| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 417,378 | A→G | 8.7% | Q362R (CAG→CGG) | waaG → | lipopolysaccharide glucosyltransferase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 417,378 | 0 | A | G | 8.7% | 128.5 / 2.9 | 46 | Q362R (CAG→CGG) | waaG | lipopolysaccharide glucosyltransferase I |
| Reads supporting (aligned to +/- strand): ref base A (23/19); new base G (2/2); total (25/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGC > NC_002947/417229‑417520 | cGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCgca > 2:219494/1‑150 (MQ=255) gTGCTGGACGAGCCCTTCTAACAAGAGCAGCTCAACGGCTATCTGCAGCACATGCTTGTCCAGACTCAGGCTCTGGCGATGTGGTCGCTTAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGAc < 1:129385/150‑1 (MQ=255) gCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGt > 2:168652/1‑150 (MQ=255) tGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGa > 2:501760/1‑150 (MQ=255) tGGACGAGCCGGTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGa > 2:141492/1‑150 (MQ=255) aaGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCg < 1:168652/150‑1 (MQ=255) tCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCt > 1:421157/1‑150 (MQ=255) tCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCt > 1:374178/1‑150 (MQ=255) ggCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGatat > 2:482222/1‑150 (MQ=255) aTCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTTATATTGGc < 1:219494/150‑1 (MQ=255) gCAGCGCATGCTCGAAGACCCGCAGGCGCGGGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGaa < 1:151929/150‑1 (MQ=255) cAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAAc < 1:501760/150‑1 (MQ=255) aTGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCa > 2:150886/1‑150 (MQ=255) gCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCaaa < 1:150886/150‑1 (MQ=255) gCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGGCAATCGGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCaaa > 1:457702/1‑150 (MQ=255) cTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAAc > 2:231227/1‑150 (MQ=255) tCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACg > 1:292431/1‑150 (MQ=255) tCGAAGACCCGCAGGCGCGTGCCAGCTGGTAGCGCCCAAACGTGATAAAAGTTGCAAGCTCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACg > 1:10609/1‑150 (MQ=255) ccGCAGGCGCGTGCCGGCTGGTCGCGCAAGGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTggg > 2:431853/1‑150 (MQ=255) ccGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTg < 1:342836/96‑1 (MQ=255) ccGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTg > 2:342836/1‑96 (MQ=255) ccGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCa > 1:412630/1‑138 (MQ=255) ccGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCa < 2:412630/138‑1 (MQ=255) ccGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTggg > 1:559445/1‑150 (MQ=255) cAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGcc > 1:285859/1‑150 (MQ=255) aGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCt > 1:440786/1‑150 (MQ=255) cTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGTGCTGTGCGGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAGCCGCATGAAGCGGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTc < 1:32275/150‑1 (MQ=255) gcAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGt > 2:351341/1‑150 (MQ=255) tGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGat < 2:209230/150‑2 (MQ=255) tGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCt > 2:551646/1‑150 (MQ=255) ggCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTg > 2:528815/1‑150 (MQ=255) aTTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCaa < 1:141492/150‑1 (MQ=255) gCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCg > 2:229757/1‑132 (MQ=255) gCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCg < 1:229757/132‑1 (MQ=255) cTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGa < 1:351341/150‑1 (MQ=255) gATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAgg < 1:159746/150‑1 (MQ=255) cgcCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCaa > 2:295805/1‑89 (MQ=255) cgcCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCaa < 1:295805/89‑1 (MQ=255) cgcCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTAt > 2:79104/1‑150 (MQ=255) gTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTg < 2:292431/150‑1 (MQ=255) cAGCATGCTGCGGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAg < 1:7742/132‑1 (MQ=255) cAGCATGCTGCGGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAg > 2:7742/1‑132 (MQ=255) cAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGaa < 1:431853/150‑1 (MQ=255) aGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAACCCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAg < 1:122224/150‑1 (MQ=255) gCATGCTGCGGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAAc > 2:395234/1‑68 (MQ=255) gCATGCTGCGGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAAc < 1:395234/68‑1 (MQ=255) aTGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGgc < 2:440786/150‑1 (MQ=255) | CGACAGCGGCCTGGTGCTGGACGAGCCGTTCGAACAAGAGCAGCTCAACGGCTATCTGCAGCGCATGCTCGAAGACCCGCAGGCGCGTGCCAGCTGGTCGCGCAATGGCCTGGCATTTGCTGATAGCGCCGATCTGTACAGCATGCCGCAGCATGCTGCCGACGTGATCCTGGGGCAAAAAACCGCATGAAGCTGATATTGGCCGAACCCTTCAAACGCCTGTGGGCCGGGCGTGATGCCTTCGACGCCGTGGAGGCGCTGCAAGGCGAGGTCTATCGCGAGCTGGAAGGGC > NC_002947/417229‑417520 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |