Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,089,823 G→C 10.3% V227L (GTG→CTG)  fumC‑I → class 2 fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,089,8230GC10.3% 116.4 / 5.4 39V227L (GTG→CTG) fumC‑Iclass 2 fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base G (20/15);  new base C (2/2);  total (22/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

GGTCGTACTCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTC  >  NC_002947/1089682‑1089972
                                                                                                                                             |                                                                                                                                                       
ggTCGTACTCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTAcc                                                                                                                                                 <  1:145876/150‑1 (MQ=255)
        tCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCaa                                                                                                                                         >  1:479079/1‑150 (MQ=255)
        tCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCACGGCGGTTCCGCCCTgggc                                                                                                                                                    <  1:539758/139‑2 (MQ=255)
        tCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCACGGCGGTTCCGCCCTgggc                                                                                                                                                    >  2:539758/1‑138 (MQ=255)
           ccTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGc                                                                                                                                      >  2:26660/1‑150 (MQ=255)
                  gACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCACGGCGGTTCCGCCCTgg                                                                                                                                                      <  2:232838/127‑1 (MQ=255)
                  gACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCACGGCGGTTCCGCCCTgg                                                                                                                                                      >  1:232838/1‑127 (MQ=255)
                   aCGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGc                                                                                                                             <  2:561531/150‑1 (MQ=255)
                        aTGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTc                                                                                                                        >  1:477864/1‑150 (MQ=255)
                                   cATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCa                                                                                                                                             >  1:324211/1‑119 (MQ=255)
                                   cATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCa                                                                                                                                             <  2:324211/119‑1 (MQ=255)
                                    aTGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTc                                                                                                                  >  2:435662/1‑144 (MQ=255)
                                    aTGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTc                                                                                                                  <  1:435662/144‑1 (MQ=255)
                                                       gctgggctgCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCGAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCCCA‑CGCGGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGcc                                                                                        >  1:148835/1‑150 (MQ=255)
                                                       gctgggctgCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCACTC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTgggc                                                                                        >  1:148822/1‑148 (MQ=255)
                                                               gcgcAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCCATCGCACGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAg                                                                                >  2:221628/1‑150 (MQ=255)
                                                               gcgcAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAg                                                                                <  1:142416/150‑1 (MQ=255)
                                                                    gATCAATGCCGCCAAGTCGCACATCGAAGGTGTGCCGCGGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGa                                                                           <  2:497188/150‑1 (MQ=255)
                                                                      tCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGt                                                                         <  2:497564/150‑1 (MQ=255)
                                                                        aaTGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTc                                                                       <  2:479079/150‑1 (MQ=255)
                                                                          tgctgcCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTTGCCTGACCAAGGTCGAGTTCAc                                                                     >  2:415715/1‑150 (MQ=255)
                                                                               ccAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCg                                                                <  1:264738/150‑1 (MQ=255)
                                                                               ccAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCg                                                                <  2:477864/150‑1 (MQ=255)
                                                                                cAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTAGCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTGGCGCGCCAGCTCAGTGGCCTGAGCAAGGTTGAGTTCACGCGCgg                                                               >  2:310591/1‑150 (MQ=255)
                                                                                      gCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTc                                                                                                                  <  2:108556/94‑1 (MQ=255)
                                                                                      gCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTc                                                                                                                  >  1:108556/1‑94 (MQ=255)
                                                                                        acaTCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGTGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAg                                                                                                                           >  2:229507/1‑83 (MQ=255)
                                                                                        acaTCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGTGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAg                                                                                                                           <  1:229507/83‑1 (MQ=255)
                                                                                          aTCGAAGGTGTGCTGCCGCACTTGCCGCCGGTGGACCAAGGCGGAAGCGCCGGGGGTATAGGCATCAATGCCAATCGAGCA‑‑TCCAGGTCACACTGCGCTCGCCATCTCAGTGGCCTGACCAAGGTCGAGTTCGCGGCCGTCGATAACCTg                                                     >  1:270329/1‑150 (MQ=255)
                                                                                           tCGAAGGTGTGCTGACGCACTTGCAAGCGCTGGCCCAGGGCGGAACCGCCGTGGCTACCGGCATCAACGCCCATC‑CGAAGTTCGATATCA‑GCTTGTCTTGCCAGGACAGTTGCGGGTCCAAGGGCGAGTTCACGCCCGTCCAGAACCTGt                                                    >  2:124572/1‑150 (MQ=255)
                                                                                            cGAAGGTGTGCGGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGtt                                                   <  2:279115/150‑1 (MQ=255)
                                                                                              aaGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCAAC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCg                                                 >  1:322726/1‑150 (MQ=255)
                                                                                                         ccGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGc                                      >  1:423684/1‑150 (MQ=255)
                                                                                                            cACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCg                                   >  1:293580/1‑150 (MQ=255)
                                                                                                                   aGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGaca                            >  1:190448/1‑150 (MQ=255)
                                                                                                                            cccAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTgg                   <  1:92763/150‑1 (MQ=255)
                                                                                                                             ccAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCa                                 >  2:120896/1‑135 (MQ=255)
                                                                                                                             ccAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCa                                 <  1:120896/135‑1 (MQ=255)
                                                                                                                              cAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGGTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCt                 >  2:48079/1‑150 (MQ=255)
                                                                                                                                            cgTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCt                                                      <  2:376305/99‑1 (MQ=255)
                                                                                                                                            cgTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCt                                                      >  1:376305/1‑99 (MQ=255)
                                                                                                                                             gTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTc  >  1:205883/1‑150 (MQ=255)
                                                                                                                                             |                                                                                                                                                       
GGTCGTACTCACCTGATGGACGCGATGCCCGTGCGCATGAGCCAGGTGCTGGATGGCTGGGCTGCGCAGATCAATGCTGCCAAGTCGCACATCGAAGGTGTGCTGCCGCACTTGCAGGCGCTGGCCCAGGGCGGAACCGCCGTGGGTACCGGCATCAACGCCCATC‑CGCAGTTCGCTGTCG‑GTTTCGCTCGCCAGCTCAGTGGCCTGACCAAGGTCGAGTTCACGCCCGGCCAGAACCTGTTCGCACTGATCGGCTCGCAGGACACTGCCGTGGCTTTGTCTGGCCAGCTC  >  NC_002947/1089682‑1089972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑