| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,054,917 | A→G | 10.8% | Q80R (CAG→CGG) | exbD → | TonB‑gated outer membrane transporter gating inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,054,917 | 0 | A | G | 10.8% | 92.4 / 3.0 | 37 | Q80R (CAG→CGG) | exbD | TonB‑gated outer membrane transporter gating inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (14/19); new base G (2/2); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.57e-01 | |||||||||||
CTGATCATCTTCATGGTCGCCGCTCCCTTGGCTACGGTCGACATCAAGGTCGACCTGCCGGCCTCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGATTACGGCGACCTGATGGAAGTGATGAACAACATGCGCGCGGCCGGCTACC > NC_002947/6054769‑6055063 | ctgATCATCTTCATGGTCGCCGCTCCCTTGGCTACGGTCGACATCAAGGTCGACCTGCCGGCCTCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCTACCAGAAGCTGTATGTCGGCGACGATCAg < 1:250679/150‑1 (MQ=255)ctgATCATCTTCATGGTCGCCGCTCCCTTGGCTACGGCCGACATCAAGGTCGACCTGCCGGCCTCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAg < 2:466664/150‑1 (MQ=255) gcTCCCTTGGCTACGGTCGACATCAAGGTCGACCTGCCGGCCTCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCtt < 1:57407/150‑1 (MQ=255) tGGCTACGGTCGACATCAAGGTCGACCTGCCGGCCTCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGa < 2:400289/150‑1 (MQ=255) cTACGGTCGACATCAAGGTCGACCTGCCGGCCTCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGc < 2:371062/150‑1 (MQ=255) aCATCAAGGTCGACCTGCCGGCCTCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCGGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCa > 1:510795/1‑150 (MQ=255) aCATCAAGGTCGACCTGCCGGCCTCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCGGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCa > 1:510743/1‑150 (MQ=255) gCCTCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGc < 2:74976/109‑1 (MQ=255) gCCTCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGc > 1:74976/1‑109 (MQ=255) ccTCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGCTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGaaa < 2:386551/150‑1 (MQ=255) tCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAacc > 1:174871/1‑150 (MQ=255) aaCCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTAGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATAAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAaccac < 2:102296/150‑1 (MQ=255) cGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCAt > 1:476988/1‑150 (MQ=255) ccAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCt > 1:379836/1‑150 (MQ=255) cgcCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAgg < 2:194431/150‑1 (MQ=255) cAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTg > 1:121817/1‑150 (MQ=255) cGGAGAAACCGGTGCTCGGCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAg < 2:174871/150‑1 (MQ=255) ggAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAgg > 1:508160/1‑150 (MQ=255) ggAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAgg > 2:145427/1‑150 (MQ=255) aCCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGa < 1:321583/150‑1 (MQ=255) ttCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAac > 1:11191/1‑110 (MQ=255) ttCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAac < 2:11191/110‑1 (MQ=255) gTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGATTACGGCGAc > 2:227121/1‑150 (MQ=255) cGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGATTACGGCGACCTGAt < 2:476988/150‑1 (MQ=255) gTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGATTACGGCGACCTGATg < 1:52798/150‑1 (MQ=255) ggCGGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGATTACGGCGACCTGATGGAAGt < 2:128950/150‑1 (MQ=255) cGACCAGAAGCTGTATGTCGGCGACGATCGGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGATTACGGCGACCTGATGGAAGtgat < 2:510743/150‑1 (MQ=255) cGACCAGAAGCTGTATGTCGGCGACGATCGGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGATTACGGCGACCTGATGGAAGtgat < 2:510795/150‑1 (MQ=255) gACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGATTACGGCGACCTGATGGAAGtgatg > 2:235540/1‑150 (MQ=255) cAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAg > 2:138641/1‑75 (MQ=255) cAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAg < 1:138641/75‑1 (MQ=255) cAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTGCCAGGCTGACAAAGGCGTGGATTACGGCGACCTGATGGAAGTGATGaac > 1:177796/1‑150 (MQ=255) cTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGATTACGGCGACCTTATGGAAGTGATGAACCACAta > 2:247266/1‑149 (MQ=255) tGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCa > 2:154808/1‑37 (MQ=255) tGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCa < 1:154808/37‑1 (MQ=255) gaTCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGATTACGGCGACCTGATGGAAGTGATGAACAACATGCGCGCGGCCGGCTAc < 1:235540/150‑1 (MQ=255) aTCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCGCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGATTACGGCGACCTGATGGAAGTGATGAACAACATGCGCGCGGCCGGCTAcc < 1:247266/150‑1 (MQ=255) | CTGATCATCTTCATGGTCGCCGCTCCCTTGGCTACGGTCGACATCAAGGTCGACCTGCCGGCCTCAACCGCCAAACCGGCGCCAAGGCCGGAGAAACCGGTGTTCGTCAGCGTCAAGGCCGACCAGAAGCTGTATGTCGGCGACGATCAGGTTGCTGCACCCGACCAGCTTGGCCCGATGCTCGACGCCAAGACCAAGGGTGACAAGGAAACCACCATCTTTTTCCAGGCTGACAAAGGCGTGGATTACGGCGACCTGATGGAAGTGATGAACAACATGCGCGCGGCCGGCTACC > NC_002947/6054769‑6055063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |