Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,337,594 T→G 18.8% L102V (TTG→GTG)  PP_1163 → FAD‑binding oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,337,5940TG18.8% 58.8 / 11.9 32L102V (TTG→GTG) PP_1163FAD‑binding oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (17/9);  new base G (3/3);  total (20/12)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.94e-01

ACCGTTCATGGTCGCCGCCAGTGTGGATGCCCAGGGTCGGCCTTGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAGGGCAACTGCAAGTGGCCGTAGAGCAGGCGTTTGGCAACTGCCCG  >  NC_002947/1337446‑1337731
                                                                                                                                                    |                                                                                                                                         
cccGTTCATGGTCGCCGCCAGTGTGGATGCCCAGGGTCGGCCTTGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGtt                                                                                                                                          <  2:107814/149‑1 (MQ=255)
                              ccAGGGTCGGCCTTGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTggg                                                                                                            >  1:276255/1‑150 (MQ=255)
                              ccAGGGTCGGCCTTGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTggg                                                                                                            >  1:468541/1‑150 (MQ=255)
                               cAGGGTCGGCCTTGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTggg                                                                                                            >  1:308670/1‑149 (MQ=255)
                               cAGGGTCGGCCTTGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTggg                                                                                                            <  2:308670/149‑1 (MQ=255)
                                     cGGCCTTGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGaa                                                                                                     <  2:591943/150‑1 (MQ=255)
                                       gCCTTGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAATCTCGCCTGACCATCGTATGCTGACGATCCACAGCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGGCGGCCTGCTGGTGATCGAACt                                                                                                   >  1:152912/1‑150 (MQ=255)
                                       gCCTTGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACGCTCGTATGCTGACGATCCACACCACGTGGCCAGCAGATGACCCCGCCACCCCCGGATTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACt                                                                                                   >  1:593037/1‑150 (MQ=255)
                                       gCCTTGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACt                                                                                                   >  2:331494/1‑150 (MQ=255)
                                       gCCTTGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGGCGGCCTGCTGGGGATCGAACt                                                                                                   >  1:152950/1‑150 (MQ=255)
                                          ttGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGca                                                                                                >  2:148491/1‑150 (MQ=255)
                                             ggCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCacac                                                                                             >  2:603158/1‑150 (MQ=255)
                                                                 cAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCa                                                                         >  2:179220/1‑150 (MQ=255)
                                                                   gAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTggg                                                                                                            <  1:112212/113‑1 (MQ=255)
                                                                   gAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTggg                                                                                                            >  2:112212/1‑113 (MQ=255)
                                                                               aGCTCGCCTGCCCCTCGTATGCTGCCGCTACACACCCCGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGc                                                           <  2:468541/150‑1 (MQ=255)
                                                                                gCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGcc                                                          >  2:327291/1‑150 (MQ=255)
                                                                                    gCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGGCCTTCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGc                                                      >  1:375259/1‑150 (MQ=255)
                                                                                     ccTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACg                                                                      >  2:98777/1‑133 (MQ=255)
                                                                                     ccTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACg                                                                      <  1:98777/133‑1 (MQ=255)
                                                                                              cGTATGCTGACTATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCACCAACGGGCAGATCCGCCACGCTGCCCAAggg                                            <  2:276255/150‑1 (MQ=255)
                                                                                                     tGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAGGGCAACTGc                                     >  1:525917/1‑150 (MQ=255)
                                                                                                         gATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAGGGCAACTGCAAGt                                 >  1:610163/1‑150 (MQ=255)
                                                                                                             cacaCCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAGGGCAACTGCAAGTGGcc                             <  2:610163/150‑1 (MQ=255)
                                                                                                                accacGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAGGGCAACTGCAAGTGGCCGTa                          >  1:137549/1‑150 (MQ=255)
                                                                                                                  cacGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAGGGCAACTGCAAGTGGCCGTaga                        <  1:603158/150‑1 (MQ=255)
                                                                                                                                  ccggctgCCAACCCGGGGGTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAGGGCAACTGCAAGTGGCCGTAGAGCAGGCGTTTGGCAAc        >  2:1694/7‑150 (MQ=255)
                                                                                                                                     gctgCCAACCCGGGGGTGGCGGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAgg                                             <  2:203492/107‑1 (MQ=255)
                                                                                                                                     gctgCCAACCCGGGGGTGGCGGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAgg                                             <  2:203543/107‑1 (MQ=255)
                                                                                                                                     gctgCCAACCCGGGGGTGGCGGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAgg                                             >  1:203543/4‑110 (MQ=255)
                                                                                                                                     gctgCCAACCCGGGGGTGGCGGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAgg                                             >  1:203492/4‑110 (MQ=255)
                                                                                                                                     gctgCCAACCCGGGGGTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAGGGCAACTGCAAGTGGCCGTAGAGCAGGCGTTTGGCAACTGc     <  1:1694/147‑1 (MQ=255)
                                                                                                                                        gCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAGGGCAACTGCAAGTGGCCGTAGAGCAGGCGTTTGGCAACTGCCCg  <  2:525917/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                         
ACCGTTCATGGTCGCCGCCAGTGTGGATGCCCAGGGTCGGCCTTGGGCCACGCTGCTGGAAGGGCCAGAAGGGTTCGTAAGCTCGCCTGACCCTCGTATGCTGACGATCCACACCACGTTGCCAGCAGATGACCCCGCCACCCCCGGGTTGGCAGCCGGGCAGGCGGTCGGCCTGCTGGGGATCGAACTGCACACCCGTCGGCGCAATCGCATCAACGGGCAGATCCGCCACGCTGCCCAAGGGCAACTGCAAGTGGCCGTAGAGCAGGCGTTTGGCAACTGCCCG  >  NC_002947/1337446‑1337731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑