| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 1,637,368 | A→T | 22.8% | intergenic (+35/+31) | pdxJ → / ← PP_1437 | pyridoxine 5'‑phosphate synthase/heavy metal sensor histidine kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,637,368 | 0 | A | T | 22.8% | 52.2 / 12.7 | 31 | intergenic (+35/+31) | pdxJ/PP_1437 | pyridoxine 5'‑phosphate synthase/heavy metal sensor histidine kinase |
| Reads supporting (aligned to +/- strand): ref base A (14/9); new base T (3/4); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.56e-01 | |||||||||||
AATGAGTTGAACATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAAC > NC_002947/1637229‑1637510 | aaTGAGTTGAACATTGGCCATGCGCTGGTGGCGCAAGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAag > 2:89197/1‑150 (MQ=255) tGAGTTGAACATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAagag > 2:540288/1‑150 (MQ=255) tGAGTTGAACATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGAGCTTGCCCTCGAaaag < 2:72890/150‑1 (MQ=255) cATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGcaca < 2:215358/150‑1 (MQ=255) tGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGCTTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCcgc > 2:26660/1‑132 (MQ=255) tGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCcgc < 1:26660/132‑1 (MQ=255) gcgcTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTCTTCGCGGGCTTGCCCGCGAAAAGGCCGGTACAGGCGAccaac < 1:58164/150‑4 (MQ=37) gtggCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCg < 1:390394/150‑1 (MQ=255) cATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGa > 1:402391/1‑150 (MQ=255) ggCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGGAAACCGGGGAgg > 1:16961/1‑150 (MQ=255) tgGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCa > 2:514229/1‑116 (MQ=255) tgGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCa < 1:514229/116‑1 (MQ=255) gCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCACGCACGCGAAGAGGCCAGCACAGGGTGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAggggg < 1:537184/150‑1 (MQ=255) ccGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGc > 1:100374/1‑150 (MQ=255) gcTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCTTGCCCGCGaaaa < 1:242166/71‑3 (MQ=37) gcTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCTTGCCCGCGaaaa > 2:242166/1‑69 (MQ=37) ttCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCgg < 1:515480/106‑1 (MQ=255) ttCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCgg > 2:515480/1‑106 (MQ=255) ttCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATg > 1:229184/1‑150 (MQ=255) cgcTGATTGTTGGTAGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAAc > 2:585453/1‑150 (MQ=255) ttgGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATc < 2:402391/150‑1 (MQ=255) ttgGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATc < 1:89197/150‑1 (MQ=255) tCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAggg < 1:564273/102‑1 (MQ=255) tCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAggg > 2:564273/1‑102 (MQ=255) ccTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCAGCGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCa > 2:13527/1‑150 (MQ=255) cGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCa < 1:580745/131‑1 (MQ=255) cGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCa > 2:580745/1‑131 (MQ=255) cctcttCGCGGGCTTGCCCGCGAAAAGGCCGGTACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCa < 1:316213/124‑1 (MQ=37) ccTCTTCGCGGGCTTGCCCGCGAAAAGGCCGGTACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCa > 2:316213/1‑128 (MQ=37) ctcttCGCGGGCTTGCCCGCGAAAAGGCCGGTACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaac > 1:59130/4‑150 (MQ=38) gcgGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGccaaccccaac > 1:390000/1‑150 (MQ=255) | AATGAGTTGAACATTGGCCATGCGCTGGTGGCGCATGCCTTGTTTGTCGGCTTCAAGGCTGCTGTGGCCGAGATGAAGGCGCTGATCGTGGCGGCTTCGCGCTGATTGTTGGTCGCCTGTACCGGCCTTTTCGCGGGCAAGCCCGCGAAGAGGCCAGCACAGGCGGCCGATCACTCGGCAGGAAACACCAGGGTAAACCGGGTAGGCCCCAGGGGGCAGCTGCTGACCTCGACCCGCCCGCCATGCAACTGCATGATCGACTTCACGATCGCCAACCCCAAC > NC_002947/1637229‑1637510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |