| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,392,049 | T→C | 15.4% | intergenic (+74/‑41) | aspS → / → PP_1214 | aspartate‑‑tRNA ligase/transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,392,049 | 0 | T | C | 15.4% | 58.4 / 4.0 | 26 | intergenic (+74/‑41) | aspS/PP_1214 | aspartate‑‑tRNA ligase/transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (11/11); new base C (2/2); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.99e-01 | |||||||||||
CTGGCATGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACG > NC_002947/1391905‑1392188 | cTGGCATGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTGCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATggggc > 2:178341/1‑150 (MQ=255) tCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATggggcggg < 2:287801/144‑1 (MQ=255) tCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATggggcggg > 1:287801/1‑144 (MQ=255) gCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCaaa < 1:154705/150‑1 (MQ=255) gCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGt < 1:178341/150‑1 (MQ=255) cacaTCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGtt > 2:349611/1‑150 (MQ=255) aGCAGTCCCCGGGCGCATCCACATGGGTGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGCCCCGCCCCATGACggggcgggacg > 2:386352/1‑83 (MQ=25) aGCAGTCCCCGGGCGCATCCACATGGGTGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGCCCCGCCCCATGACggggcgggacg < 1:386352/86‑4 (MQ=25) gCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGAATCGGTGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCAc > 1:439678/1‑150 (MQ=255) gTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGc < 1:547524/150‑1 (MQ=255) gggCGCATCCACATGGATGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGGTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATAAAGCACCGCAAagag > 2:237723/1‑150 (MQ=255) acaTGGATGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATg > 2:112699/1‑150 (MQ=255) aTGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAg > 1:234426/1‑75 (MQ=255) aTGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAg < 2:234426/75‑1 (MQ=255) tGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAagag > 2:198775/1‑150 (MQ=255) ttGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAg < 1:412647/150‑1 (MQ=255) gACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGTGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGATAGGCAAGGTCTTCAcc > 1:252044/1‑150 (MQ=255) cAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGt > 2:292647/1‑150 (MQ=255) cAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGt > 2:68709/1‑150 (MQ=255) cAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTAATGGCTCCTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGt < 1:230266/150‑1 (MQ=255) ggTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTgg < 1:112699/150‑1 (MQ=255) ttttttCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTGGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGAt < 1:73657/150‑1 (MQ=255) ccgtcccgCCCCGTCATGGGGCGGTGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGATAGGCAAGGTCTTCACCAAGTGGATCCGTGaa < 2:252044/150‑1 (MQ=255) cccgCCCCATGACGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCaa < 1:484397/112‑1 (MQ=255) cccgCCCCATGACGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCaa > 2:484397/1‑112 (MQ=255) cgCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACg < 1:198775/150‑1 (MQ=255) | CTGGCATGGTCGACGCCAAGGCCCTGCGCGAACTGCACATCCGACTGCGCGAACAGACCAAGGTCGAGTAAGCAGTCCCCGGGCGCATCCACATGGATGCGCCTTTGCGTTTCGACGCAGGTTTTTTCCCGTCCCGCCCCGTCATGGGGCGGGGCCGTTTGTGATTCAAAGGATTCGGAGTCGTTATGGCTGGTCATTCCAAGTGGGCGAACATCAAGCACCGCAAAGAGCGCCAGGATGCCAAGAGAGGCAAGGTCTTCACCAAGTGGATCCGTGAACTGACG > NC_002947/1391905‑1392188 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |