCCCAGCGGGGGACCGTGGCa                         >  2:704981/1‑150 (MQ=255)

                                                                                                                      tGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAggg                                                           >  1:1015504/1‑115 (MQ=255)

                                                                                                                      tGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAggg                                                           <  2:1015504/115‑1 (MQ=255)

                                                                                                                      tGGTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAg                        >  1:1195451/1‑150 (MQ=255)

                                                                                                                        gTTCCACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTc                      <  1:65057/150‑1 (MQ=255)

                                                                                                                            cACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCTTcc                  >  1:200155/1‑150 (MQ=255)

                                                                                                                            cACTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCAGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCTTcc                  <  2:203645/150‑1 (MQ=255)

                                                                                                                            cACTCGGCTTCAACCTTGGCAACGGCGGCCTTCGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAATTCAGGGCCTGGCTGAACATGGCGGTATCGTCGTTGCCAAAAGGGGCTTCGTGGCAgaattcg                  >  1:552097/1‑144 (MQ=255)

                                                                                                                             aCTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCTTCCt                 >  2:1456200/1‑150 (MQ=255)

                                                                                                                             aCTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCTTCCt                 >  1:1503595/1‑150 (MQ=255)

                                                                                                                              cTCGGCTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCTTCCtt                >  2:1913383/1‑150 (MQ=255)

                                                                                                                                   cTTCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCTTCCTTGCCCt           <  2:1488769/150‑1 (MQ=255)

                                                                                                                                    ttCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGTTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCTTCCTTGCCCTg          >  2:768793/1‑150 (MQ=255)

                                                                                                                                    ttCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCt                     >  2:231199/1‑139 (MQ=255)

                                                                                                                                    ttCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCt                     <  1:231199/139‑1 (MQ=255)

                                                                                                                                    ttCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGCTCCAGTTCAGGGCCTGGCgg                                                                  <  1:159871/94‑1 (MQ=255)

                                                                                                                                    ttCAACCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGCTCCAGTTCAGGGCCTGGCgg                                                                  >  2:159871/1‑94 (MQ=255)

                                                                                                                                       aaCCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACggg                                                                                             >  1:1618202/1‑64 (MQ=255)

                                                                                                                                       aaCCTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACggg                                                                                             <  2:1618202/64‑1 (MQ=255)

                                                                                                                                          cTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTc                                                                              <  1:1631381/76‑1 (MQ=255)

                                                                                                                                          cTTGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTc                                                                              >  2:1631381/1‑76 (MQ=255)

                                                                                                                                           ttGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCTTCCTTGCCCTGCTTGTTc   <  2:1195451/150‑1 (MQ=255)

                                                                                                                                            tGGCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCTTCCTTGCCCTGCTTGTTCg  >  1:1223130/1‑150 (MQ=255)

                                                                                                                                              gCAACGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGAtt                                                      >  1:1680049/1‑96 (MQ=255)

CACTTCGTTGATGTAGGCCTGGGCCTTTTCGCTGAAGTCGGCCGGCAGCTCGCCGCTGGCACGGCGCTTGAACTCGGCAGCCAGCTCCGGGTAGGCCTTGGCGTAGGCGTCGAAGCGCTGGTTCCACTCGGCTTCAACCTTGGCACCGGCGGCCTTGGCATCCCACTCGGCGTAGATGTCGGCCGGGATTTCGAACGGGCCGTGGTTCCAGTTCAGGGCCTGGCGGGTCAGGGCGATTTCGTCGTTGCCCAGCGGGGCACCGTGGCAGTCTTCCTTGCCCTGCTTGTTCG  >  NC_002947/5656705‑5656994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`