Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,295,031:1	+T	29.6%	intergenic (+41/+29)	slyB → / ← nhaA‑I	outer membrane lipoprotein/Na(+)/H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,295,031	1	.	T	29.6%	46.4 / 30.4	27	intergenic (+41/+29)	slyB/nhaA‑I	outer membrane lipoprotein/Na(+)/H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base . (11/8);  new base T (4/4);  total (15/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGCTACCCGGG  >  NC_002947/1294882‑1295180                                                                                                                                                       |                                                                                                                                                      tgGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCa                                                                                                                                                        >  2:204477/1‑150 (MQ=255)       caTGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGAATGTAGACGGTACCAGTCGCGTTTCGCACTGACAGTAGAAAAAAAAAAAAAAACCCCGAC‑TGGCAGCAGCCATGTCggg                                                                                                                                                 <  2:21915/150‑1 (MQ=255)            gcgcCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGtttt                                                                                                                                            <  1:502442/150‑1 (MQ=255)                  aTGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATg                                                                                                                                      <  2:517399/150‑1 (MQ=255)                                         gAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCt                                                                                                               >  1:477833/1‑150 (MQ=255)                                               ttCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGtttt                                                                                                         >  2:458672/1‑150 (MQ=255)                                                                   gcgcATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGc                                                                                                    >  1:374222/1‑135 (MQ=255)                                                                   gcgcATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGc                                                                                                    <  2:374222/135‑1 (MQ=255)                                                                               tGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGa                                                                         <  1:458672/150‑1 (MQ=255)                                                                                   gACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGCCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCAc                                                                     >  1:158833/1‑150 (MQ=255)                                                                                    aCGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCAcc                                                                    >  2:485287/1‑150 (MQ=255)                                                                                        tACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGGTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCgcag                                                                >  1:43698/1‑150 (MQ=255)                                                                                                        cACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGt                                                <  1:204477/150‑1 (MQ=255)                                                                                                         aCTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTa                                               >  2:338931/1‑150 (MQ=255)                                                                                                          cTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGttt                                                                                                          >  2:573036/1‑90 (MQ=255)                                                                                                          cTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGttt                                                                                                          <  1:573036/90‑1 (MQ=255)                                                                                                                       aGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCg                                 >  2:298593/1‑150 (MQ=255)                                                                                                                         taataaAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGt                               <  1:338931/150‑1 (MQ=255)                                                                                                                          atgaaaaaaCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAgaa                                                         <  2:194916/120‑1 (MQ=37)                                                                                                                          atgaaaaaaCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAgaa                                                         >  1:194916/4‑123 (MQ=37)                                                                                                                             aaaaaaCCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGCCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCAt                           <  2:158833/150‑1 (MQ=255)                                                                                                                                aaaCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGcc                        >  1:394478/1‑150 (MQ=39)                                                                                                                                 aaCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACACAAGAAAACAACGACCCACGAAGAATTCCCATGCGGGCCATGCCg                       >  2:440178/1‑150 (MQ=255)                                                                                                                                         actGGCAGCGGCCATGTCGGGGTGTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGt                                                                                       <  1:261535/77‑1 (MQ=255)                                                                                                                                         actGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGt                                                                                       >  2:261535/3‑79 (MQ=37)                                                                                                                                         actGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACt                <  2:394478/148‑1 (MQ=255)                                                                                                                                             gctgctGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTg           >  2:276331/1‑150 (MQ=255)                                                                                                                                                 ctgcCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGCTAc       >  2:19861/1‑150 (MQ=255)                                                                                                                                                      a‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGCTACCCggg  <  1:298593/150‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                      TGGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGCTACCCGGG  >  NC_002947/1294882‑1295180

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑