| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,741,298:1 | +T | 56.3% | intergenic (+489/+263) | gltA → / ← yeiW | citrate synthase/putative oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,741,298 | 1 | . | T | 56.3% | 23.3 / 22.3 | 16 | intergenic (+489/+263) | gltA/yeiW | citrate synthase/putative oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base . (2/5); new base T (6/3); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01 | |||||||||||
GTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCC‑GCCAAC‑TGCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTC > NC_002947/4741155‑4741437 | gTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGaatccag < 2:150645/150‑6 (MQ=255) tgtgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCt < 1:76035/150‑1 (MQ=255) tgtgCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTC‑TGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCt < 1:440039/150‑1 (MQ=255) caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCtt > 2:293112/1‑150 (MQ=255) caCGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CAGCTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCtt > 1:76237/1‑150 (MQ=255) cgTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTCTGCGACT‑TGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCTACCAGC‑CCGCCTCGACCTTGGCCAGCGGCGCCtt < 1:438321/150‑1 (MQ=255) aCCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACGCGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATc > 2:407991/1‑150 (MQ=255) gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc < 1:343129/139‑1 (MQ=255) gTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACcgc > 2:343129/1‑139 (MQ=255) ccAGCTCTGGTGACCTTCGTGCTTGAGCAG‑CTTCT‑GCGACT‑TGCAGTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGTGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGa < 2:74808/150‑1 (MQ=255) cAGCTCTAGTCACCTTCGCACTTGAG‑CA‑GCTCCTGCGACT‑TACATTCGTGAA‑TCAAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGCACTTGTACTTGGAc > 2:22734/1‑150 (MQ=255) gCTCTGGTCACCTTCGTGCTTGAG‑CA‑GCTTC‑TGCGACTTGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGAccc < 2:53535/150‑1 (MQ=255) acttGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcct > 2:315788/4‑150 (MQ=255) ttGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTg > 2:470337/2‑102 (MQ=255) ttGCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcg > 1:481916/2‑150 (MQ=255) ttGCATTCGTGAA‑TCCAGGCACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTg < 1:470337/101‑1 (MQ=255) gCATTCGTGAATCCAGGC‑ACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCcgtcctcgtc < 1:493539/150‑1 (MQ=255) | GTCAGGCTGCCATCCGCCTCCTTGATGGTCGTAATGTGCAGCACGGAATCACGCGGCGTCACCGGCCATGGCGTATTGAACTGGGTATAGGTCCAGCTCTGGTCACCTTCGTGCTTGAG‑AA‑CCTCC‑GCCAAC‑TGCATTCGTGAA‑CCAAGCAACAGGCCCCGGACACGTCCT‑CCTGCAGCGCCTCGACCTTGGCCAGCGGCGCCTTGATCACGGTCACACCGCGATAGGCCTTGTACTTGGACCCGGCCACCTCACTGAGGGACACCTTGATCCCGTCCTCGTC > NC_002947/4741155‑4741437 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |