Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,647,612 G→A 100% D384D (GAC→GAT)  oprB‑II ← carbohydrate‑selective porin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,647,6120GA100.0% 83.6 / NA 27D384D (GAC→GAT) oprB‑IIcarbohydrate‑selective porin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/14);  total (13/14)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGTCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTGCACATA  >  NC_002947/1647477‑1647755
                                                                                                                                       |                                                                                                                                               
cTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCcggc                                                                                                                                   >  2:457367/1‑150 (MQ=255)
 tGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGNGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCcggcg                                                                                                                                  >  1:359784/1‑150 (MQ=255)
 tGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCcggcg                                                                                                                                  <  1:175431/150‑1 (MQ=255)
   cGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCggcggc                                                                                                                                <  1:31226/150‑1 (MQ=255)
          ccgcccgGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTg                                                                                                                         >  1:553509/1‑150 (MQ=255)
                   tGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAggcgg                                                                                                                <  1:393195/150‑1 (MQ=255)
                    ggCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAggcggg                                                                                                               <  1:222465/150‑1 (MQ=255)
                           gTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGACCAGTGGCCTGGTTGACCAGGCGGGCGTTCtt                                                                                                        <  1:12677/150‑1 (MQ=255)
                            tACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTg                                                                                                       <  2:359784/150‑1 (MQ=255)
                              cTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCg                                                                                                     >  2:269821/1‑150 (MQ=255)
                                 cAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATa                                                                                                  <  2:201651/150‑1 (MQ=255)
                                                      ccAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCTTGGTTGTCGTAATCGTAGAGGCCAGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGATGTGCAcgcg                                                                             >  1:183365/1‑150 (MQ=255)
                                                             gCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAgg                                                                      >  2:76467/1‑150 (MQ=255)
                                                               cAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGc                                                                    <  2:24019/150‑1 (MQ=255)
                                                                       tGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGa                                                            <  2:341625/150‑1 (MQ=255)
                                                                                 cAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCtt                                                  <  2:553509/150‑1 (MQ=255)
                                                                                            tGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCAt                                       >  1:335203/1‑150 (MQ=255)
                                                                                              tACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTg                                                                                        >  2:77593/1‑99 (MQ=255)
                                                                                              tACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTg                                                                                        <  1:77593/99‑1 (MQ=255)
                                                                                                          tGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTg                         >  1:439568/1‑150 (MQ=255)
                                                                                                          tGCACTGGCAGGAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTg                         >  2:493189/1‑150 (MQ=255)
                                                                                                                     gAAGCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTcc              >  1:365840/1‑150 (MQ=255)
                                                                                                                        gCCCGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTcctgc           <  1:444458/150‑1 (MQ=255)
                                                                                                                         cccGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGAt                                                           <  2:151533/101‑1 (MQ=255)
                                                                                                                         cccGGGTTGTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGAt                                                           >  1:151533/1‑101 (MQ=255)
                                                                                                                           cGGGTTGTCGTAATCATAGAGGCGCGAGGCCCGGTTGACCAGGCGGGCGTGCTTGCGATAGGCAGGGTTGACGTGCAGGCGGGCCAGGGCGAAGCCGATGTCGTGCGTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTcctgcctg        >  1:158493/1‑150 (MQ=255)
                                                                                                                                 gTCGTAATCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTGCACATa  <  2:238181/150‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                               
CTGCGACACCCCGCCCGGGTGGCGGATGTACTGCAGGTTGGGGCGTACCGTGAGCCAGTCGGCCAAGTGAATGCCGTAATACAGCTCGGCGCTGTACTCGGTGTCCTGCACTGGCAGGAAGCCCGGGTTGTCGTAGTCATAGAGGCCGGCGGCCTGGTTGACCAGGCGGGCGTTCTTGCGATAGGCAGGGTTGACGTGCACGCGGGCCAGGGCGAAACCGATGTCGTCCTTGGCGCGGGCATCGAAAGGCCCTTTGAATACCAGTCCTGCCTGCACATA  >  NC_002947/1647477‑1647755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑