Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,385,289 C→T 15.4% intergenic (+34/‑29) lldP → / → lldD L‑lactate permease/L‑lactate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,385,2890CT15.4% 55.2 / 3.4 26intergenic (+34/‑29)lldP/lldDL‑lactate permease/L‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (14/8);  new base T (2/2);  total (16/10)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

TGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGCACACCCTGCGCCACAACGTCTC  >  NC_002947/5385152‑5385436
                                                                                                                                         |                                                                                                                                                   
tGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGc                                                                                                                                         >  2:71434/1‑150 (MQ=255)
  ttCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCaa                                                                                                                                       >  2:277104/1‑150 (MQ=255)
  ttCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCAGGTACTCCCACAAGCaa                                                                                                                                       >  1:510734/1‑150 (MQ=255)
             cGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACaa                                                                                                                                           <  2:188982/135‑1 (MQ=255)
             cGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACaa                                                                                                                                           >  1:188982/1‑135 (MQ=255)
                    cacaGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCAGGTACTCCCACAAGCAACGCTGCGAGAATCCATGa                                                                                                                     <  2:510734/150‑1 (MQ=255)
                     acaGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGAt                                                                                                                    >  1:352565/1‑150 (MQ=255)
                        gCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCAt                                                                                                                       <  1:189633/144‑1 (MQ=255)
                        gCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCAt                                                                                                                       >  2:189633/1‑144 (MQ=255)
                        gCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCAt                                                                                                                 >  2:147634/1‑150 (MQ=255)
                               cttcGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGcc                                                                                                          <  1:27066/150‑1 (MQ=255)
                                      accaTCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCg                                                                                                   >  1:241447/1‑150 (MQ=255)
                                          tcgtcgGCCTGATCACCCTGGTCCATGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTa                                                                                               <  1:277104/150‑1 (MQ=255)
                                                    gATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCca                                                                                                                                              >  1:282683/1‑93 (MQ=255)
                                                    gATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCca                                                                                                                                              <  2:282683/93‑1 (MQ=255)
                                                     aTCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCgg                                                                                    <  2:269539/150‑1 (MQ=255)
                                                        aCCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGccc                                                                                 >  2:106002/1‑150 (MQ=255)
                                                           cTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATcc                                                                                                                         >  2:241549/1‑107 (MQ=255)
                                                           cTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATcc                                                                                                                         <  1:241549/107‑1 (MQ=255)
                                                              gTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGTACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCa                                                                           >  1:316593/1‑150 (MQ=255)
                                                                                                              aGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGCTCATTTCTGCCTATAACGTCTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCg                           >  1:68508/1‑150 (MQ=255)
                                                                                                               ggggCCGGGCGCCGTCGCGCGCCCGGTACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGa                          <  2:316593/150‑1 (MQ=255)
                                                                                                                    cGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTTCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGcaca                     >  2:534003/1‑150 (MQ=255)
                                                                                                                    cGGGCGACGTCGCGCGCCCGGCACTCCCCCAATCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGcaca                     >  2:165287/1‑150 (MQ=255)
                                                                                                                           cGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGCACACCCTgcc              >  2:78406/1‑149 (MQ=255)
                                                                                                                                       ggCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGGCTCTAGTGTCTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCCCTACGCTGCCGGCGGCGGCTAAGCCGAGCACACCCTGCGTCACAACGtctc  <  1:78406/150‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                                   
TGTTCCGCTTCACCGTCAAGCACAGCCTGTTCTTCGCCACCATCGTCGGCCTGATCACCCTGGTCCAGGCTTACTGGCTGACCGGCATGCTGGTTCATCACTAAAGTGAAAGGGGCCGGGCGCCGTCGCGCGCCCGGCACTCCCACAAGCAACGCTGCGAGAATCCATGATCATTTCTGCCTCTACCGACTATCGCGCCGCGGCCCAACGCAAGCTGCCCCCCTTCCTGTTCCACTACGCTGACGGCGGCGCCTACGCCGAGCACACCCTGCGCCACAACGTCTC  >  NC_002947/5385152‑5385436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑