| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,184,168 | A→G | 10.3% | D86G (GAC→GGC) | PP_4564 → | lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,184,168 | 0 | A | G | 10.3% | 96.1 / 3.7 | 40 | D86G (GAC→GGC) | PP_4564 | lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (17/18); new base G (2/2); total (19/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.17e-01 | |||||||||||
CCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCTT > NC_002947/5184020‑5184315 | ccGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCcgac > 2:46091/1‑150 (MQ=255) gatGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAggg > 2:375996/3‑149 (MQ=255) cTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGcc > 2:436777/1‑150 (MQ=255) cTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGcc > 1:411366/1‑150 (MQ=255) gACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTg > 2:531271/1‑150 (MQ=255) cATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTgca < 1:64057/150‑1 (MQ=255) cgcgAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGAGATGGTCGCCGCGGCCCAGGTCACCGACCAGGCCCTGCAGCAAAGc < 1:398598/150‑1 (MQ=255) aGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAg > 1:221556/1‑150 (MQ=255) gggCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAgg > 1:334614/1‑150 (MQ=255) ctgGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAg > 1:94941/1‑150 (MQ=255) ggATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTGCCTCGACTGGCTCGACCGGCTACGTGGCATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGATGCCCGTgttgc < 2:562884/150‑5 (MQ=255) ttGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGa > 2:192653/1‑150 (MQ=255) gcgcGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGAtt < 2:94941/150‑1 (MQ=255) gCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGa < 2:197934/150‑1 (MQ=255) gCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGa < 1:476589/150‑1 (MQ=255) gCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGa > 1:564934/1‑150 (MQ=255) ctggcATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCAc > 1:173818/1‑150 (MQ=255) ttGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGTCa < 1:243393/74‑1 (MQ=25) ttGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCATGTCa > 2:243393/1‑74 (MQ=25) cAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa < 1:119690/92‑1 (MQ=255) cAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCaaa > 2:119690/1‑92 (MQ=255) gACACAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAAATGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCAc < 1:378239/150‑1 (MQ=255) aCTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCAcc > 2:310310/1‑150 (MQ=255) tCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACCTGGTCGTCGGCGACCat < 1:50362/61‑2 (MQ=37) tCAACTGCCCGGCTACCTCGACTGGCGCGACCGGCTACGTGACCTGGTCGTCGGCGACCat > 2:50362/1‑60 (MQ=25) aaCTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAg < 2:459177/59‑1 (MQ=255) aaCTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAg > 1:459177/1‑59 (MQ=255) aaCTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAAc > 2:49668/1‑150 (MQ=255) ggCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTgcgc < 1:531271/150‑1 (MQ=255) gCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGcgcg < 1:436777/150‑1 (MQ=255) gCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGAGCATGAGGCAATTGGTCGATGGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGcgcg > 1:293457/1‑150 (MQ=255) ccTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGAGCGGCAt < 2:564934/150‑1 (MQ=255) tCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGa < 2:173818/150‑1 (MQ=255) tCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCGCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAAcc < 2:89570/150‑1 (MQ=255) cGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCa < 1:310310/150‑1 (MQ=255) gACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAg > 1:248229/1‑150 (MQ=255) gACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATg < 2:334614/150‑1 (MQ=255) gACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATg < 1:46091/150‑1 (MQ=255) cATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCg > 1:243470/1‑150 (MQ=255) aTGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGt < 2:411366/150‑1 (MQ=255) tCGCCAACGACCACGAAACAGACCGAGCCCTGGAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAgg < 2:272367/150‑1 (MQ=255) cgacgaCCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCCGATGCGTGaagacgt > 2:175432/1‑144 (MQ=255) | CCGTGGTCGCTGAGCGACTACCTGGACATGAACCGCGAGCAAAGGGCGCTGCTGGATGATCGGTTGCGCGAGCACCTGGCCTGGCATTGCAAGACTCAACTGCCCGGCTACCTCGACTGGCTCGACCGGCTACGTGACATGGTCGCCGACGACCAGGTCACCGACCAGGCCCTGCAGCAAAGCACCCGCGAGGTCCGTGAGGCAATTGGTCGATTGGCCGAGGAAATCACCCCTTCGGCCACCGAACTGCTGCGCGGCATGAGCGACAACCAGGTAGCGCAGATGCGTGAGGCCTT > NC_002947/5184020‑5184315 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |