Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 45,680 C→G 9.3% intergenic (‑156/+47) cadA‑I ← / ← PP_0042 cadmium translocating P‑type ATPase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00294745,6800CG 9.3% 131.2 / 5.3 43intergenic (‑156/+47)cadA‑I/PP_0042cadmium translocating P‑type ATPase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (18/21);  new base G (2/2);  total (20/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

TGGGGTCAAGGCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGAAGGTCATCAACCGTGGATAGACCTGGCAGGTA  >  NC_002947/45538‑45829
                                                                                                                                              |                                                                                                                                                     
tGGGGTCAAGGCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTgg                                                                                                                                                >  1:120379/1‑150 (MQ=255)
tGGGGTCAAGGCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTgg                                                                                                                                                >  2:202346/1‑150 (MQ=255)
tGGGGTCAAGGCTTCCAATTGCCCTCTGCAGTGGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTgg                                                                                                                                                <  1:223321/150‑1 (MQ=255)
  gggTCAAGGCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCa                                                                                                                                              <  1:347397/150‑1 (MQ=255)
      cAAGGCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTa                                                                                                                                          <  1:202596/150‑1 (MQ=255)
          gCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCAGTGCCTGGCa                                                                                                                                              <  1:553390/142‑1 (MQ=255)
          gCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCAGTGCCTGGCa                                                                                                                                              >  2:553390/1‑142 (MQ=255)
             tCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATtc                                                                                                                                   >  1:88532/1‑150 (MQ=255)
              ccAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATtct                                                                                                                                  <  2:495910/150‑1 (MQ=255)
                       ctctGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATtgtt                                                                                                                         <  1:441549/150‑1 (MQ=255)
                            cAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCAAGCCAAGGCGTTAACATTTCGAAGCTATGGGGTATCGTGCGTAATAAACAATGCCAGGCACTGCCTGGCATTTATAAATAATCTATTggttctgtt                                                                                                                    <  2:165116/150‑1 (MQ=255)
                              gTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTAt                                                                                                                                         <  2:467075/127‑1 (MQ=255)
                              gTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTAt                                                                                                                                         >  1:467075/1‑127 (MQ=255)
                                     ggCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGtt                                                                                                           <  2:335257/149‑1 (MQ=255)
                                                    ttggCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACttgttgttg                                                                                            <  1:202346/150‑1 (MQ=255)
                                                    ttggCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACttgttgttg                                                                                            >  1:44640/1‑150 (MQ=255)
                                                     tggCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGa                                                                                           <  1:509463/150‑1 (MQ=255)
                                                         tAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCa                                                                                       >  1:202127/1‑150 (MQ=255)
                                                         tAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCa                                                                                       >  1:157241/1‑150 (MQ=255)
                                                              tCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCg                                                                                  >  2:470948/1‑150 (MQ=255)
                                                               cTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATCTGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGa                                                                                 <  1:61396/150‑1 (MQ=255)
                                                                tATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGaa                                                                                <  2:44640/150‑1 (MQ=255)
                                                                        ggTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGa                                                                        <  2:88532/150‑1 (MQ=255)
                                                                               ccGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATtgtt                                                                                                                         <  2:176450/94‑1 (MQ=255)
                                                                               ccGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATtgtt                                                                                                                         >  1:176450/1‑94 (MQ=255)
                                                                                     ccAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTc                                                           <  2:123867/150‑1 (MQ=255)
                                                                                     ccAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTc                                                           >  2:463330/1‑150 (MQ=255)
                                                                                           gTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGc                                                     <  1:361289/150‑1 (MQ=255)
                                                                                           gTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATACCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGc                                                     >  2:409969/1‑150 (MQ=255)
                                                                                             tAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGt                                                   >  2:503128/1‑150 (MQ=255)
                                                                                                 aTTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTta                                                                                                         <  1:492298/92‑1 (MQ=255)
                                                                                                 aTTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTta                                                                                                         >  2:492298/1‑92 (MQ=255)
                                                                                                      gAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAAtt                                                                                                                            >  1:468229/1‑68 (MQ=255)
                                                                                                      gAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAAtt                                                                                                                            <  2:468229/68‑1 (MQ=255)
                                                                                                      gAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGa                                          >  1:231236/1‑150 (MQ=255)
                                                                                                      gAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCACGTGTTAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTTCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGa                                          >  1:14510/1‑150 (MQ=255)
                                                                                                           tATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTg                                                  >  1:347767/1‑137 (MQ=255)
                                                                                                           tATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTg                                                  <  2:347767/137‑1 (MQ=255)
                                                                                                                               aaaaaaTGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGAAGGTCATCAACCGTGGa                 <  1:463330/150‑1 (MQ=255)
                                                                                                                                    aTGCCAGGCAGTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGAAg                                >  2:157516/1‑130 (MQ=255)
                                                                                                                                    aTGCCAGGCAGTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGAAg                                <  1:157516/130‑1 (MQ=255)
                                                                                                                                        cAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTc                                       <  2:395936/119‑1 (MQ=255)
                                                                                                                                        cAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTc                                       >  1:395936/1‑119 (MQ=255)
                                                                                                                                              cTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGAAGGTCATCAACCGTGGATAGACCTGGCAGGTa  >  1:478957/1‑150 (MQ=255)
                                                                                                                                              |                                                                                                                                                     
TGGGGTCAAGGCTTCCAATTGCCCTCTGCAGTTGGGGAGCCGTCTCTGTTGGTTGGCTAAGATCTATTGCACGGTTTGCCCGAGCCCAGGCGTTAACATTTCGAAGCTATGGGGTATCGGGCGTAATAAAAAATGCCAGGCACTGCCTGGCATTTATGAATTCTCTAATTGTTCTGTTCAAATCGTTTACTACTTGTTGTTGATGCATTGCGAATACATCGAGTAACGAACAGTCTTGAGCGTGCCCTGGGAGTCCTCGAAGGTCATCAACCGTGGATAGACCTGGCAGGTA  >  NC_002947/45538‑45829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑