Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,735,035	G→A	26.7%	T630T (ACG→ACA)	PP_2394 →	leucine‑rich repeat‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,735,035	0	G	A	26.7%	18.0 / 5.5	15	T630T (ACG→ACA)	PP_2394	leucine‑rich repeat‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (5/6);  new base A (2/2);  total (7/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.46e-01

ACCGGTCGCTGGCGATTCATGGCCTGGATGATCAGCATCTGCGGGCATGGCATGTGTATGGCCGACCGCCGGAGGCCGCGCTGCTCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCAACTGCTGGAGCGGGTCATGCGCCTTCCACAAGCCGCCGGCAAGGAAGGAGCAGCGTTGGCCGATGTGGCTTGGGCCGGGCGACGAGAACTGCTTCAGGCGTT  >  NC_002947/2734908‑2735181                                                                                                                                |                                                                                                                                                   aCCGGTCGCTGGCGATTCATGGCCTGGATGATCAGCATCTGCGGGCATGGCATGTGTATGGCCGACCGCCGGAGGCCGCGCTGCTCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAgg                                                                                                                              <  1:441098/150‑1 (MQ=255)  ccGGTCGCTGGCGATTCATGGCCTGGATGATCAGCATCTGCGGGCATGGCATGTGTATGGCCGACCGCCGGAGGCCGCGCTGCTCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAggg                                                                                                                             >  2:497450/1‑150 (MQ=255)      tCGCTGGCGATTCATGGCCTGGATGATCAGCATCTGCGGGCATGGCATGTGTATGGCCGACCGCCGGAGGCCGCGCTGCTCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAGGGCGgt                                                                                                                         <  1:227480/150‑1 (MQ=255)                       ccTGGATGATCAGCATCTGCGGGCATGGCATGTGTATGGCCGACCGCCGGAGGCCGCGCTGCTCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCa                                                                                                        >  1:12653/1‑150 (MQ=255)                        cTGGATGATCAGCATCTGCGGGCATGGCATGTGTATGGCCGACCGCCGGAGGCCGCGCTGCTCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACATTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCaa                                                                                                       >  2:231066/1‑150 (MQ=255)                        cTGGATGATCAGCATCTGCGGGCATGGCATGTGTATGGCCGAACGCCGGAGGCCGCGCTGCTCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACATTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCaa                                                                                                       >  2:163495/1‑150 (MQ=255)                                           gggCATGGCATGTGTATGGCCGACCGCCGGAGGCCGCGCTGCTCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCAACTGCTGGAGCGGGTCATgc                                                                                    <  2:12653/150‑1 (MQ=255)                                                 ggcatgTGTATGGCCGACCGCCGGAGGCCGCGCTGCTCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACATTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCAACTGCTGGAGCGGGTCATGCGCCTTc                                                                              <  1:163495/150‑1 (MQ=255)                                                 ggcatgTGTATGGCCGACCGCCGGAGGCCGCGCTGCTCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACATTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCAACTGCTGGAGCGGGTCATGCGCCTTc                                                                              <  1:231066/150‑1 (MQ=255)                                                                       ggAGGCCGCGCTGCTCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAAGGCGGTGTACCCGTAGACCAGTTGCGCCAGGTCCTTGTCATGCGCCTTCCACAAGCCGCCGGCaaggaagg                                                        <  1:154447/150‑1 (MQ=255)                                                                                  tgctCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCAACTGCTGGAGCGGGTCATGCGCCTTCCACAAGCCGCCGGCAAGGAAGGAGCAGCGTTgg                                             >  2:394327/1‑150 (MQ=255)                                                                                              tCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCAACTGCTGGAGCGGGTCCAGCTCGCGGCACAAGCCGCCGGCAAGGAAGGAGCAGCGTTGGCCGATGTGGCtt                                 >  1:317834/1‑150 (MQ=255)                                                                                                          ggCTCGCCGGCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCAACTGCTGGAGCGGGTCATGCGCCTTCCACAAGCCGCCGGCAAGGAAGGAGCAGCGTTGGCCGATGTGGCTTGGGCCGGGcgac                     <  1:394327/150‑1 (MQ=255)                                                                                                                  ggCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCAActgctg                                                                                                 >  1:75107/1‑66 (MQ=255)                                                                                                                  ggCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCAActgctg                                                                                                 <  2:75107/66‑1 (MQ=255)                                                                                                                             tcagTTTGTCAATCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCAACTGCTGGAGCTGGTCATGCGCCTTCCACAAGCCGCCGGCAAGGAAGGAGCAGCGTTGGCCTATGTGGCTTGTGCCGGGCGACGAGAACTGCTTCAGGCGtt  <  2:40428/147‑1 (MQ=255)                                                                                                                                |                                                                                                                                                   ACCGGTCGCTGGCGATTCATGGCCTGGATGATCAGCATCTGCGGGCATGGCATGTGTATGGCCGACCGCCGGAGGCCGCGCTGCTCGACACCGTCACGCGCGTGCGGCTCGCCGGCAGGATTGGTACGTTGGTCAACCAGTTGCGCGAGGGCGGTGTAACCGCAGACCCGCAACTGCTGGAGCGGGTCATGCGCCTTCCACAAGCCGCCGGCAAGGAAGGAGCAGCGTTGGCCGATGTGGCTTGGGCCGGGCGACGAGAACTGCTTCAGGCGTT  >  NC_002947/2734908‑2735181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑