Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	1,564,973	A→T	23.4%	L133Q (CTG→CAG)	pitB ←	phosphate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,564,973	0	A	T	23.4%	40.6 / 6.7	21	L133Q (CTG→CAG)	pitB	phosphate transporter
Reads supporting (aligned to +/- strand):  ref base A (9/7);  new base T (2/3);  total (11/10)
Fisher's exact test for biased strand distribution p-value = 6.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTG  >  NC_002947/1564850‑1565104                                                                                                                            |                                                                                                                                    aGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACCCCGAGGATGGAACCGATc                                                                                                           >  2:168949/1‑150 (MQ=255)    aCCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCTGGCCAacgc                                                                                                                            >  2:467652/1‑128 (MQ=255)    aCCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCTGGCCAacgc                                                                                                                            <  1:467652/130‑3 (MQ=255)    aCCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGc                                                                                                        <  1:134256/150‑1 (MQ=255)      cAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCTGTGATCAGCGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCgt                                                                                                      >  2:420286/1‑150 (MQ=255)                       aGCCGGCCATTGGCGAGCCCCCCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGACTGTCGTTGATCCGGTGGAACTCCTGTTCAGTACCTAGTATGGATCCGATCAGCGTGTGCGAACTGGAGGCgg                                                                                     <  1:168949/150‑1 (MQ=255)                             ccATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTCACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGTCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGc                                                                               <  1:306624/150‑1 (MQ=255)                                      agaCCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTAcc                                                                      <  2:322313/150‑1 (MQ=255)                                            ccaGCGACATGGCGATATTGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGc                                                                >  2:55058/1‑150 (MQ=255)                                                   cATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGtt                                                         >  1:246827/1‑150 (MQ=255)                                                                                 gACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGagaa                           >  2:265205/1‑150 (MQ=255)                                                                                   cGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGTCGCAGCCAGCAACGagaaga                         >  1:466516/1‑150 (MQ=255)                                                                                    gCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGca                                         >  1:274510/1‑133 (MQ=255)                                                                                    gCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGca                                         <  2:274510/133‑1 (MQ=255)                                                                                     ccGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGAcc                       <  1:265205/150‑1 (MQ=255)                                                                                                       tCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCg     >  1:255307/1‑150 (MQ=255)                                                                                                         gTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGgt   >  2:575731/1‑150 (MQ=255)                                                                                                          ttGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGgtg  <  1:572341/150‑1 (MQ=255)                                                                                                          ttGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGgtg  <  1:55058/150‑1 (MQ=255)                                                                                                                tcggtgTTGGCCTGGCCAACGCCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATg                    >  2:297856/6‑126 (MQ=255)                                                                                                                tcggtgTTGGCCTGGCCAACGCCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATg                    <  1:297856/121‑1 (MQ=255)                                                                                                                            |                                                                                                                                    AGCACCAGGGCAGCAACGGCGAAGCCGGCCATTGGCGAGACCACCAGCGACATGGCGATATCGATCGCTTTCTGCCAGTTGACGCCGTCGCCCAGCGGAATGTCGTTGATCAGGGCGTTGGCCAGGCCAACACCGAGGATGGAACCGATCAGCGTGTGCGAACTGGAGGCGGGGATGCCAAAGTACCAGGTGCCCAGGTTCCAGGTGATGGCCGCAGCCAGCAACGAGAAGACCATGGCCAGGCCGTGGCCGGTG  >  NC_002947/1564850‑1565104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑