| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 26,844 | A→T | 10.5% | intergenic (+40/+145) | PP_0021 → / ← PP_0022 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 26,844 | 0 | A | T | 10.5% | 98.6 / 5.0 | 38 | intergenic (+40/+145) | PP_0021/PP_0022 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (18/16); new base T (2/2); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01 | |||||||||||
TCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTCCT > NC_002947/26703‑26990 | gcccTGATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTa < 1:210876/149‑1 (MQ=255) ccTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACa < 2:95336/150‑1 (MQ=255) cTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAg > 2:491258/1‑150 (MQ=255) cTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAg > 2:392939/1‑150 (MQ=255) ttATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGt > 1:279912/1‑150 (MQ=255) cGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTgg < 2:493755/150‑1 (MQ=255) ggTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGTAAACCACTGTGG‑TTACAGTGAAAAAAAGTGGt < 2:286252/150‑1 (MQ=255) atcatcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACa > 1:481467/1‑150 (MQ=255) tcatcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACaa > 2:411116/1‑150 (MQ=255) catcCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAAc < 1:392939/150‑1 (MQ=255) tCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTAc > 2:241619/1‑150 (MQ=255) agTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGc < 2:481467/150‑1 (MQ=255) tGGCCCGGGAGGAACTGGGAGATCTGCTGGAGCTGACTCAGGCGTGCAGAGGTCTGTTGTAAACCACTTTTTTTACGTCT‑ACCCACAGTAGTTATTACAAAGAAAAATTTGGGTATAACGTAGCTACGACACGCGCCCTGGCCACTcccc < 1:10351/150‑1 (MQ=255) gCCCGGGAGCAACTGGGCTATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTCTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAg < 1:208318/150‑1 (MQ=255) ccGAGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGc > 2:167197/1‑150 (MQ=255) ctgGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCg > 2:316677/1‑150 (MQ=255) ggAGCGGACTCAGGCCGGGGGAGGCCGGTTGTAAACCACGTTTTTTCCCTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGtt < 1:236240/150‑1 (MQ=38) aGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCa < 1:316677/150‑1 (MQ=255) cTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTAc < 1:299337/95‑1 (MQ=255) cTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTAc > 2:299337/1‑95 (MQ=255) tCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGctc < 2:531988/127‑1 (MQ=255) tCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGctc > 1:531988/1‑127 (MQ=255) gCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTGCCCAGGCAAGCGAGCTCCTCATTGATTAGTTCAGTCGGGCGtgg > 1:233685/1‑148 (MQ=255) gCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCg > 1:233950/1‑150 (MQ=255) aggaggCCTGTTGTAAACGACTTTTTTTAACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAacac < 1:441162/150‑1 (MQ=255) gaggCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAacac > 2:368994/1‑148 (MQ=255) gaggCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAacac < 1:368994/148‑1 (MQ=255) cTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTc < 2:471370/126‑1 (MQ=255) cTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTc > 1:471370/1‑126 (MQ=255) tgttgtAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTGGTTAAGTCGGGCGTCGAACACCTAtgctg > 1:260982/1‑150 (MQ=255) aaaCCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGtt > 1:547258/1‑118 (MQ=255) aaaCCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGtt < 2:547258/118‑1 (MQ=255) ttttCACTGTAAACCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTc < 1:361509/105‑1 (MQ=25) ttttCACTGTAAACCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTc > 2:361509/1‑105 (MQ=25) ttttCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCAc < 2:547803/150‑1 (MQ=255) aCTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGACCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTTAGTCGGGCGTCGAACAGCTAGGCTGCTAGGAGCCTTGGGAcgccgc > 2:233268/1‑150 (MQ=255) taaaCCACTGTGG‑TTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGgtg > 1:512788/3‑150 (MQ=255) ccACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGCTCTGACTGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTcc > 2:298745/1‑150 (MQ=255) cacaGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCTAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTCCt > 1:370070/1‑150 (MQ=255) acaGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTc > 2:534413/1‑63 (MQ=255) acaGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTc < 1:534413/63‑1 (MQ=255) acaGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGc < 1:275528/133‑1 (MQ=255) acaGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGc > 2:275528/1‑133 (MQ=255) | TCCCTTATCCGGATGCCGGTTGATCATCCTTATCGCAGAATGGATAGAGTGAAGATGGCCCGGGAGGAACTGGGCGATCTGCTGGAGCTGACTCAGGCCTGAGGAGGCCTGTTGTAAACCACTTTTTTTCACTGT‑AACCACAGTGGTTTACAGTGAAAAAAAGTGGTTTACAACGTAGCTACGACACGCGCCCTGGCCACTCCCCAGGCCAGCGCGCTCCTCATTGATTCGTTCAGTCGGGCGTCGAACACCTATGCTGCTCGGGGCCTTGCCACGCCGCGGTGTCCT > NC_002947/26703‑26990 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |