Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,852,053 G→T W366L (TGG→TTG)  relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,852,0530GT99.7% 1097.4 / NA 302W366L (TGG→TTG) relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (164/137);  total (164/138)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

AGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACC  >  NC_002947/1851906‑1852199
                                                                                                                                                   |                                                                                                                                                  
aGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTgc                                                                                                                                                  <  1:6582772/150‑1 (MQ=255)
     gacgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTAc                                                                                                                                             >  2:1740588/1‑150 (MQ=255)
      acgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTGCa                                                                                                                                            <  2:3878339/150‑1 (MQ=255)
      acgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACa                                                                                                                                            >  1:1652511/1‑150 (MQ=255)
      acgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACa                                                                                                                                            >  2:2855498/1‑150 (MQ=255)
       cgacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACaa                                                                                                                                           <  2:5222792/150‑1 (MQ=255)
       agaatACATCGCCAACCCCTAGGAGAACGGCTACCTCTCGTTGCACACTGCGTTAATCGTCAACTAGGGCAAGGTTCTGGAGGTGCATATACGTAACCACGTCATGCAATAAGATTCCTAACTTGGCGTATGCGCCCACTTGCTCTACaa                                                                                                                                           <  2:3485797/146‑1 (MQ=255)
         acTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAgg                                                                                                                                         <  2:1508371/150‑1 (MQ=255)
         acTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAgg                                                                                                                                         >  2:2137087/1‑150 (MQ=255)
         acTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAgg                                                                                                                                         <  1:2994781/150‑1 (MQ=255)
          cTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAggg                                                                                                                                        >  1:937096/1‑150 (MQ=255)
           tACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGc                                                                                                                                       >  1:5878095/1‑150 (MQ=255)
           tACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGc                                                                                                                                       >  1:4934951/1‑150 (MQ=255)
           tACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGc                                                                                                                                       >  1:4082917/1‑150 (MQ=255)
            aCATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCa                                                                                                                                      <  1:705777/150‑1 (MQ=255)
             cATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCAc                                                                                                                                     <  2:3672486/150‑1 (MQ=255)
              aTCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCAcc                                                                                                                                    <  2:1651466/150‑1 (MQ=255)
              aTCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCAcc                                                                                                                                    >  1:4464097/1‑150 (MQ=255)
               tCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCg                                                                                                                                   >  2:5507450/1‑150 (MQ=255)
                 gCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGAc                                                                                                                                 >  1:1449928/1‑150 (MQ=255)
                      ccccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCaa                                                                                                                            <  2:4481664/150‑1 (MQ=255)
                        ccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGc                                                                                                                          >  2:6157494/1‑150 (MQ=255)
                        ccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGc                                                                                                                          >  1:2678084/1‑150 (MQ=255)
                          aaGGAGAACGGCTACCGCTCGTTGCACAATGAGGTAATCGTCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCAGTACCCACGGCATGCAAGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGAGCTACAAGGGCACCGACGTCAAGcca                                                                                                                        >  1:4611228/1‑149 (MQ=255)
                             gagaACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGc                                                                                                                     >  2:2633635/1‑150 (MQ=255)
                               gaACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCTCTACAAGGGCACCGACGTCAAGCCCAGCTc                                                                                                                   >  2:3706622/1‑150 (MQ=255)
                                 aCGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCa                                                                                                                 >  1:6158650/1‑150 (MQ=255)
                                  cGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCaa                                                                                                                >  2:4188444/1‑150 (MQ=255)
                                   ggCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAAc                                                                                                               >  1:242980/1‑150 (MQ=255)
                                    gCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAAcc                                                                                                              >  1:3830736/1‑150 (MQ=255)
                                      tACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCAc                                                                                                            >  2:2987182/1‑150 (MQ=255)
                                         cGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTAc                                                                                                         >  2:6799158/1‑150 (MQ=255)
                                          gCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACg                                                                                                        <  2:6593721/150‑1 (MQ=255)
                                               ttGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaagaa                                                                                                   >  1:6527423/1‑150 (MQ=255)
                                                 gCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaaa                                                                                                 >  1:4546188/1‑150 (MQ=255)
                                                 gCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaaa                                                                                                 >  1:1304949/1‑150 (MQ=255)
                                                  cacaCTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAg                                                                                                >  2:1540911/1‑150 (MQ=255)
                                                   acacTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGa                                                                                               >  2:4958400/1‑150 (MQ=255)
                                                    cacTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGAt                                                                                              <  2:3603138/150‑1 (MQ=255)
                                                    cacTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGAt                                                                                              <  2:1113730/150‑1 (MQ=255)
                                                     acTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGAtt                                                                                             >  1:2051091/1‑150 (MQ=255)
                                                     acTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGAtt                                                                                             >  1:635517/1‑150 (MQ=255)
                                                       tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                                                                                           >  1:5737865/1‑150 (MQ=255)
                                                       tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                                                                                           >  1:2312581/1‑150 (MQ=255)
                                                        gCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTcc                                                                                          <  2:689556/150‑1 (MQ=255)
                                                         cGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCt                                                                                         >  2:4772069/1‑150 (MQ=255)
                                                          ggTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTg                                                                                        <  1:3441608/150‑1 (MQ=255)
                                                           gTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTgg                                                                                       <  1:6803730/150‑1 (MQ=255)
                                                               tCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGc                                                                                   <  1:4098213/150‑1 (MQ=255)
                                                                cGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCg                                                                                  >  1:5311259/1‑150 (MQ=255)
                                                                 ggCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGt                                                                                 >  1:777912/1‑150 (MQ=255)
                                                                  gCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTc                                                                                <  1:1546219/150‑1 (MQ=255)
                                                                  gCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTc                                                                                >  2:3784493/1‑150 (MQ=255)
                                                                  gCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTc                                                                                <  1:4455380/150‑1 (MQ=255)
                                                                  gCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTc                                                                                >  1:5057602/1‑150 (MQ=255)
                                                                     ccGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAgg                                                                             <  1:1392612/150‑1 (MQ=255)
                                                                         gggCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCt                                                                         <  1:433931/150‑1 (MQ=255)
                                                                            cAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGa                                                                      <  2:1677604/150‑1 (MQ=255)
                                                                              aGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGt                                                                    <  1:4249943/150‑1 (MQ=255)
                                                                               ggTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTg                                                                   >  2:1574617/1‑150 (MQ=255)
                                                                                gTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTgg                                                                  >  2:573270/1‑150 (MQ=255)
                                                                                  gCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCa                                                                >  1:3715274/1‑150 (MQ=255)
                                                                                  gCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCa                                                                <  2:3317429/150‑1 (MQ=255)
                                                                                   cTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCAc                                                               <  2:6500473/150‑1 (MQ=255)
                                                                                   cTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCAc                                                               >  2:2888625/1‑150 (MQ=255)
                                                                                       aGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAag                                                           <  1:6605340/150‑1 (MQ=255)
                                                                                         gTGCAGACCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAagag                                                         >  2:2190564/1‑150 (MQ=255)
                                                                                           gCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCt                                                       >  2:2319628/1‑150 (MQ=255)
                                                                                           gCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCt                                                       >  2:454279/1‑150 (MQ=255)
                                                                                           gCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCt                                                       >  2:1961485/1‑150 (MQ=255)
                                                                                             aGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTgg                                                     <  1:352441/150‑1 (MQ=255)
                                                                                             aGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTgg                                                     >  1:2621477/1‑150 (MQ=255)
                                                                                              gATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTggg                                                    <  2:3957969/150‑1 (MQ=255)
                                                                                                    tACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACAt                                              >  1:2158209/1‑150 (MQ=255)
                                                                                                       ccACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCgg                                           >  2:3634941/1‑150 (MQ=255)
                                                                                                        cACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGt                                          <  2:2084273/150‑1 (MQ=255)
                                                                                                        cACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGt                                          <  1:4690464/150‑1 (MQ=255)
                                                                                                              aTGCACGAAGAGGCCGTACTTGGCGTATGCGCACACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtggcctg                                    <  1:5201449/150‑1 (MQ=255)
                                                                                                                gCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtggcctggc                                  <  1:4188482/150‑1 (MQ=255)
                                                                                                                gCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGtggcctggc                                  >  2:2484973/1‑150 (MQ=255)
                                                                                                                  aCGAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCg                                >  2:1270042/1‑150 (MQ=255)
                                                                                                                    gAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAg                              >  2:1480049/1‑150 (MQ=255)
                                                                                                                    gAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAg                              >  1:3397685/1‑150 (MQ=255)
                                                                                                                    gAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAg                              <  1:6239805/150‑1 (MQ=255)
                                                                                                                    gAAGAGGCCGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAg                              <  1:5135641/150‑1 (MQ=255)
                                                                                                                           ccGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGc                       <  2:4659399/150‑1 (MQ=255)
                                                                                                                           ccGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGc                       <  2:5951564/150‑1 (MQ=255)
                                                                                                                           ccGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGc                       <  1:2226006/150‑1 (MQ=255)
                                                                                                                            cGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGTTTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCg                      >  2:5844457/1‑150 (MQ=255)
                                                                                                                            cGAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCg                      >  1:2872640/1‑150 (MQ=255)
                                                                                                                             gAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCgg                     >  1:5610367/1‑150 (MQ=255)
                                                                                                                             gAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCgg                     >  2:6617306/1‑150 (MQ=255)
                                                                                                                             gAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCgg                     <  2:3129225/150‑1 (MQ=255)
                                                                                                                             gAACTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCgg                     <  1:3217011/150‑1 (MQ=255)
                                                                                                                                cTTGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTc                  <  1:4468570/150‑1 (MQ=255)
                                                                                                                                 ttGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCg                 >  1:4755690/1‑150 (MQ=255)
                                                                                                                                 ttGGCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCg                 >  1:1144234/1‑150 (MQ=255)
                                                                                                                                   ggCGTATGCGCCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGAc               >  2:1347460/1‑150 (MQ=255)
                                                                                                                                            gcCCACTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCt      >  2:504298/1‑150 (MQ=255)
                                                                                                                                                aCTTGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGAcc  >  1:1055151/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCGACATCGGTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACC  >  NC_002947/1851906‑1852199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑