Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,459,196	C→T	15.4%	A59A (GCC→GCT)	trkH‑I →	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,459,196	0	C	T	15.4%	50.8 / 2.7	26	A59A (GCC→GCT)	trkH‑I	potassium transporter
Reads supporting (aligned to +/- strand):  ref base C (11/11);  new base T (2/2);  total (13/13)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

GTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGGTGTTCATTCGACACATCAGTTACACGGACGCGTTCTTCG  >  NC_002947/4459076‑4459335                                                                                                                         |                                                                                                                                            gTTGGCCGACAGCATGGTGATACCAATGCTGACGCTGCTGCTGTACGACCGCAGCGACGACCTCGGTGCATTCGTATGGTCGAGCCTGATCGTTCTGTGTCTCGGCCTGCTAATGCTGTTCCGCGGCGTACCCAACACACCGCAACTGCg                                                                                                                >  2:189302/1‑150 (MQ=255)      ccGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCa                                                                                                           <  2:201383/150‑1 (MQ=255)        gTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAgg                                                                                                         <  1:417836/150‑1 (MQ=255)          cAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAggca                                                                                                       >  2:213427/1‑148 (MQ=255)                       ccAGTGTTGACGCGGCTGGTGTTCGACAGCAGCGACGACCTCGGTGCATTCGTGTGGTCGAGCCTGATCGTTCTGTGGGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTc                                                                                          <  2:232827/150‑1 (MQ=255)                         aaTGCTGACGCTGCTGGTGTACGCCCGCAGGGACGACCGCGGTGCATTCGGCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCTCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCac                                                                                        >  2:347425/1‑150 (MQ=255)                         aaTGCTGACGCTGCTGGTGTACGACCGCAGCGACGACACAGGTGCATTCGTCTGGTCGAGCCTGATTGTTCTGTGGGTCGGCCTGCTCATGGTGGCTCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCac                                                                                        >  2:347317/1‑150 (MQ=255)                              tGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTGCACGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACgg                                                                                   >  2:158634/1‑150 (MQ=255)                                aCGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTCTCTACTCACTACGGCa                                                                                 >  2:129780/1‑150 (MQ=255)                                               gACCGCAGCGACGACCTCGGTGCATTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACCGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCgtg                                                                  >  1:494961/1‑150 (MQ=255)                                                aCCGCAGCGACGACCTCGGTGCATTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGacacac                                                                                                                          >  1:15812/1‑93 (MQ=255)                                                aCCGCAGCGACGACCTCGGTGCATTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGacacac                                                                                                                          <  2:15812/93‑1 (MQ=255)                                                   gCAGCGACGACCTCGGTGCATTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCt                                                                             >  2:305292/1‑135 (MQ=255)                                                   gCAGCGACGACCTCGGTGCATTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCt                                                                             <  1:305292/135‑1 (MQ=255)                                                                      aTTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAAt                                           >  1:134935/1‑150 (MQ=255)                                                                         cGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCTCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGgt                                        <  1:347425/150‑1 (MQ=255)                                                                         cGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCTCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGgt                                        <  1:347317/150‑1 (MQ=255)                                                                         cGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCCGGCCAGGGACATGTATCTGCTCACCACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGgt                                        <  1:213427/150‑1 (MQ=255)                                                                               gTCGAGCCTGATCGTTCTGTGTGTCGTCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGGTGTTCAt                                  >  1:72036/1‑150 (MQ=255)                                                                                 cGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGGTGTTCATTc                                <  1:199300/150‑1 (MQ=255)                                                                                    gCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGGTGTTCATTCGac                             <  1:189302/150‑1 (MQ=255)                                                                                             ttCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGGTGTTCATTCGACACATCAGtt                    <  2:494961/150‑1 (MQ=255)                                                                                              tCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGGTGTTCATTCGACACATCAGTTa                   >  2:229243/1‑150 (MQ=255)                                                                                                     gtCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGGTGTTCATTCGACACATCAGTTACACGGAc            <  2:134935/150‑1 (MQ=255)                                                                                                             gCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGGTGTTCATTCGACACATCAGTTACACGGACGCGttctt    <  1:164260/150‑1 (MQ=255)                                                                                                               tCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGGTGTTCATTCGACACATCAGTTACACGGACGCGTTCTTCg  >  2:384756/1‑150 (MQ=255)                                                                                                                         |                                                                                                                                            GTTGGCCGTCAGCATGGTGATTCCAATGCTGACGCTGCTGGTGTACGACCGCAGCGACGACCTCGGTGCATTCGTCTGGTCGAGCCTGATCGTTCTGTGTGTCGGCCTGCTCATGGTGGCCCGCGGCGTACCCGACACACCGCAACTGCGGACCAGGGACATGTATCTGCTCACGACGGCAAGCTGGGTCATCGTGTGCGCATTCGCGGCGCTGCCAATGGTGTTCATTCGACACATCAGTTACACGGACGCGTTCTTCG  >  NC_002947/4459076‑4459335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑