Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,089,810 G→C 10.6% Q222H (CAG→CAC)  fumC‑I → class 2 fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,089,8100GC10.6% 109.0 / 5.4 39Q222H (CAG→CAC) fumC‑Iclass 2 fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base G (19/15);  new base C (2/2);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

ACATCAATACGTGAAGACCGGTCGTACTCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCT  >  NC_002947/1089663‑1089957
                                                                                                                                                   |                                                                                                                                                     
aCATCAATACGTGAAGACCGGTCGTACTCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAggg                                                                                                                                                     <  1:63236/150‑1 (MQ=255)
                   ggTCGTACTCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTAcc                                                                                                                                  <  1:145876/150‑1 (MQ=255)
                           tCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCaa                                                                                                                          >  1:479079/1‑150 (MQ=255)
                           tCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCACGGCGGTTCCGCCCTgggc                                                                                                                                     >  2:539758/1‑138 (MQ=255)
                           tCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCACGGCGGTTCCGCCCTgggc                                                                                                                                     <  1:539758/139‑2 (MQ=255)
                              ccTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGc                                                                                                                       >  2:26660/1‑150 (MQ=255)
                                     gACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCACGGCGGTTCCGCCCTgg                                                                                                                                       <  2:232838/127‑1 (MQ=255)
                                     gACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCACGGCGGTTCCGCCCTgg                                                                                                                                       >  1:232838/1‑127 (MQ=255)
                                      aCGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGc                                                                                                              <  2:561531/150‑1 (MQ=255)
                                           aTGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTc                                                                                                         >  1:477864/1‑150 (MQ=255)
                                                      cATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCa                                                                                                                              <  2:324211/119‑1 (MQ=255)
                                                      cATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCa                                                                                                                              >  1:324211/1‑119 (MQ=255)
                                                       aTGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTc                                                                                                   <  1:435662/144‑1 (MQ=255)
                                                       aTGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTc                                                                                                   >  2:435662/1‑144 (MQ=255)
                                                                          gctgggctgCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCGAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCCCA‑CGCGGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGcc                                                                         >  1:148835/1‑150 (MQ=255)
                                                                          gctgggctgCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCACTC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTgggc                                                                         >  1:148822/1‑148 (MQ=255)
                                                                                  gcgcAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCCATCGCACGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAg                                                                 >  2:221628/1‑150 (MQ=255)
                                                                                  gcgcAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAg                                                                 <  1:142416/150‑1 (MQ=255)
                                                                                       gATCAATGCCGCCAAGTCGCACATCGAAGGTGTGCCGCGGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGa                                                            <  2:497188/150‑1 (MQ=255)
                                                                                         tCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGt                                                          <  2:497564/150‑1 (MQ=255)
                                                                                           aaTGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTc                                                        <  2:479079/150‑1 (MQ=255)
                                                                                             tgctgcCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTTGCCTGACCAAGGTCGAGTTCAc                                                      >  2:415715/1‑150 (MQ=255)
                                                                                                  ccAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCg                                                 <  1:264738/150‑1 (MQ=255)
                                                                                                  ccAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCg                                                 <  2:477864/150‑1 (MQ=255)
                                                                                                   cAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTAGCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTGGCGCGCCAGCTCAGTGGCCTGAGCAAGGTTGAGTTCACGCGCgg                                                >  2:310591/1‑150 (MQ=255)
                                                                                                         gCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTc                                                                                                   <  2:108556/94‑1 (MQ=255)
                                                                                                         gCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTc                                                                                                   >  1:108556/1‑94 (MQ=255)
                                                                                                           acaTCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGTGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAg                                                                                                            >  2:229507/1‑83 (MQ=255)
                                                                                                           acaTCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGTGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAg                                                                                                            <  1:229507/83‑1 (MQ=255)
                                                                                                             aTCGAAGGTGTGCTGCCGCACTTGCCGCCGGTGGACCAAGGCGGAAGCGCCGGGGGTATAGGCATCAATGCCAATCGAGCA‑‑TCCAGGTCACACTGCGCTCGCCATCTCAGTGGCCTGACCAAGGTCGAGTTCGCGGCCGTCGATAACCTg                                      >  1:270329/1‑150 (MQ=255)
                                                                                                              tCGAAGGTGTGCTGACGCACTTGCAAGCGCTGGCCCAGGGCGGAACCGCCGTGGCTACCGGCATCAACGCCCATC‑CGAAGTTCGATATCA‑GCTTGTCTTGCCAGGACAGTTGCGGGTCCAAGGGCGAGTTCACGCCCGTCCAGAACCTGt                                     >  2:124572/1‑150 (MQ=255)
                                                                                                               cGAAGGTGTGCGGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGtt                                    <  2:279115/150‑1 (MQ=255)
                                                                                                                 aaGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCAAC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCg                                  >  1:322726/1‑150 (MQ=255)
                                                                                                                            ccGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGc                       >  1:423684/1‑150 (MQ=255)
                                                                                                                               cACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCg                    >  1:293580/1‑150 (MQ=255)
                                                                                                                                      aGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGaca             >  1:190448/1‑150 (MQ=255)
                                                                                                                                               cccAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTgg    <  1:92763/150‑1 (MQ=255)
                                                                                                                                                ccAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCa                  >  2:120896/1‑135 (MQ=255)
                                                                                                                                                ccAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCa                  <  1:120896/135‑1 (MQ=255)
                                                                                                                                                 cAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGGTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCt  >  2:48079/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ACATCAATACGTGAAGACCGGTCGTACTCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCT  >  NC_002947/1089663‑1089957

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑