| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 2,994,670 | G→T | 19.1% | P213H (CCC→CAC) | PP_2618 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 2,994,670 | 0 | G | T | 19.1% | 37.6 / 2.1 | 20 | P213H (CCC→CAC) | PP_2618 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (7/9); new base T (2/2); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
GATGTGCGTGTCGTTGACCAACACCGACAGCATGATCTGGCGGCCACGCGGCGGAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACTTGAATTGGCCAGGGCGCAGCAGGTAGTCGTCACTACGGATATAGGTACTGCGCAGCGCCTTGGCCGGGTCCTGCTCCAGTAGCTGGTACACGGTGTTGCGCAGCAGTGAGTCGTCGCGCAGTTGC > NC_002947/2994531‑2994813 | gATGTGCGTGTCGTTGACCAACACCGACAGCATGATCTGGCGGCCACGCGGCGGAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACgg < 1:232869/150‑1 (MQ=255) tgtCGTTGACCAACACCGACAGCATGATCTGGCGGCCACGCGGCGGAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACt > 1:121800/1‑150 (MQ=255) gtCGGTGCCCAACACGAACAGCATGATCTGGCGGCCACGCGGCGGAATGCGCACTACTTCCCGCCAACTGGCGTTTTGATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACtt < 2:127997/150‑1 (MQ=38) ccAACACCGACAGCATGATCTGGCGGCCACGCGGCGGAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACTTGAATTGGc < 1:515706/150‑1 (MQ=255) ccGACAGCATGATCTGGCGGCCACGCGGCGGAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGATTCGGCGTGGATGGGTTGGAACGGGATGGACTTGAATTGGCCAGGgc > 2:307078/1‑150 (MQ=255) aCAGCATGATCTGGCGGCCACGCGGCGGAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACTTGAATTGGCCAGGGCgca > 1:286684/1‑150 (MQ=255) cATGATCTGGCGGCCACGCGGCGGAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATgg > 2:321615/1‑110 (MQ=255) cATGATCTGGCGGCCACGCGGCGGAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATgg < 1:321615/110‑1 (MQ=255) aTGATCTGGCGGCCACGCGGCGGAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGCATGGGTTCGAACGGGATGTAATTTAATTGGCCAGGGAGCAGCAgg > 2:16372/1‑150 (MQ=255) gATCTGGCGGCCACGCGGCGGAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACTTGAATTGGCCAGGGCGCAGCAGGTa < 1:63931/150‑1 (MQ=255) ggcggAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACTTGAATTGGCCAGGGCGCAGCAGGTAGTCGTCACTACGGata < 2:286684/150‑1 (MQ=255) gAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACTTGAATTGGCCAGGGCGCAGCAGGTAGTCGTCACTACGGATATAgg < 1:244261/150‑1 (MQ=255) aTGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGTGTTCGAACGGGATGTACTTGAATTGGCCAGGGCGCAGCAGGTAGTCGTCACTACGGATATAg < 2:537857/147‑1 (MQ=255) aTGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGTGTTCGAACGGGATGTACTTGAATTGGCCAGGGCGCAGCAGGTAGTCGTCACTACGGATATAg > 1:537857/1‑147 (MQ=255) ttGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACTTGAATTGGCCAGGGCGCAGCAGGTAgtc < 1:13762/120‑1 (MQ=255) ttGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACTTGAATTGGCCAGGGCGCAGCAGGTAgtc > 2:13762/1‑120 (MQ=255) cgcaAACTCTCGTTTTCATGGCCTCGATAGTCGGCGATCACCCCTACGAAGCGTATTTCTTCTTAGATCTGTTCTAACGTGATGTACTTTAATTCTCCAGGGCGCAGCAGGTAGTCGTCACTACGGATATAGGTACTTCGCAGCGCCTTg < 2:219287/150‑1 (MQ=255) cTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGGGTGGATGGGTTCGAACGAAATGTACTTGAATTGGCCCGGGCGGAGCAGTTAGTCGTCACTAAGGATATCGGTAGTGCGCAGCGCGTTTGctcgg > 2:91568/1‑146 (MQ=25) tGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGATCGAACGGGATGTACTTGAATTGGCCAGGGCGCAGCAGGTAGTCGTCACTACGGATATAGGTACTGCGCAGCGCCTTGGCCGGGt > 2:222779/1‑150 (MQ=255) cTCGGTCGTCAGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACTTGAATTGGCCAGGGCGCAGCAGGTAGTCGTCACTACGGATATAGGTACTGCGCAGCGCCTTGGCCGGGTCCTGCTCCAGTAGCt < 1:307078/150‑1 (MQ=255) cgacgaAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACTTTAATTGGCCAGGGCGCAGCAGGTAGTCGTCACTACGGATATAGGTACTGCGCAGCGCCTTGGCCGGGTCCTGCTCCAGTAGCTGGTACACGGTGTTGCgcagca < 1:16372/150‑1 (MQ=255) tGGATGTGTTCGAACGGGATGTACTTGAATTGGCCAGGGCGCAGCAGGTAGTCGTCACTACGGATATAGGTACTGCGCAGCGCCTTGGCCGGGTCCTGCTCCAGTAGCTGGTACACGGTGTTGCGCAGCAGTGAGTCGTCGCGCAGTTGc > 1:488450/1‑150 (MQ=255) | GATGTGCGTGTCGTTGACCAACACCGACAGCATGATCTGGCGGCCACGCGGCGGAATGCGCAGTACTTGCCGCCAACTGGCGTTTTCATGGCCTCGGTAGTCGGCGATCACCGCGACGAAGCGGGTTTCGGCGTGGATGGGTTCGAACGGGATGTACTTGAATTGGCCAGGGCGCAGCAGGTAGTCGTCACTACGGATATAGGTACTGCGCAGCGCCTTGGCCGGGTCCTGCTCCAGTAGCTGGTACACGGTGTTGCGCAGCAGTGAGTCGTCGCGCAGTTGC > NC_002947/2994531‑2994813 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |