| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,940,446 | G→T | 18.2% | Q292K (CAG→AAG) | PP_1740 ← | beta (1‑6) glucans synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,940,446 | 0 | G | T | 18.2% | 45.5 / 3.2 | 22 | Q292K (CAG→AAG) | PP_1740 | beta (1‑6) glucans synthase |
| Reads supporting (aligned to +/- strand): ref base G (9/9); new base T (2/2); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.24e-01 | |||||||||||
CCGTCAGCACGGCGCTGGCCAGCAACCACTGCGGCCAGTCATGCAGGTTGCTCACGGGCCCTTCGAGCACGCCTTTGTCCTGTCGGTCCGCGTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGAAGCCACGGATGAAGCGCGCCTCATTGACCCGGCTGGGCACCGCCGTTTCGCGTTGGCGGCCTTCGCTGGGCCAGCCGGTTTCGCCAATCA > NC_002947/1940299‑1940592 | ccGTCAGCACGGCGCTGGCCAGCAACCACTGCGGCCAGTCATGCAGGTTGCTCACGGGCCCTTCGAGCACGCCTTTGTCCTGTCGGTCCGCGTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGAt < 2:183680/150‑1 (MQ=255) ccAGTCATGCAGGTTGCTCACGGGCCCTTCGAGCACGCCTTTGTCCTGTCGGTCCGCGTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCAtt > 1:113913/1‑150 (MQ=255) tGCAGGTTGCTCACGGGCCCTTCGAGCACGCCTTTGTCCTGTCGGTCCGCGTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCg < 1:514507/150‑1 (MQ=255) gggCCCTTCGAGCACGCCTTTGTCCTGTCGGTCCGCGTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGaa < 1:535507/150‑1 (MQ=255) cccTTCGAGCACGCCTTTGTCCTGTCGGTCCGCGTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGAAGcc > 2:57359/1‑150 (MQ=255) cTTTGTCCTGTCGGTCCGCGTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCtt < 2:134989/87‑1 (MQ=255) cTTTGTCCTGTCGGTCCGCGTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCtt > 1:134989/1‑87 (MQ=255) cTGTCGGTCCGCGTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGAAGCCACGGATGAAGCGCGCCTCAtt < 1:220801/150‑1 (MQ=255) tGTCGGTCCGCGTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGAAGCCACGGATGAAGCGCGCCTCATTg > 2:182167/1‑150 (MQ=255) gTCGGTCCGCGTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGAAGCCACGGATGAAGCGCGCCTCATTGa < 1:182167/150‑1 (MQ=255) cgcgTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGAAGCCACGGATGAAGCGCGCCTCATTGACCCGGCt < 2:113913/150‑1 (MQ=255) ccccAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGAAGCCACGGATGAAGCGCGCCTCATTGACCCGGCTGGGCACCGCCGtt > 2:200207/1‑150 (MQ=255) cccAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGAAGCCACGGATGAAgcgcgc > 1:209872/1‑121 (MQ=255) cccAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGAAGCCACGGATGAAgcgcgc < 2:209872/121‑1 (MQ=255) ccTCATTGGCGCGTTTCCAGGGCTTATCGAAGGCTTCGATCAGGTTGt < 1:476506/48‑1 (MQ=255) ccTCATTGGCGCGTTTCCAGGGCTTATCGAAGGCTTCGATCAGGTTGt < 1:196013/48‑1 (MQ=255) ccTCATTGGCGCGTTTCCAGGGCTTATCGAAGGCTTCGATCAGGTTGt > 2:196013/1‑48 (MQ=255) ccTCATTGGCGCGTTTCCAGGGCTTATCGAAGGCTTCGATCAGGTTGt > 2:476506/1‑48 (MQ=255) ttGGCGCGGTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGAAGCCACGGATGAAGCGCGCCTCATTGACCCGGCTGGGCACCGCCGTTTCGCGTTGGCGGCCTTCGCTGGGCCAg > 2:33111/1‑150 (MQ=255) gcgTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGa < 2:139178/71‑1 (MQ=255) gcgTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGa > 1:139178/1‑71 (MQ=255) ccTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGAAGCCACGGATGAAGCGCGCCTCATTGCCCCGGCTGGGCACCGCCGTTTCGCGTTGGCGGCCTTCGCTGGGCCAGCCGGTTTCGCCAATCa > 2:461479/2‑150 (MQ=255) | CCGTCAGCACGGCGCTGGCCAGCAACCACTGCGGCCAGTCATGCAGGTTGCTCACGGGCCCTTCGAGCACGCCTTTGTCCTGTCGGTCCGCGTCGTATAGCCCCCAGTAACCACCCACCGCGCCCTCATTGGCGCGTTTCCAGGGCTGATCGAAGGCTTCGATCAGGTTGTAGTGCCAGCCATTGCGCTCGGCCATGGCCACGAAGCCACGGATGAAGCGCGCCTCATTGACCCGGCTGGGCACCGCCGTTTCGCGTTGGCGGCCTTCGCTGGGCCAGCCGGTTTCGCCAATCA > NC_002947/1940299‑1940592 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |