| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,603,504 | G→A | 12.5% | A317V (GCC→GTC) | PP_1406 ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,603,504 | 0 | G | A | 12.5% | 78.2 / 3.9 | 32 | A317V (GCC→GTC) | PP_1406 | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (13/15); new base A (2/2); total (15/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01 | |||||||||||
GGGTGCAGGAGCATTGCCTGGAAACGTTGTTGCGCGCGGATGGGTTGCTCTGACGCCACCCACCGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCTGATTGATCACCGAAGCCTCGCCCAGCGGCCGAATGGCAATGGATAGCTGTTGCGGACGGGCTTGGCGCACAGCGTC > NC_002947/1603358‑1603643 | gggTGCAGGAGCATTGCCTGGAAACGTTGTTGCGCGCGGATGGGTTGCTCTGACGCCACCCACCGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCAt < 1:203025/150‑1 (MQ=255) gAGCATTGCCTGGAAACGTTGTTGCGCGCGGATGGGTTGCTCTGACGCCACCCACCGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGaaa > 2:17714/1‑150 (MQ=255) aTTGCCTGGAAACGTTGTTGCGCGCGGATGGGTTGCTCTGACGCCACCCACCGGCACGCCTCCGGGGATGCTGTCAGAACTGGCCAGGTGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCa > 1:38555/1‑150 (MQ=255) tGGAAACGTTGTTGCGCGCGGATGGGTTGCTCTGACGCCACCCACCGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCAccgccg > 2:61626/1‑150 (MQ=255) gTTGCTCTGACGCCACCCACCGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCgaa > 1:419508/1‑111 (MQ=255) gTTGCTCTGACGCCACCCACCGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCgaa < 2:419508/111‑1 (MQ=255) aCGCCACCCACCGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAAtg > 1:547974/1‑150 (MQ=255) cacccaccGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAAtgctgc < 2:38555/150‑1 (MQ=255) ccaccGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCAccgcc > 2:531586/1‑108 (MQ=255) ccaccGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCAccgcc < 1:531586/108‑1 (MQ=255) ccaccGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTc < 1:437840/150‑1 (MQ=255) ccaccGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTc > 1:456856/1‑150 (MQ=255) caccGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCt < 2:547974/150‑1 (MQ=255) ccGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCtga < 1:371857/150‑1 (MQ=255) ggCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCCAAATTCTTCTTGttatt > 2:496711/1‑150 (MQ=255) ggCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCtgatt < 1:133958/150‑1 (MQ=255) gCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCtgattg < 2:252492/150‑1 (MQ=255) cAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCTGATTGATCACCGAAGCCTCGCCCAGCGGCCGAATgg < 2:456856/150‑1 (MQ=255) cAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCTGATTGATCACCGAAGCCTCGCCCAGCGGCCGAATgg < 1:17714/150‑1 (MQ=255) cAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCTGATTGATCACCGAAGCCTCGCCCAGCGGCCGAATgg > 1:265516/1‑150 (MQ=255) gggCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCTGATTGATCACCGAAGCCTCGCCCAGCGGCCGAATGGCAAt < 2:336912/150‑1 (MQ=255) gggCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCTGATTGATCACCGAAGCCTCGCCCAGCGGCCGAATGGCAAt < 1:61626/150‑1 (MQ=255) ttCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCtgat < 1:539236/107‑1 (MQ=255) ttCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCtgat > 2:539236/1‑107 (MQ=255) gcctgccCCAACGGGTGGGTGCTGACATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTc > 2:193572/1‑87 (MQ=255) gcctgccCCAACGGGTGGGTGCTGACATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTc > 2:193439/1‑87 (MQ=255) gcctgccCCAACGGGTGGGTGCTGACATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTc < 1:193572/87‑1 (MQ=255) gcctgccCCAACGGGTGGGTGCTGACATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTc < 1:193439/87‑1 (MQ=255) cAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCTGATTGATCACCGAAGCCTCGCCCAGCGGCCGAATGGCAATGGATAGCTGTTGCGGACGGGCTTGGCGcac > 2:168517/1‑150 (MQ=255) gtgggtgCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCTGATTGATCACCGAAGCCTCGCCCAGCGGCCGAATGGCAATGGATAGCTGTTGCGGACGGGCTTGGCGCACAGCGTc > 2:398088/1‑150 (MQ=255) tgCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGAt > 2:268893/1‑51 (MQ=255) tgCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGAt < 1:268893/51‑1 (MQ=255) | GGGTGCAGGAGCATTGCCTGGAAACGTTGTTGCGCGCGGATGGGTTGCTCTGACGCCACCCACCGGCACGCCTCCGGGGATGCTGTCAGCGCCGAGCAGAGGGCACTTCGATAGCCAATGCTGCCTGCCCCAACGGGTGGGTGCTGGCATCGAAGAAATGCACCGCCGTCCCCGGCAAATCCCCGAAACGATCTGCAAAATGCTGCTTCTGATTGATCACCGAAGCCTCGCCCAGCGGCCGAATGGCAATGGATAGCTGTTGCGGACGGGCTTGGCGCACAGCGTC > NC_002947/1603358‑1603643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |