| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,295,027 | T→A | 27.3% | intergenic (+37/+33) | slyB → / ← nhaA‑I | outer membrane lipoprotein/Na(+)/H(+) antiporter NhaA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,295,027 | 0 | T | A | 27.3% | 41.1 / 12.1 | 26 | intergenic (+37/+33) | slyB/nhaA‑I | outer membrane lipoprotein/Na(+)/H(+) antiporter NhaA |
| Reads supporting (aligned to +/- strand): ref base T (10/8); new base A (4/3); total (14/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GATGGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGCTAC > NC_002947/1294880‑1295175 | gatgGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCtgctgc > 2:502442/1‑150 (MQ=255) tgGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCa > 2:204477/1‑150 (MQ=255) caTGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGAATGTAGACGGTACCAGTCGCGTTTCGCACTGACAGTAGAAAAAAAAAAAAAAACCCCGAC‑TGGCAGCAGCCATGTCggg < 2:21915/150‑1 (MQ=255) gcgcCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGtttt < 1:502442/150‑1 (MQ=255) aTGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATg < 2:517399/150‑1 (MQ=255) ggAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCtgctgc < 1:210772/115‑1 (MQ=255) ggAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCtgctgc > 2:210772/1‑115 (MQ=255) gAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCt > 1:477833/1‑150 (MQ=255) ttCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGtttt > 2:458672/1‑150 (MQ=255) gcgcATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGc < 2:374222/135‑1 (MQ=255) gcgcATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGc > 1:374222/1‑135 (MQ=255) tGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGa < 1:458672/150‑1 (MQ=255) gACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGCCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCAc > 1:158833/1‑150 (MQ=255) aCGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCAcc > 2:485287/1‑150 (MQ=255) tACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGGTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCgcag > 1:43698/1‑150 (MQ=255) cACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGt < 1:204477/150‑1 (MQ=255) aCTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTa > 2:338931/1‑150 (MQ=255) cTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGttt < 1:573036/90‑1 (MQ=255) cTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGttt > 2:573036/1‑90 (MQ=255) aGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCg > 2:298593/1‑150 (MQ=255) taataaAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGt < 1:338931/150‑1 (MQ=255) atgaaaaaaCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAgaa > 1:194916/4‑123 (MQ=37) atgaaaaaaCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAgaa < 2:194916/120‑1 (MQ=37) aaaaaaCCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGCCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCAt < 2:158833/150‑1 (MQ=255) aaaCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGcc > 1:394478/1‑150 (MQ=39) aaCCCCGAC‑TGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACACAAGAAAACAACGACCCACGAAGAATTCCCATGCGGGCCATGCCg > 2:440178/1‑150 (MQ=255) actGGCAGCGGCCATGTCGGGGTGTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGt < 1:261535/77‑1 (MQ=255) actGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGt > 2:261535/3‑79 (MQ=37) actGGCAGCAGCCATGTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACt < 2:394478/148‑1 (MQ=255) gctgctGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTg > 2:276331/1‑150 (MQ=255) ctgcCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGCTAc > 2:19861/1‑150 (MQ=255) | GATGGCAGCATGCGCGCCTATGTGCAGGCCGTGCAGGAGAACGAAATCTTCCGTGTTGGCGACCGAGTGCGCATCATGACTGTAGACGGTACCAGTCGCGTTTCGCACTGACCGTAGTGCAGTAATAAAAAACCCCGACATGGCTGCTGCCA‑GTCGGGGTTTTTTCATGCCTCAGCCAAGTCAGGCAATGCTGGTTTTGCGGCTGGCCATTGCGGTGACGGCGTAGCCGATCACCGCAGCAAAGAACGAACCAGTAAGAATGCCCATGCGGTCCATGCCGGCGTACTCACTGCTAC > NC_002947/1294880‑1295175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |