| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,080,393 | G→C | 23.7% | A340A (GCG→GCC) | earP → | EF‑P arginine 32 rhamnosyl‑transferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,080,393 | 0 | G | C | 23.7% | 23.5 / 7.6 | 17 | A340A (GCG→GCC) | earP | EF‑P arginine 32 rhamnosyl‑transferase |
| Reads supporting (aligned to +/- strand): ref base G (6/7); new base C (2/2); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.05e-01 | |||||||||||
TATGTGCAGGACGAGAATGCCCATTGGGAAAAGCTCGAAGCTTTCCTCGCGCATTATCGCTGCGGCCTGTCAGACGATGCCGATGCAGCCCTGTTGGGCCTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGACAGGCCGCTCAGCCGGACCTTGCCACAGCGCTAGTACACTTTTACCGAAATT > NC_002947/2080244‑2080499 | tATGTGCAGGACGAGAATGCCCATTGGGAAAAGCTCGAAGCTTTCCTCGCGCATTATCGCTGCGGCCTGTCAGACGATGTCGATGCAGCCCTGTTGGGCCTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTggcgggcg < 1:39856/150‑1 (MQ=255) tgtgCAGGACGAGAATGCCCATTGGGAAAAGCTCGAAGCTTTCCTCGCGCATTATCGCTGCGGCCTGTCAGACGATGCCGATGCAGCCCTGTTGGGCCTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGggcggc > 1:55146/1‑150 (MQ=255) gCAGGACGAGAATGCCCATTGGGAAAAGCTCGAAGCTTTCCTCGCGCATTATCGCTGCGGCCTGTCAGACGATGCCGATGCAGCCCTGTTGGGCCTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCg > 2:103349/1‑150 (MQ=255) aTTGGGAAAAGCTCGAAGCTTTCCTCGCGCATTATCGCTGCGGCCTGTCAGACGATGCCGATGCAGCCCTGTTGGGCCTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAAc > 2:426378/1‑150 (MQ=255) gggAAAAGCTCGAAGCTTTCCTCGCGCATTATCGCTGCGGCCTGTCAGACGATGCCGATGCAGCCCTGTTGGGCCTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTgc < 2:283182/150‑1 (MQ=255) tCGAAGCTTTCCTCGCGCATTATCGCTGCGGCCTGTCAGACGATGCCGATGCAGCCCTGTTGGGCCTGTGACGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATg > 1:214343/1‑150 (MQ=255) gcATTATCGCTGCGGCCTGTCAGACGATGCCGATGCAGCCCTGTTGGGCCTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGgc < 1:426378/150‑1 (MQ=255) ccgatgcAGCCCTGTTGGGCCTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGACAGGCCGCTCAGCCGGACCTTGCca < 2:16752/150‑1 (MQ=255) cccTGTTGGGCCTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTgggggg < 1:218713/111‑1 (MQ=255) cccTGTTGGGCCTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTgggggg > 2:218713/1‑111 (MQ=255) ttGGGCCTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGACAGGCCGCTCAGCCGGACCTTGCCACAGCGCTAGTacac < 2:410780/150‑1 (MQ=255) cTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGACAGGCCGCTCAGCCGGACCTTGCCACAGCGCTAGTACACTTTTAc > 1:430776/1‑150 (MQ=255) gTGCCTGGAAAATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGACAGGCCGCTCAGCCGGACCTTGCCACAGCGCTAGTACACTTTTACCGAAAtt < 2:343136/150‑1 (MQ=255) aatgcTGGCGGGCCGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGACAGGCCGCTCAGCCGGACCTTGCCACAgc < 1:330940/93‑1 (MQ=255) aatgcTGGCGGGCCGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGACAGGCCGCTCAGCCGGACCTTGCCACAgc > 2:330940/5‑97 (MQ=255) gCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGACAGGCCGCTCAGCCGGACCTTGCCACAGCGCTAGTACACTTTTACCGaa > 1:53375/1‑115 (MQ=255) gCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGACAGGCCGCTCAGCCGGACCTTGCCACAGCGCTAGTACACTTTTACCGaa < 2:53375/115‑1 (MQ=255) tGGCGGGCCGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGACAGGCCGCTCAGCCGGACCtt < 2:323604/84‑1 (MQ=255) tGGCGGGCCGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGACAGGCCGCTCAGCCGGACCtt > 1:323604/1‑84 (MQ=255) gggcggCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGa > 1:353112/1‑59 (MQ=255) gggcggCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGa < 2:353112/59‑1 (MQ=255) | TATGTGCAGGACGAGAATGCCCATTGGGAAAAGCTCGAAGCTTTCCTCGCGCATTATCGCTGCGGCCTGTCAGACGATGCCGATGCAGCCCTGTTGGGCCTGTGGCGTGCCTGGAACATGGATTTCGACATGGGCCAGGCCTGGCGGGCGGCCCGCCAGCATTGGCCGGAACTGCAGCAGCATGCTCGCCTTTGGGGGGCGCGACAGGCCGCTCAGCCGGACCTTGCCACAGCGCTAGTACACTTTTACCGAAATT > NC_002947/2080244‑2080499 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |