| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,821,953 | T→A | 12.5% | L173Q (CTG→CAG) | metW → | methionine biosynthesis protein MetW |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,821,953 | 0 | T | A | 12.5% | 108.3 / 7.9 | 40 | L173Q (CTG→CAG) | metW | methionine biosynthesis protein MetW |
| Reads supporting (aligned to +/- strand): ref base T (14/21); new base A (2/3); total (16/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
CCGCTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGC > NC_002947/5821807‑5822093 | ccGCTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGc < 2:550508/150‑1 (MQ=255) tGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTc < 1:301967/150‑1 (MQ=255) gTACCTGGTGACCAAAGGCCGCATGCCGGTATCTGACTTCATGCCGTATGCCTGTTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCAGGCGGTCGa < 2:15496/150‑1 (MQ=255) tACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGAc < 1:239313/150‑1 (MQ=255) cgggCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACACCCTGCCAAGGTACTCGACCGCCTGGCGGTCGACCAc < 1:328367/148‑1 (MQ=255) cTGGCGACCAAAGGCCGTATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTCCACCTTCCCCGACTTCGAATAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCAGGCGGTCGagtac < 2:57295/150‑5 (MQ=255) tGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACt > 1:470971/1‑150 (MQ=255) ggCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACtt < 2:386247/150‑1 (MQ=255) ggCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACtt > 1:519949/1‑150 (MQ=255) aCCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCAc < 2:519949/150‑1 (MQ=255) aCCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCAc < 2:394889/150‑1 (MQ=255) aCCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCAc < 2:270033/150‑1 (MQ=255) ccAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCAcc > 1:155298/1‑150 (MQ=255) aTGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACgggtgg < 2:387039/150‑1 (MQ=255) gCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACgggtgggg < 2:356173/150‑1 (MQ=255) ttCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCAGGCGGTCGa > 2:74060/1‑113 (MQ=255) ttCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCAGGCGGTCGa < 1:74060/113‑1 (MQ=255) aTGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCt > 2:412129/1‑150 (MQ=255) gTACAACACGCCGAACATCCACTTCTGCACCTTCGTCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTga < 1:470797/150‑1 (MQ=255) gCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCAt < 2:365166/150‑1 (MQ=255) cACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCACAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTc > 2:262000/1‑150 (MQ=255) aCTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGCACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTg > 1:219096/1‑83 (MQ=255) aCTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTg < 2:219096/83‑1 (MQ=255) aCTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTa > 2:161429/1‑117 (MQ=255) aCTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTa < 1:161429/117‑1 (MQ=255) gCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATg > 1:40427/1‑98 (MQ=255) gCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATg < 2:40427/98‑1 (MQ=255) gaagaaCTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCAt > 2:519304/1‑150 (MQ=255) aagaaCTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATc > 2:481603/1‑150 (MQ=255) gtgCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTcgcg < 1:412129/150‑1 (MQ=255) aCGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCt > 1:282707/1‑150 (MQ=255) gAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGc < 1:134154/123‑1 (MQ=255) gAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGc > 2:134154/1‑123 (MQ=255) gAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGa > 1:350404/1‑150 (MQ=255) aaCGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGAc < 2:496158/150‑1 (MQ=255) tACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAAt < 2:282707/150‑1 (MQ=255) tACTCGACCGCCTGGCGGTCGACCACTTGCACCACAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAAt < 1:262000/150‑1 (MQ=255) tCGACCGCCAGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGGATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGc > 2:556499/1‑150 (MQ=255) gCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCAt < 1:255083/110‑1 (MQ=255) gCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCAt > 2:255083/1‑110 (MQ=255) | CCGCTGGTACCTGGCGACCAAAGGCCGCATGCCGGTATCGGACTTCATGCCGTATACCTGGTACAACACGCCGAACATCCACTTCTGCACCTTCGCCGACTTCGAAGAACTTGTGCACGAACGCCGGGCCAAGGTACTCGACCGCCTGGCGGTCGACCACTTGCACCGCAACGGGTGGGGTGGCCGGCTATGGCCTAATCTTCTAGGTGAGATCGGCATCTACCGCGTCAGCAGTCCGGGCCTGCAAGAGCATCAGCTCGCGGTCTGACGCCCACCGGAGGAATCGC > NC_002947/5821807‑5822093 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |