Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,042,052 T→A 15.5% D32E (GAT→GAA)  PP_3563 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,042,0520TA15.5% 66.3 / 3.1 25D32E (GAT→GAA) PP_3563hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (10/11);  new base A (2/2);  total (12/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTGTAGGATGAGCAGCGCACACCACGACGCTGCCAGTCATAGGGAGATCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGACACACGCCTTGCGCTGTCGCCAGAGGGCACCCGCGACCTGCAACAGGCCATGACCCGCGCCGCCTACC  >  NC_002947/4041904‑4042197
                                                                                                                                                    |                                                                                                                                                 
tgtgTAGGATGAGCAGCGCACACCACGACGCTGCCAGTCATAGGGAGAGCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGCCAGCCACCGGCGAAGAGGCGCCGTTGAccgatt                                                                                                                                                  >  2:234512/1‑150 (MQ=255)
    tAGGATGAGCAGCGCACACCACGACGCTGCCAGTCATAGGGAGATCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGAtcgattcgat                                                                                                                                              >  1:479172/1‑150 (MQ=255)
       gATGAGCAGCGCACACCACGACGCTGCCAGTCATAGGGAGATCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCaa                                                                                                                                           >  2:164302/1‑150 (MQ=255)
       gATGAGCAGCGCACACCACGACGCTGCCAGTCATAGGGAGATCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCaa                                                                                                                                           >  1:549781/1‑150 (MQ=255)
        aTGAGTAGCGCACCCCGCGACGCTGCCAGTCATAGGGAGATCCACATGTGGGTCCTGACAATCGTTGTCGCCTTATCCCTGGGCCCGGTCGTTTCGTCCGGTCCGCTCTCAGCCACCGCCGTAGTGGCGTCGTTGATCTATTCGGTCAAt                                                                                                                                          <  2:383968/150‑1 (MQ=255)
           agcagcGCACACCACGACGCTGCCAGTCATAGGGAGATCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGAATCGATCaactgc                                                                                                                                       <  2:365023/150‑5 (MQ=255)
              agcGCACACCACGACGCTGCCAGTCATAGGGAGATCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTAc                                                                                                                                    <  2:479172/150‑1 (MQ=255)
                       cacGACGCTGCCAGTCATAGGGAGATCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTAc                                                                                                                                    <  2:559850/141‑1 (MQ=255)
                       cacGACGCTGCCAGTCATAGGGAGATCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTAc                                                                                                                                    >  1:559850/1‑141 (MQ=255)
                               tGCCAGTCATAGGGAGATCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAAt                                                                                                                                          <  2:83987/127‑1 (MQ=255)
                               tGCCAGTCATAGGGAGATCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAAt                                                                                                                                          >  1:83987/1‑127 (MQ=255)
                                            gagaTCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAg                                                                                                      >  1:162055/1‑150 (MQ=255)
                                                   acaTGCGCGTCCTGAGATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGATGCACGCAGCCACCGGCGAAGGGGCGCAGTTGAGCGAATCGATAAATGTCTGCCGCAGAAAGGCCCAGACCTGCGGTGGCGAGGcgtcgt                                                                                               >  2:50037/1‑150 (MQ=255)
                                                                     ccGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCa                                                                             >  1:43278/1‑150 (MQ=255)
                                                                             gCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGac                                                                     <  2:362206/150‑1 (MQ=255)
                                                                                ttGTCACTGGGCCTGGTGGTTTCGACCGGGGCGCTGGCAGCCACCTGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGacaca                                                                  <  1:32082/150‑1 (MQ=255)
                                                                                   tCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGACACACGc                                                               >  2:54179/1‑150 (MQ=255)
                                                                                     aCTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGACACACGCCt                                                             >  1:365631/1‑150 (MQ=255)
                                                                                        gggCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGACACACGCCTTgc                                                          <  1:54179/150‑1 (MQ=255)
                                                                                                   tttCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGCTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGACACACGCCTTGCGCTGTCGCCag                                               <  2:190767/150‑1 (MQ=255)
                                                                                                            gggCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGACACACGCCTTGCGCTGTCGCCAGAGGGCAccc                                      >  1:309065/1‑150 (MQ=255)
                                                                                                                     cAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGACACACGCCTTGCGCTGTCGCCAGAGGGCACCCGCGACCTGc                             <  2:309065/150‑1 (MQ=255)
                                                                                                                          aCCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGa                                                                                                       <  2:264036/71‑1 (MQ=255)
                                                                                                                          aCCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGa                                                                                                       >  1:264036/1‑71 (MQ=255)
                                                                                                                               cGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGACACACGCCTTGCGCTGTCGCCAGAGGGCACCCGCGACCTGCAACAGGCCAt                   <  2:365631/150‑1 (MQ=255)
                                                                                                                                           attGATCGAATCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGACACACGCCTTgc                                                          >  2:106250/2‑99 (MQ=255)
                                                                                                                                           attGATCGAATCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGACACACGCCTTgc                                                          <  1:106250/98‑1 (MQ=255)
                                                                                                                                                tcgattcgatCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGACACACGCCTTGCGCTGTCGCCAGAGGGCACCCGCGACCTGCAACAGGCCATGACCCGCGCCGCCTAcc  <  2:43278/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TGTGTAGGATGAGCAGCGCACACCACGACGCTGCCAGTCATAGGGAGATCCACATGCGCGTCCTGACATCCGTTGTCGCCTTGTCACTGGGCCTGGTCGTTTCGACCGGGGCGCTGGCAGCCACCGGCGAAGAGGCGCAGTTGATCGATTCGATCAATGTCTACCGCAGCAAGGCCCAGCCCTGCGGTGGCGAGGCGTCGTTGGAACTGCCGCCGCTCAACAGCGACACACGCCTTGCGCTGTCGCCAGAGGGCACCCGCGACCTGCAACAGGCCATGACCCGCGCCGCCTACC  >  NC_002947/4041904‑4042197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑