Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,656,397 A→C L450R (CTG→CGG)  tktA ← transketolase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,656,3970AC99.2% 884.8 / ‑2.6 247L450R (CTG→CGG) tktAtransketolase A
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base C (111/134);  total (111/136)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 251 total aligned reads displayed.

TTCCACCGCGTCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCC  >  NC_002947/5656252‑5656542
                                                                                                                                                 |                                                                                                                                                 
ttCCACCGCGTCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGc                                                                                                                                               >  2:6564278/1‑150 (MQ=255)
 tCCACCGCGTCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGcc                                                                                                                                              <  1:6395417/150‑1 (MQ=255)
      cgcgTCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACAt                                                                                                                                         <  1:2440901/150‑1 (MQ=255)
        cgTCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATgc                                                                                                                                       <  1:3894205/150‑1 (MQ=255)
            ggCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCAc                                                                                                                                   >  1:4554361/1‑150 (MQ=255)
             gCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACg                                                                                                                                  >  2:4039226/1‑150 (MQ=255)
                    gcCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGc                                                                                                                           >  2:4821973/1‑150 (MQ=255)
                    gcCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGc                                                                                                                           <  2:2548282/150‑1 (MQ=255)
                      cAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCgg                                                                                                                         <  1:6333736/150‑1 (MQ=255)
                      cAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCgg                                                                                                                         <  2:1378057/150‑1 (MQ=255)
                       aGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCggg                                                                                                                        >  1:3824059/1‑150 (MQ=255)
                        ggTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGc                                                                                                                       <  1:850140/150‑1 (MQ=255)
                        ggTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGc                                                                                                                       <  1:5467905/150‑1 (MQ=255)
                        ggTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGc                                                                                                                       <  2:4544110/150‑1 (MQ=255)
                        ggTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGc                                                                                                                       <  2:4544018/150‑1 (MQ=255)
                        ggTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGc                                                                                                                       <  2:3862959/150‑1 (MQ=255)
                        ggTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGc                                                                                                                       <  2:303509/150‑1 (MQ=255)
                        ggTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGc                                                                                                                       <  2:1192855/150‑1 (MQ=255)
                          tgtCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGt                                                                                                                     <  1:6877599/150‑1 (MQ=255)
                          tgtCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGt                                                                                                                     <  1:1214368/150‑1 (MQ=255)
                             ccAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTAtt                                                                                                                  >  1:1595701/1‑150 (MQ=255)
                              cAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTc                                                                                                                 <  1:4183443/150‑1 (MQ=255)
                                 gTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCAt                                                                                                              <  1:6033038/150‑1 (MQ=255)
                                  ttCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATg                                                                                                             <  1:5823184/150‑1 (MQ=255)
                                   tCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGa                                                                                                            <  2:1258856/150‑1 (MQ=255)
                                   tCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGa                                                                                                            <  1:1232854/150‑1 (MQ=255)
                                   tCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGa                                                                                                            <  1:6055595/150‑1 (MQ=255)
                                    cGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGaa                                                                                                           <  2:942410/150‑1 (MQ=255)
                                        gTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATc                                                                                                       >  2:5248455/1‑150 (MQ=255)
                                         tgctgcGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCa                                                                                                      <  1:3175433/150‑1 (MQ=255)
                                             gcgcAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGaa                                                                                                  <  2:495191/150‑1 (MQ=255)
                                             gcgcAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGaa                                                                                                  >  2:780590/1‑150 (MQ=255)
                                               gcAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAgg                                                                                                >  1:1355154/1‑150 (MQ=255)
                                                 aGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTc                                                                                              <  1:6665567/150‑1 (MQ=255)
                                                   gCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTcgc                                                                                            >  1:1097527/1‑150 (MQ=255)
                                                     tGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCgcgc                                                                                          >  2:1448984/1‑150 (MQ=255)
                                                     tGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCgcgc                                                                                          <  1:6693004/150‑1 (MQ=255)
                                                      ggTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGcc                                                                                         <  1:5611541/150‑1 (MQ=255)
                                                      ggTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGcc                                                                                         <  1:3509724/150‑1 (MQ=255)
                                                        tCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGt                                                                                       >  2:5488586/1‑150 (MQ=255)
                                                        tCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGt                                                                                       >  1:1825841/1‑150 (MQ=255)
                                                           gctgctCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTTCACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAg                                                                                    <  2:2116377/150‑1 (MQ=255)
                                                           gctgctCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCACACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAg                                                                                    >  2:6605972/1‑150 (MQ=255)
                                                            ctgctCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAgg                                                                                   <  2:4313263/150‑1 (MQ=255)
                                                            ctgctCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAgg                                                                                   >  2:2405330/1‑150 (MQ=255)
                                                            ctgctCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAgg                                                                                   <  1:4477818/150‑1 (MQ=255)
                                                            ctgctCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAgg                                                                                   >  1:4823340/1‑150 (MQ=255)
                                                              gctCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCa                                                                                 <  1:3297445/150‑1 (MQ=255)
                                                                  gATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAg                                                                             >  2:3524899/1‑150 (MQ=255)
                                                                    tCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGc                                                                           >  2:6846845/1‑150 (MQ=255)
                                                                    tCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGc                                                                           <  1:2926008/150‑1 (MQ=255)
                                                                    tCGGCTGGTGCGTCGGGCCGTCTACGCCCAGAACGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCCGGCCGACATGCGCACGGCGTAGCGGGCGTATTCCATGAACATCAGGAACGTCGCGCCGTAACGCACCAGGc                                                                           >  2:5202088/1‑150 (MQ=255)
                                                                        cTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCAcc                                                                       <  1:6846845/150‑1 (MQ=255)
                                                                         tGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCg                                                                      >  2:5823549/1‑150 (MQ=255)
                                                                             gCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCa                                                                  <  2:2580528/150‑1 (MQ=255)
                                                                             gCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTAGTCCATGAACCTCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCa                                                                  <  2:4137763/150‑1 (MQ=255)
                                                                              cGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAg                                                                 <  2:5878675/150‑1 (MQ=255)
                                                                              cGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAg                                                                 <  1:2374647/150‑1 (MQ=255)
                                                                               gTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTAGTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAgg                                                                <  1:2144231/150‑1 (MQ=255)
                                                                                tCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAggg                                                               <  1:3345897/150‑1 (MQ=255)
                                                                                   ggCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCaa                                                            >  1:277312/1‑150 (MQ=255)
                                                                                   ggCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCaa                                                            >  2:1066977/1‑150 (MQ=255)
                                                                                    gCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAAc                                                           <  1:5574263/150‑1 (MQ=255)
                                                                                     ccGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACg                                                          >  2:6020576/1‑150 (MQ=255)
                                                                                     ccGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACg                                                          >  1:4452848/1‑150 (MQ=255)
                                                                                       gTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGcc                                                        <  2:33138/150‑1 (MQ=255)
                                                                                          ttCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGtt                                                     >  2:1638022/1‑150 (MQ=255)
                                                                                          ttCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGtt                                                     >  2:2750715/1‑150 (MQ=255)
                                                                                            cGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCa                                                   <  1:5019920/150‑1 (MQ=255)
                                                                                            cGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCa                                                   <  2:4446678/150‑1 (MQ=255)
                                                                                            cGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCGTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCa                                                   <  2:3793501/150‑1 (MQ=255)
                                                                                               ccAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCatga                                                >  1:5060984/1‑150 (MQ=255)
                                                                                               ccAGACCGATGGAGTCGGGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACTTCAGGGAGGTCGCGCGGTAAGGCACCAGGCCACCGTGCCGGGCAACGCCGTTCatgt                                                >  2:464256/1‑149 (MQ=255)
                                                                                                 aGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCatgatg                                              <  2:1316915/150‑1 (MQ=255)
                                                                                                     cGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCgg                                          >  2:5741682/1‑150 (MQ=255)
                                                                                                     cGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCgg                                          <  1:3758353/150‑1 (MQ=255)
                                                                                                      gATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGt                                         >  1:2042488/1‑150 (MQ=255)
                                                                                                      gATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGt                                         >  1:1007823/1‑150 (MQ=255)
                                                                                                      gATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGt                                         >  1:979273/1‑150 (MQ=255)
                                                                                                        tGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCa                                       >  2:6687896/1‑150 (MQ=255)
                                                                                                               gTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGaa                                >  1:4804157/1‑150 (MQ=255)
                                                                                                               gTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGaa                                <  2:2296491/150‑1 (MQ=255)
                                                                                                               gTGGGTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGaa                                <  2:4608074/150‑1 (MQ=255)
                                                                                                               gTGGGTGTACAAGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGaa                                >  2:3817639/1‑150 (MQ=255)
                                                                                                                  ggTGTACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTc                             <  1:881261/150‑1 (MQ=255)
                                                                                                                    tgtACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTcgc                           <  1:2078955/150‑1 (MQ=255)
                                                                                                                    tgtACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTcgc                           <  1:4360170/150‑1 (MQ=255)
                                                                                                                    tgtACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTcgc                           <  1:4766994/150‑1 (MQ=255)
                                                                                                                      tACACGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCgcgc                         <  2:6667445/150‑1 (MQ=255)
                                                                                                                         acGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACg                      >  2:1881663/1‑150 (MQ=255)
                                                                                                                          cGTGGATCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGc                     >  1:5193246/1‑150 (MQ=255)
                                                                                                                                tCACGCGCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGa               >  2:6212404/1‑150 (MQ=255)
                                                                                                                                   cgcgCTGCTTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAaca            <  2:4216771/150‑1 (MQ=255)
                                                                                                                                   cgcgCTGCCTCATCCGGGCCGACATGCGCACGGCGTGGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCCGGCCACCGTGCAGGGCAACGCCGTTCACGATGGCGGTCAAGCCGAACTCGCGCACGCCGTAGAaca            <  2:1901192/150‑1 (MQ=255)
                                                                                                                                      gctgctTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGt         >  1:5338961/1‑150 (MQ=255)
                                                                                                                                        tgctTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAg       <  2:1613889/150‑1 (MQ=255)
                                                                                                                                          ctTCATCCGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGtt     <  1:1911589/150‑1 (MQ=255)
                                                                                                                                             catccGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTCGTTGcc  >  1:2214386/1‑150 (MQ=255)
                                                                                                                                             catccGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGcc  >  2:3610336/1‑150 (MQ=255)
                                                                                                                                             catccGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGcc  >  1:1476888/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
TTCCACCGCGTCGGCCGGGCGCCAGGTGTCCAGGTTCGGGGTGCTGCGCAGGCTGGTCAGCTGCTCGATCGGCTGGTGCGTCGGGCCGTCTTCGCCCAGACCGATGGAGTCGTGGGTGTACACGTGGATCACGCGCTGCTTCATCAGGGCCGACATGCGCACGGCGTTGCGGGCGTATTCCATGAACATCAGGAAGGTCGCGCCGTAAGGCACCAGGCCACCGTGCAGGGCAACGCCGTTCATGATGGCGGTCATGCCGAACTCGCGCACGCCGTAGAACACGTAGTTGCC  >  NC_002947/5656252‑5656542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑