| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,478,203 | T→G | 15.4% | S284A (TCC→GCC) | PP_1291 → | PhoH family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,478,203 | 0 | T | G | 15.4% | 51.9 / 5.7 | 26 | S284A (TCC→GCC) | PP_1291 | PhoH family protein |
| Reads supporting (aligned to +/- strand): ref base T (9/13); new base G (2/2); total (11/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
ACTGCTGCTACTCGACCTGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGAAG > NC_002947/1478058‑1478335 | aCTGCTGCTACTCGGCCTGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCgg < 1:472844/150‑1 (MQ=255) tgctACTCGACCTGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTaaaa < 1:367977/150‑1 (MQ=255) gCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGc > 2:382480/1‑150 (MQ=255) aGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCa > 2:514081/1‑150 (MQ=255) gAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTTTTCGCCCTGCGGGACCCGGATATCCCCCTAGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATg < 1:37922/150‑1 (MQ=255) ccTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCa < 1:370383/150‑1 (MQ=255) tGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGc < 1:514081/150‑1 (MQ=255) gggCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCaa < 2:521732/150‑1 (MQ=255) cgACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGcat < 2:218678/150‑1 (MQ=255) gcTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCa < 1:382480/150‑1 (MQ=255) cGCCCTGCTGGACCCGGTTATCCACCTGCTGAACCTGACCGGCGCCCCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCt < 1:343674/150‑1 (MQ=255) ctgctgGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGAt > 2:350964/1‑150 (MQ=255) ctgGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCa > 1:333409/1‑97 (MQ=255) ctgGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCa < 2:333409/97‑1 (MQ=255) aCCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGc > 1:338737/1‑150 (MQ=255) gAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATAGCCACCCGCCGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTAc < 2:503652/150‑1 (MQ=255) ccTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAggg > 1:381960/1‑114 (MQ=255) ccTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAggg < 2:381960/114‑1 (MQ=255) ccTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGa > 2:9213/1‑150 (MQ=255) ccTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGa > 2:475565/1‑150 (MQ=255) tttaCCGGAGCCGGCGGCGCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGaa > 1:513521/4‑150 (MQ=255) ttaCCGGAGCCGGCGGCGCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGa > 2:206911/3‑55 (MQ=25) ttaCCGGAGCCGGCGGCGCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGa < 1:206911/53‑1 (MQ=21) ttaCCGGAGCCGGCGGCGCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGAAg < 2:513521/148‑1 (MQ=255) gccgGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGcc < 2:388619/128‑1 (MQ=255) gccgGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGcc > 1:388619/1‑128 (MQ=255) | ACTGCTGCTACTCGACCTGCACCAGGAGCCTTTGCTGCACCAGGAAGCCTGGGGCCTGAAACCACGCGACATTCATCAAAGCCTGGCGCTGTTCGCCCTGCTGGACCCGGATATCCACCTGGTGAACCTGACCGGCGCCGCCGGCTCCGGTAAAACCATCCTGGCACTGGCTGCGGCCATCGAGCAAACCATGGTCAGCAAGCGCTACCGGCGCATCATCGCCACCCGCAGCGTGCAGGGGCTGGATCAGGAGATTGGCTTCCTGCCGGGTACCGAAG > NC_002947/1478058‑1478335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |