| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,782,792 | G→A | 10.3% | S404N (AGC→AAC) | kefA → | intermediate mechanosensitive channel protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,782,792 | 0 | G | A | 10.3% | 100.5 / 3.7 | 39 | S404N (AGC→AAC) | kefA | intermediate mechanosensitive channel protein |
| Reads supporting (aligned to +/- strand): ref base G (22/13); new base A (2/2); total (24/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.92e-01 | |||||||||||
CCGCCTGTACCAGTTCGAAGTCAACCAGCAACGCGAGCAGATGAGCAGCCCGGTCACCTACGTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTGCGCACCACCCTCGACGAGCAAATGTTCTGGATCCCCAGCAA > NC_002947/5782646‑5782936 | ccGCCTGTACCAGTTCGAAGTCAACCAGCAACGCGAGCAGATGAGCAGCCCGGTCACCTACGTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGa > 1:7833/1‑150 (MQ=255)ccGCCTGTACCAGTTCGAAGTCAACCAGCAACGCGAGCAGATGAGCAGCCCGGTCACCTACGTCGACAAGCTGCTGGAGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGa > 1:411601/1‑150 (MQ=255) ttCGAAGTCAACCAGCAACGCGAGCAGATGAGCAGCCCGGTCACCTACGTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCgg > 2:299162/1‑150 (MQ=255) ttCGAAGTCAACCAGCAACGCGAGCAGATGAGCAGCCCGGTCACCTACGTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCgg < 2:470671/150‑1 (MQ=255) tCGAAGTCAACCAGCAACGCGAGCAGATGAGCAGCCCGGTCACCTACGTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGCATGGGCCATCACCCGCAGCGACCTGTTGGAGCGGc < 1:275002/150‑1 (MQ=255) tCGAAGTCAACCAGCAACGCGAGCAGATGAGCAGCCCGGTCACCTACGTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGc < 2:323915/150‑1 (MQ=255) aGCAGATGAGCAGCCCGGTCACCTACGTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGgcgc > 2:225318/1‑150 (MQ=255) aGCAGATGAGCAGCCCGGTCACCTACGTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGgcgc < 1:248001/150‑1 (MQ=255) cTACGTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCt > 2:295270/1‑96 (MQ=255) cTACGTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCt < 1:295270/96‑1 (MQ=255) cTACGTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCtt > 1:30866/1‑150 (MQ=255) gTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACGGCAGCTGCGCAAGGCCCTGCTGGAACTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCCACGAATCGATCACCTTGCAg > 2:37917/1‑150 (MQ=255) aaGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCacc < 2:7833/150‑3 (MQ=255) gcgcCACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAgctg > 2:259350/1‑150 (MQ=255) aaCCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCTATCACCTTGCAGCTCAACCAGAAGCAGCTGCTgg < 1:225318/150‑1 (MQ=255) aGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAACGACCTGt < 1:2723/66‑1 (MQ=255) aGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAACGACCTGt < 1:2659/66‑1 (MQ=255) aGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAACGACCTGt > 2:2723/1‑66 (MQ=255) aGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAACGACCTGt > 2:2659/1‑66 (MQ=255) gAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACcgcg > 2:491883/1‑150 (MQ=255) aTGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCaga > 1:27014/1‑150 (MQ=255) acacCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTg < 1:142741/150‑1 (MQ=255) cacCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTgc < 2:411601/150‑1 (MQ=255) aaGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACGTGTTGGAGCGGCTGAACCGCGAGCGGACGGCGCTGCTCAACGAATCGAACACCTTGCAGCTCAACCAGGAGCAGCTGCTGGGCACCGCGCAGAGCCTGCGCGCCAGCCTcgac > 2:182717/1‑150 (MQ=255) aaGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGACCTGCTGAACCGCGAGCTGTCCGCGCTGCTCAACGAATCGATCACCTTGTAGTTCACCCACAAGCAGCTGCTGGGCCCCGCGCCGAGCCTGCGCACCACCCTcgac < 2:10198/150‑1 (MQ=255) cccGGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTAGGCACCGCGCAGAGCCTGCGCACCACCCTCGACGAGc > 1:399917/1‑150 (MQ=255) ccTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTc < 2:517940/70‑1 (MQ=255) ccTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTc > 1:517940/1‑70 (MQ=255) gAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTGCGCACCACCCTCGACGAGCAAATGTTc > 2:540143/1‑150 (MQ=255) aGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAAt < 1:18221/68‑1 (MQ=255) aGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAAt > 2:18221/1‑68 (MQ=255) aGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTGCGCACCACCCTCGACGAGCAAATGTTCTg < 2:30866/150‑1 (MQ=255) ggCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTGCGCACCACCCTCGACGAGCAAATGTTCTGGAt > 2:537063/1‑150 (MQ=255) gCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTGCGCACCACCCTCGACGAGCAAATGTTCTGGATc > 2:338684/1‑150 (MQ=255) gCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTGCGCACCACCCTCGACGAGCAAATGTTCTGGATc > 2:81731/1‑150 (MQ=255) cATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTGCGCACCACCCTCGACGAGCAAATGTTCTGGATccc > 1:5974/1‑150 (MQ=255) ccGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTGCGCACCACCCTCGACGAGCAAATGTTCTGGATCCCCAGcaa > 2:401195/1‑150 (MQ=255) ccGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTGCGCACCACCCTCGACGAGCAAATGTTCTGGATCCCCAGcaa > 2:564596/1‑150 (MQ=255) ccGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTGCGCACCACCCTCGACGAGCAAATGTTCTGGATCCCCAGcaa > 1:192362/1‑150 (MQ=255) | CCGCCTGTACCAGTTCGAAGTCAACCAGCAACGCGAGCAGATGAGCAGCCCGGTCACCTACGTCGACAAGCTGCTGGCGGCGCAACCGCAGGAAGATGTGACACCGCAGCTGCGCAAGGCCCTGCTGGAAGTGGCCATCACCCGCAGCGACCTGTTGGAGCGGCTGAACCGCGAGCTGTCGGCGCTGCTCAACGAATCGATCACCTTGCAGCTCAACCAGAAGCAGCTGCTGGGCACCGCGCAGAGCCTGCGCACCACCCTCGACGAGCAAATGTTCTGGATCCCCAGCAA > NC_002947/5782646‑5782936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |